Changes in morphology of actin filaments and expression of alkaline phosphatase at 3D cultivation of MG-63 osteoblast-like cells on mineralized fibroin scaffolds

A. V. Goncharenko; N. V. Malyuchenko; A. M. Moisenovich; M. S. Kotlyarova; A. Yu. Arkhipova; A. S. Kon’kov; I. I. Agapov; A. V. Molochkov; M. M. Moisenovich; M. P. Kirpichnikov

doi:10.1134/S1607672916050197

Changes in morphology of actin filaments and expression of alkaline phosphatase at 3D cultivation of MG-63 osteoblast-like cells on mineralized fibroin scaffolds

Authors: Goncharenko A.V.¹, Malyuchenko N.V.¹, Moisenovich A.M.¹, Kotlyarova M.S.¹, Arkhipova A.Y.¹, Kon’kov A.S.¹, Agapov I.I.², Molochkov A.V.³, Moisenovich M.M.¹, Kirpichnikov M.P.¹
Affiliations:
1. Faculty of Biology
2. Shumakov Research Institute of Transplantation and Artificial Organs
3. Vladimirskii Moscow Regional Research Clinical Institute
Issue: Vol 470, No 1 (2016)
Pages: 368-370
Section: Biochemistry, Biophysics, and Molecular Biology
URL: https://journal-vniispk.ru/1607-6729/article/view/211638
DOI: https://doi.org/10.1134/S1607672916050197
ID: 211638

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Abstract

3D cultivation of MG-63 osteoblast-like cells on mineralized fibroin scaffolds leads to an increase in the expression of alkaline phosphatase, an early marker of bone formation. Increased expression is associated with the actin cytoskeleton reorganization under the influence of 3D cultivation and osteogenic calcium phosphate component of the microcarrier.