Detection of human neuronal α7 nicotinic acetylcholine receptors by conjugates of snake α-neurotoxin with quantum dots

Ya. V. Makarova; I. V. Shelukhina; A. K. Mukherjee; D. V. Kuznetsov; V. I. Tsetlin; Yu. N. Utkin

doi:10.1134/S1607672917040032

Detection of human neuronal α7 nicotinic acetylcholine receptors by conjugates of snake α-neurotoxin with quantum dots

Authors: Makarova Y.V.¹, Shelukhina I.V.¹, Mukherjee A.K.², Kuznetsov D.V.³, Tsetlin V.I.¹, Utkin Y.N.¹^,3
Affiliations:
1. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
2. Tezpur University
3. The National University of Science and Technology MISiS
Issue: Vol 475, No 1 (2017)
Pages: 253-255
Section: Biochemistry, Biophysics, and Molecular Biology
URL: https://journal-vniispk.ru/1607-6729/article/view/211939
DOI: https://doi.org/10.1134/S1607672917040032
ID: 211939

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Abstract

Fluorescent derivatives are widely used to study the structure and functions of proteins. Quantum dots (QDs), fluorescent semiconductor nanocrystals, have a high quantum yield and are much more resistant to bleaching compared to organic dyes. Conjugates of α-neurotoxins with QDs were used for visualization of human α7 acetylcholine receptors heterologously expressed in GH4C1 pituitary adenoma cells. Specific staining of cells by the conjugated toxins was observed.