Polyarginine Peptides As a New Class of Ligands of Nicotinic Acetylcholine Receptors


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Abstract

Arginine-containing peptides R3, R8, and R16 were obtained by solid-phase peptide synthesis, and their binding to nicotinic acetylcholine receptors (nAChRs) of muscle and neuronal (α7) types was studied by competitive radioligand assay with the use of 125I-α-bungarotoxin. The resulting peptides exhibited a significantly greater binding activity with respect to the muscle-type nAChRs than to the α7 receptor. Thus, we have discovered a new class of nAChR ligands. The affinity of the synthesized oligoarginines for nAChR depended on the number of amino acid residues in the chain. The highest affinity was exhibited by the R16 peptide, which contained 16 arginine residues.

About the authors

E. V. Kryukova

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry

Author for correspondence.
Email: evkr@mail.ru
Russian Federation, Moscow, 117997

I. A. Ivanov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry

Email: evkr@mail.ru
Russian Federation, Moscow, 117997

D. S. Lebedev

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry

Email: evkr@mail.ru
Russian Federation, Moscow, 117997

E. N. Spirova

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry

Email: evkr@mail.ru
Russian Federation, Moscow, 117997

D. A. Senko

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry

Email: evkr@mail.ru
Russian Federation, Moscow, 117997

N. S. Egorova

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry

Email: evkr@mail.ru
Russian Federation, Moscow, 117997

I. E. Kasheverov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry; Sechenov First Moscow State Medical University

Email: evkr@mail.ru
Russian Federation, Moscow, 117997; Moscow, 119992

V. I. Tsetlin

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry

Email: evkr@mail.ru
Russian Federation, Moscow, 117997

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