Neuronal Networks on Multielectrode Arrays as High Sensitive Biosensors of the Functional State of Nerve Cells
- Authors: Kapkaeva M.R.1, Genrikhs E.E.1, Stelmashook E.V.1, Kondratenko R.V.1, Isaev N.K.1,2,3, Khaspekov L.G.1
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Affiliations:
- Research Center of Neurology
- Department of Biology
- Belozersky Institute of Physico-chemical Biology
- Issue: Vol 12, No 3 (2018)
- Pages: 234-238
- Section: Articles
- URL: https://journal-vniispk.ru/1990-7478/article/view/213320
- DOI: https://doi.org/10.1134/S1990747818030054
- ID: 213320
Cite item
Abstract
The dynamics of changes of spontaneous neuronal activity in primary hippocampal cell cultures developing on a multielectrode array (MEA) was studied using a multielectrode system. The intensification of bioelectrical activity, which depended on the duration of cultivation and stabilized by the second week in vitro, has been revealed. An increase in the concentration of glutamate (up to 2 μM) by addition into the incubation medium during the stabilization period resulted in a rapid and significant reorganization of the neuronal network activity pattern. On the other hand, inhibition and subsequent gradual recovery of the neuronal network activity immediately after the neurotransmitter addition at higher concentrations (50 or 100 μM) have been observed. At the same time, on some electrodes in the presence of high doses of glutamate (100 μM), a complete or partially irreversible suppression of the activity has been recorded. A significant reduction in spontaneous activity of the neuronal network, which was not accompanied by neuronal death, also occurred when copper ions (Cu2+) at a concentration of 10 μM were added into the incubation medium for 48 h. The obtained data demonstrate high biosensor sensitivity of the neuronal network cultured on the multielectrode array, which makes it possible to use it as an effective test system for studies of biologically active compounds.
About the authors
M. R. Kapkaeva
Research Center of Neurology
Email: estelmash@mail.ru
Russian Federation, Moscow, 125367
E. E. Genrikhs
Research Center of Neurology
Email: estelmash@mail.ru
Russian Federation, Moscow, 125367
E. V. Stelmashook
Research Center of Neurology
Author for correspondence.
Email: estelmash@mail.ru
Russian Federation, Moscow, 125367
R. V. Kondratenko
Research Center of Neurology
Email: estelmash@mail.ru
Russian Federation, Moscow, 125367
N. K. Isaev
Research Center of Neurology; Department of Biology; Belozersky Institute of Physico-chemical Biology
Email: estelmash@mail.ru
Russian Federation, Moscow, 125367; Moscow, 119991; Moscow, 119991
L. G. Khaspekov
Research Center of Neurology
Email: estelmash@mail.ru
Russian Federation, Moscow, 125367
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