Synthesis and Membrane Transport of Serotonin in the Developing Ovarian Follicle of Mouse


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Abstract

Using RT-PCR, we showed the presence of Tph1 mRNA in follicular cells and Tph2 mRNA in oocytes isolated from primary multilayer ovarian follicles of mouse and the absence of Ddc expression, which indicates that serotonin cannot be synthesized in a developing ovarian follicle. The membrane serotonin transporter Sert is expressed in follicular cells and oocytes. Experiments on the cultivation of follicles in vitro confirmed the absence of serotonin synthesis from 5-hydroxytryptophan and the presence of membrane transport activity in the oocyte.

About the authors

D. A. Nikishin

Koltzov Institute of Developmental Biology; Moscow State University

Author for correspondence.
Email: denisnikishin@gmail.com
Russian Federation, ul. Vavilova 26, Moscow, 119334; Moscow, 119992

N. M. Alyoshina

Moscow State University

Email: denisnikishin@gmail.com
Russian Federation, Moscow, 119992

Y. B. Shmukler

Koltzov Institute of Developmental Biology

Email: denisnikishin@gmail.com
Russian Federation, ul. Vavilova 26, Moscow, 119334

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