Enhancing the Yield of Bioethanol from the Lignocellulose of Oat Hulls by Optimizing the Composition of the Nutrient Medium
- Authors: Skiba E.A.1, Mironova G.F.1, Kukhlenko A.A.1, Orlov S.E.1
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Affiliations:
- Institute for Problems of Chemical and Energetic Technologies, Siberian Branch, Russian Academy of Sciences
- Issue: Vol 10, No 3 (2018)
- Pages: 257-262
- Section: Biocatalysis
- URL: https://journal-vniispk.ru/2070-0504/article/view/202911
- DOI: https://doi.org/10.1134/S207005041803008X
- ID: 202911
Cite item
Abstract
The optimum composition is determined for a nutrient medium corresponding to the maximum yield of bioethanol during the alcohol fermentation of the enzymatic hydrolyzate of lignocellulosic material, produced by treating oat hulls with a diluted solution of nitric acid in trial production. The biotechnological steps of saccharification and fermentation are performed using commercially available enzymatic preparations CelloLux-A and BrewZyme BGX, plus a strain of Saccharomyces сerevisiae Y-1693 (VKPM) that is resistant to inhibitors from hydrolyzates. The composition of a nutrient medium that produced a bioethanol yield which was 89.9% of the theoretical one (8.4% higher than the native hydrolyzate) is found to have a 1.82 g/L concentration of ammonium sulfate, a 0.98 g/L concentration of potassium monophosphate, and a 6.47 g/L concentration of yeast extract.
About the authors
E. A. Skiba
Institute for Problems of Chemical and Energetic Technologies, Siberian Branch,Russian Academy of Sciences
Author for correspondence.
Email: eas08988@mail.ru
Russian Federation, Biysk, 659332
G. F. Mironova
Institute for Problems of Chemical and Energetic Technologies, Siberian Branch,Russian Academy of Sciences
Email: eas08988@mail.ru
Russian Federation, Biysk, 659332
A. A. Kukhlenko
Institute for Problems of Chemical and Energetic Technologies, Siberian Branch,Russian Academy of Sciences
Email: eas08988@mail.ru
Russian Federation, Biysk, 659332
S. E. Orlov
Institute for Problems of Chemical and Energetic Technologies, Siberian Branch,Russian Academy of Sciences
Email: eas08988@mail.ru
Russian Federation, Biysk, 659332
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