Study of morphological transformation and features of vascular blood flow of the wall of the small and large intestine in the simulation of ischemia in the experiment
- 作者: Karpova I.Y.1, Peretyagin P.V.2, Orlinskaya N.Y.2, Shirokova N.Y.2, Pyatova E.D.2, Ptushko S.S.2
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隶属关系:
- Volga Research Medical University
- Privolzhsky Research Medical University
- 期: 卷 16, 编号 2 (2023)
- 页面: 120-129
- 栏目: Original articles
- URL: https://journal-vniispk.ru/2070-478X/article/view/233099
- DOI: https://doi.org/10.18499/2070-478X-2023-16-2-120-129
- ID: 233099
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Introduction. Ischemia of the small and large intestine of various degree was simulated in 45 sexually mature male rats of the Wistar line weighing 150-200 g on the basis of the Department of Experimental Medicine with vivarium at Privolzhsky Research Medical University.
Аim. To present in an experiment the effect of different degrees of occlusive ischemia on the morphological transformation of the intestinal wall and the level of changes in blood flow.
Materials and methods. The anesthetized animals underwent a median laparotomy with subsequent differentiation of the intestinal divisions: the jejunal section was selected in the small intestine, the ascending section was selected in the large intestine. With the help of a nylon thread (5-0), the blood supplying arcades of these zones were ligated and further exposed for 40, 60 and 120 minutes. During the indicated periods of ischemia, the average rate of blood supply in capillary microvessels located at a depth of 0.5-1.0 mm was estimated in relative units on 1.0 mm2-area (LACC-02, NPP Lazma, Russia). After the assessment of vascular blood flow was completed, ischemic intestinal areas were sampled for morphological examination. The study results were processed using Excel application and STADIA statistical package.
Results. In the course of study, the authors registered clear relationships between blood flow parameters in different parts of the intestine and the duration of ischemia. Local trophic disturbance was combined with a transformation in the histoarchitectonics of the intestinal wall. It is noted that adaptive-regenerative mechanisms provide tissue stress reduction in 120 min. due to compensatory mechanisms of blood supply contributing to the restoration of the "villus-crypt" system of the mucous membrane.
Conclusion. Thus, in case of local ischemia in the small and large intestine, the tissue structure is restored due to adaptive mechanisms of blood supply, this preserves the viability and functionality of the intestinal wall.
作者简介
Irina Karpova
Volga Research Medical University
Email: ikarpova73@mail.ru
ORCID iD: 0000-0002-4897-6702
SPIN 代码: 8464-8485
Doctor of Medical Sciences, Associate Professor, Department of Pediatric Surgery
俄罗斯联邦, 603005, Nizhny Novgorod, Minin and Pozharsky pl., 10/1Pyotr Peretyagin
Privolzhsky Research Medical University
Email: peretyaginpv@gmail.com
ORCID iD: 0000-0003-0707-892X
SPIN 代码: 9241-1854
Junior Researcher of the Department of Physico-Chemical Research of the Central Research Institute
俄罗斯联邦, Nizhny Novgorod, Minin Square, 10/5Natalia Orlinskaya
Privolzhsky Research Medical University
Email: orlinskaya@rambler.ru
ORCID iD: 0000-0003-2896-2968
SPIN 代码: 3540-4182
Scopus 作者 ID: 33156677
Professor, Head of the Department of Pathological Anatomy
俄罗斯联邦, 603005, Nizhny Novgorod, Minina pl. 10/1Natalia Shirokova
Privolzhsky Research Medical University
Email: nush63@mail.ru
ORCID iD: 0000-0002-6242-5958
Senior Researcher, Pathological Anatomy Group
俄罗斯联邦, Minin and Pozharsky pl., 10/1, N. Novgorod, 603005, Russian FederationEvgeniya Pyatova
Privolzhsky Research Medical University
Email: edpyatova@mail.ru
ORCID iD: 0000-0001-7501-363X
SPIN 代码: 2763-9209
Senior Lecturer, Department of Medical Physics and Informatics
俄罗斯联邦, Minin and Pozharsky pl., 10/1, N. Novgorod, 603005, Russian FederationSofia Ptushko
Privolzhsky Research Medical University
编辑信件的主要联系方式.
Email: ptushkosofia@email.com
ORCID iD: 0000-0002-1278-9497
5th Year Medical Student
俄罗斯联邦, Minin and Pozharsky pl., 10/1, N. Novgorod, 603005, Russian Federation参考
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