Email this article Optimization of Heterologous Expression of Insulin Receptor-Related Receptor Ectodomain
- Authors: Mozhaev A.A.1,2, Orsa A.N.1, Deyev I.E.1, Shvets V.I.3, Petrenko A.G.1
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Affiliations:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Shubnikov Institute of Crystallography, Crystallography and Photonics Federal Research Center, Russian Academy of Sciences
- Institute of Fine Chemical Technology, Russian Technological University (MIREA)
- Issue: Vol 485, No 1 (2019)
- Pages: 101-103
- Section: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journal-vniispk.ru/1607-6729/article/view/212880
- DOI: https://doi.org/10.1134/S1607672919020017
- ID: 212880
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Abstract
In this paper, we present an approach to optimize the heterologous expression of the receptor tyrosine kinase IRR, which further simplifies the purification of the IRR from the medium and increases the final yield. The approach proposed by us can find application in the biotechnological production of other large-scale recombinant proteins produced for medical purposes.
About the authors
A. A. Mozhaev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Shubnikov Institute of Crystallography, Crystallographyand Photonics Federal Research Center, Russian Academy
of Sciences
Author for correspondence.
Email: a.a.mozhaev@gmail.com
Russian Federation, Moscow, 117997; Moscow, 117333
A. N. Orsa
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: a.a.mozhaev@gmail.com
Russian Federation, Moscow, 117997
I. E. Deyev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: a.a.mozhaev@gmail.com
Russian Federation, Moscow, 117997
V. I. Shvets
Institute of Fine Chemical Technology, Russian Technological University (MIREA)
Email: a.a.mozhaev@gmail.com
Russian Federation, Moscow, 117571
A. G. Petrenko
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: a.a.mozhaev@gmail.com
Russian Federation, Moscow, 117997
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