Email this article Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings
- Authors: Bolsunovsky A.Y.1, Dementyev D.V.1, Frolova T.S.2,3, Trofimova E.A.1, Iniatkina E.M.1, Vasilyev S.A.4, Sinitsyna O.I.2,3
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Affiliations:
- Institute of Biophysics, Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences
- Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences
- Novosibirsk State University
- Research Institute of Medical Genetics, Tomsk National Research Medical Center, Russian Academy of Sciences
- Issue: Vol 489, No 1 (2019)
- Pages: 362-366
- Section: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journal-vniispk.ru/1607-6729/article/view/213108
- DOI: https://doi.org/10.1134/S1607672919060024
- ID: 213108
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Abstract
The effect of γ-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (≤ 0.1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0.1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose γ-radiation on Allium cepa seedlings.
About the authors
A. Ya. Bolsunovsky
Institute of Biophysics, Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences
Author for correspondence.
Email: radecol@ibp.ru
Russian Federation, Krasnoyarsk, 660036
D. V. Dementyev
Institute of Biophysics, Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences
Email: radecol@ibp.ru
Russian Federation, Krasnoyarsk, 660036
T. S. Frolova
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences; Novosibirsk State University
Email: radecol@ibp.ru
Russian Federation, Novosibirsk, 630090; Novosibirsk, 630090
E. A. Trofimova
Institute of Biophysics, Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences
Email: radecol@ibp.ru
Russian Federation, Krasnoyarsk, 660036
E. M. Iniatkina
Institute of Biophysics, Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences
Email: radecol@ibp.ru
Russian Federation, Krasnoyarsk, 660036
S. A. Vasilyev
Research Institute of Medical Genetics, Tomsk National Research Medical Center, Russian Academy of Sciences
Email: radecol@ibp.ru
Russian Federation, Tomsk, 634058
O. I. Sinitsyna
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences; Novosibirsk State University
Email: radecol@ibp.ru
Russian Federation, Novosibirsk, 630090; Novosibirsk, 630090
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