Conversion of Tomato Allene Oxide Synthase LeAOS3 (CYP74C3) into Epoxyalcohol Synthase by Site-Directed Mutagenesis

S. S. Gorina; E. O. Smirnova; L. S. Mukhtarova; Y. Y. Toporkova; A. N. Grechkin

doi:10.1134/S1607672918060108

Conversion of Tomato Allene Oxide Synthase LeAOS3 (CYP74C3) into Epoxyalcohol Synthase by Site-Directed Mutagenesis

Authors: Gorina S.S.¹, Smirnova E.O.¹, Mukhtarova L.S.¹, Toporkova Y.Y.¹, Grechkin A.N.¹
Affiliations:
1. Kazan Institute of Biochemistry and Biophysics
Issue: Vol 483, No 1 (2018)
Pages: 329-332
Section: Biochemistry, Biophysics, and Molecular Biology
URL: https://journal-vniispk.ru/1607-6729/article/view/212552
DOI: https://doi.org/10.1134/S1607672918060108
ID: 212552

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Abstract

The site-directed mutagenesis of tomato allene oxide synthase LeAOS3 (CYP74C3) on the “F/L toggle” site resulted in a partial conversion of LeAOS3 into epoxyalcohol synthase.