Homeotic MADS-box genes encoding LeMADS-MC orthologues in wild tomato species (genus Solanum)


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Abstract

New full-length genes encoding the LeMADS-MC transcription factor orthologues were identified and functionally characterized in wild tomato species S. cheesmaniae and S. habrochaites. A comparative analysis of the encoded proteins and LeMADS-MC of the cultivated tomato species S. lycopersicum revealed two major amino acid residues substitutions: V155E in the K-domain of ShaMADS-MC (S. habrochaites) and S80L in the I-region of SchMADS-MC (S. cheesmaniae). Structural differences of the C-terminal regions of MC and the canonical euAP1 proteins indicate possible chromosomal rearrangements in the Solanoideae subfamily, which, however, did not change the main known conserved euAP1 functions.

About the authors

M. A. Slugina

Institute of Bioengineering, Research Center of Biotechnology; Faculty of Biotechnology

Author for correspondence.
Email: machinmail@mail.ru
Russian Federation, Moscow, 119071; Moscow, 119992

E. Z. Kochieva

Institute of Bioengineering, Research Center of Biotechnology; Faculty of Biotechnology

Email: machinmail@mail.ru
Russian Federation, Moscow, 119071; Moscow, 119992

K. G. Skryabin

Institute of Bioengineering, Research Center of Biotechnology; Faculty of Biotechnology

Email: machinmail@mail.ru
Russian Federation, Moscow, 119071; Moscow, 119992

A. V. Shchennikova

Institute of Bioengineering, Research Center of Biotechnology

Email: machinmail@mail.ru
Russian Federation, Moscow, 119071

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