Enzymatic interesterification of sunflower oil and hydrogenated soybean oil with the immobilized bacterial recombinant lipase from Geobacillus stearothermophilus G3

Abstract

In this study we discuss the properties of biocatalyst based on Geobacillus stearothermophilus G3 lipase immobilized on silica gel. Interesterification of sunflower oil and hydrogenated soybean oil (HSO) in a batch reaction was completed within 2 h, 10% biocatalyst (wt %), 70°С, and HSO: sunflower oil molar ratio of 1: 3. The physical and chemical parameters of reaction products were studied by HPLC-MS to access the composition of triacylglycerides and by slip melting point method. Biocatalyst has shown high operational stability. The estimated time of its operation in the oil mixture was 243 h that makes this catalyst promising for application in manufacturing of modified fats, including fats with high melting temperatures.