Ectopic Expression of the Homeotic MADS-Box Gene HAM31 (Helianthus annuus L.) in Transgenic Plants Nicotiana tabacum L. Affects the Gynoecium Identity
- Authors: Shchennikova A.V.1, Shulga O.A.1,2, Skryabin K.G.1
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Affiliations:
- Institute of Bioengineering, Research Center of Biotechnology
- All-Russia Research Institute of Agricultural Biotechnology
- Issue: Vol 483, No 1 (2018)
- Pages: 363-368
- Section: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journal-vniispk.ru/1607-6729/article/view/212624
- DOI: https://doi.org/10.1134/S1607672918060182
- ID: 212624
Cite item
Abstract
The structure of the MADS-box gene HAM31 of the sunflower (Helianthus annuus) was characterized. It is shown that the product of this gene is an ortholog of the B-class MADS transcription factor PISTILLATA (Arabidopsis thaliana). Two types of transgenic tobacco plants (Nicotiana tabacum) with the constitutive expression of the HAM31 gene in the sense and antisense orientation were obtained. The 35S::HAM31s plants formed flowers with an altered gynoecium identity, whereas 35S::HAM31as plants did not differ from the control.
About the authors
A. V. Shchennikova
Institute of Bioengineering, Research Center of Biotechnology
Author for correspondence.
Email: shchennikova@yandex.ru
Russian Federation, Moscow, 119071
O. A. Shulga
Institute of Bioengineering, Research Center of Biotechnology; All-Russia Research Institute of Agricultural Biotechnology
Email: shchennikova@yandex.ru
Russian Federation, Moscow, 119071; Moscow
K. G. Skryabin
Institute of Bioengineering, Research Center of Biotechnology
Email: shchennikova@yandex.ru
Russian Federation, Moscow, 119071
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