Email this article Involvement of two-pore channels in hydrogen peroxide-induced increase in the level of calcium ions in the cytoplasm of human umbilical vein endothelial cells
- Authors: Avdonin P.V.1, Nadeev A.D.2,3, Tsitrin E.B.1, Tsitrina A.A.1, Avdonin P.P.1, Mironova G.Y.1, Zharkikh I.L.4, Goncharov N.V.3,5
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Affiliations:
- Koltzov Institute of Developmental Biology
- Institute of Biophysics, Pushchino Branch
- Sechenov Institute of Evolutionary Physiology and Biochemistry
- Research Institute of General Pathology and Pathophysiology
- Research Institute of Hygiene, Occupational Pathology, and Human Ecology
- Issue: Vol 474, No 1 (2017)
- Pages: 209-212
- Section: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journal-vniispk.ru/1607-6729/article/view/211902
- DOI: https://doi.org/10.1134/S1607672917030152
- ID: 211902
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Abstract
Hydrogen peroxide at concentrations below cytotoxic ones causes an increase in the cytoplasmic calcium concentration in human umbilical vein endothelial cells as a result of calcium release from intracellular stores. Two-pore calcium channel blocker trans-NED19 partially suppresses the increase in the level of calcium ions in the cells in response to the addition of hydrogen peroxide. The staining of endothelial cells with the fluorescent stereoisomer cis-NED19 and LysoTracker confirmed the localization of two-pore calcium channels in lysosomes and endolysosomal vesicles.
About the authors
P. V. Avdonin
Koltzov Institute of Developmental Biology
Author for correspondence.
Email: pvavdonin@yandex.ru
Russian Federation, Moscow, 119334
A. D. Nadeev
Institute of Biophysics, Pushchino Branch; Sechenov Institute of Evolutionary Physiology and Biochemistry
Email: pvavdonin@yandex.ru
Russian Federation, Pushchino, Moscow oblast, 142290; St. Petersburg, 194223
E. B. Tsitrin
Koltzov Institute of Developmental Biology
Email: pvavdonin@yandex.ru
Russian Federation, Moscow, 119334
A. A. Tsitrina
Koltzov Institute of Developmental Biology
Email: pvavdonin@yandex.ru
Russian Federation, Moscow, 119334
P. P. Avdonin
Koltzov Institute of Developmental Biology
Email: pvavdonin@yandex.ru
Russian Federation, Moscow, 119334
G. Yu. Mironova
Koltzov Institute of Developmental Biology
Email: pvavdonin@yandex.ru
Russian Federation, Moscow, 119334
I. L. Zharkikh
Research Institute of General Pathology and Pathophysiology
Email: pvavdonin@yandex.ru
Russian Federation, Moscow, 125315
N. V. Goncharov
Sechenov Institute of Evolutionary Physiology and Biochemistry; Research Institute of Hygiene, Occupational Pathology, and Human Ecology
Email: pvavdonin@yandex.ru
Russian Federation, St. Petersburg, 194223; Kapitolovo, Leningrad oblast, 188663
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