Email this article The effects of prolonged formalin fixation on immunohistochemical staining of PCNA and Dcx proteins in the mouse hippocampus
- Authors: Aniol V.A.1
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Affiliations:
- Institute of Higher Nervous Activity and Neurophysiology
- Issue: Vol 10, No 3 (2016)
- Pages: 244-247
- Section: Methods
- URL: https://journal-vniispk.ru/1819-7124/article/view/211115
- DOI: https://doi.org/10.1134/S181971241603003X
- ID: 211115
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Abstract
We have performed a quantitative analysis of temporal pattern of immunohistochemical staining to PCNA and Dcx in brain tissue under the influence of 10% neutral buffered formalin (fixation for 3.5 h–7 weeks). After 7 days of fixation we found a decrease in Dcx and, to a lesser extent, PCNA immunoreactivity. Later, no further decline in immunoreactivity occurred. Our data may be useful for optimization of neurogenesis assessment protocols in mammalian and human brains.
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About the authors
V. A. Aniol
Institute of Higher Nervous Activity and Neurophysiology
Author for correspondence.
Email: aniviktor@narod.ru
Russian Federation, Moscow
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