Ectopic Expression of the Homeotic MADS-Box Gene HAM31 (Helianthus annuus L.) in Transgenic Plants Nicotiana tabacum L. Affects the Gynoecium Identity


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Abstract

The structure of the MADS-box gene HAM31 of the sunflower (Helianthus annuus) was characterized. It is shown that the product of this gene is an ortholog of the B-class MADS transcription factor PISTILLATA (Arabidopsis thaliana). Two types of transgenic tobacco plants (Nicotiana tabacum) with the constitutive expression of the HAM31 gene in the sense and antisense orientation were obtained. The 35S::HAM31s plants formed flowers with an altered gynoecium identity, whereas 35S::HAM31as plants did not differ from the control.

About the authors

A. V. Shchennikova

Institute of Bioengineering, Research Center of Biotechnology

Author for correspondence.
Email: shchennikova@yandex.ru
Russian Federation, Moscow, 119071

O. A. Shulga

Institute of Bioengineering, Research Center of Biotechnology; All-Russia Research Institute of Agricultural Biotechnology

Email: shchennikova@yandex.ru
Russian Federation, Moscow, 119071; Moscow

K. G. Skryabin

Institute of Bioengineering, Research Center of Biotechnology

Email: shchennikova@yandex.ru
Russian Federation, Moscow, 119071

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