Antigenotoxic activity of the combination «aspartame + betaine» and metformin in mice with experimental streptozotocin diabetes
- Autores: Lisitsyn A.A.1,2, Zhanataev A.K.1, Chaika Z.V.1, Sevostyanova E.M.3, Artamonova M.P.2, Chernukha I.M.4, Oganesyants L.A.3, Durnev A.D.1
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Afiliações:
- V.V. Zakusov Research Institute of Pharmacology
- Moscow State University of Food Production
- All-Russian Research Institute of Brewing, Non-Alcoholic and Wine-making Industry - a branch of the Federal State Budget Scientific Institution «V.M. Gorbatov Federal Research Center for Food Systems of RAS»
- V.M. Gorbatov Federal Research Center for Food Systems of RAS
- Edição: Volume 20, Nº 5 (2022)
- Páginas: 59-64
- Seção: Articles
- URL: https://journal-vniispk.ru/1728-2918/article/view/143963
- DOI: https://doi.org/10.29296/24999490-2022-05-08
- ID: 143963
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Resumo
Introduction. An increase in DNA damage (genotoxicity) is characteristic of patients with diabetes mellitus. The main complications in diabetes are accompanied and/or initiated by DNA damage. Hence, the necessity to search for and study compounds with antigenotoxic properties for use in diabetes mellitus is obvious. Aim. Study of the antigenotoxic activity of aspartame and betaine combination in a mice diabetes mellitus model in comparison with the well-known hypoglycemic drug metformin. Material and methods. DNA damage was estimated using the «DNA comet» assay. Streptozotocin was used as a diabetogenic agent. Aspartame (35 mg/l) and betaine (175 mg/l) were given in the drinking water throughout the experiment. Metformin at a dose of 250 mg/kg was administered orally at the same time. The study was performed on BALB/c male mice. Results. After a single i.p. administration of 200 mg/kg streptozotocin or after its five-day daily administration at a dose of 40 mg/kg, hyperglycemia was observed, as well as DNA damage in the liver and kidney cells, but not in the brain, pancreas and testes cells. During the single streptozotocin injection experiment DNA damage under the influence of aspartame and betaine decreased by 58.0% in liver cells and by 53.8% in kidney cells. Metformin reduced DNA damage by 44.2 and 71.0%, respectively, in liver and kidney cells. During the repeated streptozotocin administration experiment aspartame and betaine reduced DNA damage by 52.2% in liver cells and by 46.6% in kidney cells. Metformin reduced DNA damage by 30.8 and 38.3%, respectively, in liver and kidney cells. Discussion. The data obtained demonstrate the antigenotoxic effect of the aspartame and betaine combination in experimental diabetes mellitus and confirm a similar activity in metformin, previously identified in a single study.
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##article.viewOnOriginalSite##Sobre autores
Artem Lisitsyn
V.V. Zakusov Research Institute of Pharmacology; Moscow State University of Food Production
Autor responsável pela correspondência
Email: nordikal@yandex.ru
Junior Researcher; Postgraduate student; Department of «Designing Functional Food Products and Nutritionology»
Baltijskaya ul. 8, Moscow, 125315, Russian Federation; Volokolamskoe shosse, 11, Moscow, 125080, Russian FederationAliy Zhanataev
V.V. Zakusov Research Institute of Pharmacology
Email: zhanataev@academpharm.ru
leading researcher; Candidate of Biological Science Baltijskaya ul. 8, Moscow, 125315, Russian Federation
Zlata Chaika
V.V. Zakusov Research Institute of Pharmacology
Email: pcr201069@yandex.ru
researcher Baltijskaya ul. 8, Moscow, 125315, Russian Federation
Elena Sevostyanova
All-Russian Research Institute of Brewing, Non-Alcoholic and Wine-making Industry - a branch of the Federal State Budget Scientific Institution «V.M. Gorbatov Federal Research Center for Food Systems of RAS»
Email: waterlena@list.ru
leading researcher; Candidate of Biological Science. ul. Rossolimo, 7, Moscow, 119021, Russian Federation
Marina Artamonova
Moscow State University of Food Production
Email: artamonovamp@mgupp.ru
Associate Professor; Department of «Designing Functional Foods and Nutritionology», candidate of Technical Sciences Volokolamskoe shosse, 11, Moscow, 125080, Russian Federation
Irina Chernukha
V.M. Gorbatov Federal Research Center for Food Systems of RAS
Email: imcher@inbox.ru
Principal researcher, Experimental clinic-laboratory of biologically active substances of animal origin; Doctor of Technical Sciences, Professor, Academician of RAS ul. Talalihina, 26, Moscow, 109316, Russian Federation
Lev Oganesyants
All-Russian Research Institute of Brewing, Non-Alcoholic and Wine-making Industry - a branch of the Federal State Budget Scientific Institution «V.M. Gorbatov Federal Research Center for Food Systems of RAS»
Email: vniipbivp@fncps.ru
Scientific Director of Institute; Doctor of Technical Sciences, Professor, Academician of RAS ul. Rossolimo, 7, Moscow, 119021, Russian Federation
Andrey Durnev
V.V. Zakusov Research Institute of Pharmacology
Email: addurnev@mail.ru
expert scientific worker; Doctor of Medical Sciences, Professor, Corresponding Member of the RAS. Baltijskaya ul. 8, Moscow, 125315, Russian Federation
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