Email this article Study of Hematopoietic Stem Cells in Patients with Extensive Burns
- Authors: Kozlova M.N.1, Zemskov V.M.2, Alekseev A.A.2,3, Demidova V.S.2, Shishkina N.S.2, Kulikova A.N.2, Zemskov A.M.4, Bobrovnikov A.E.2,3
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Affiliations:
- A.V. Vishnevsky National Medical Research Center of Surgery
- A.V. Vishnevsky National Medical Research Center of Surgery, Mosсow, Russia
- Federal State Budgetary Educational Institution of Further Professional Education "Russian Medical Academy of Continuous Professional Education" of the Ministry of Healthcare of the Russian Federation, Mosсow, Russia
- N.N. Burdenko Voronezh State Medical University,Voronezh, Russia
- Issue: Vol 15, No 4 (2022)
- Pages: 300-305
- Section: Original articles
- URL: https://journal-vniispk.ru/2070-478X/article/view/233325
- DOI: https://doi.org/10.18499/2070-478X-2022-15-4-300-305
- ID: 233325
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Abstract
Introduction. Assessment of tissue regeneration, including that at the cellular and subcellular levels, appears to be one of the important trends in the complex treatment of patients with extensive burns. It is known that hematopoietic stem cells (HSCs) contribute to tissue restoration and regeneration through paracrine effects or direct cell differentiation, being a central component of post-burn anemia recovery and capable of forming not only blood cells, but also other types of cells. Notably, the role of these cells in burn injury has not been studied yet.
The aim of the study was to investigate in dynamics the content of hematopoietic stem cells of different phenotypes in patients with extensive burns in the process of complex treatment.
Methods. Hematopoietic stem cells and their subpopulations in peripheral blood samples were analyzed on a FACSCalibur flow cytometer (Becton Dickinson, USA) using the CellQuest program and CD45/CD34/CD38 and CD45/CD34/CD133 monoclonal antibody panels (BD, USA). The results obtained were statistically processed using the GraphPad Prism 7.0 program (USA), results were considered statistically significant at p<0.05.
Results. Hematopoietic stem cells and their subpopulations were studied at different stages of the complex treatment in 25 patients with a large burn area, more than 30% of the body surface. The comparison group consisted of 15 healthy volunteers. Upon admission to the Burn Center, a group of severely burned patients revealed a significant deep deficiency of both total HSCs CD45+34+ (p=0.0002) and their subpopulations CD45dim34+38+ (p=0.019), with predominantly early precursors of hematopoiesis CD45dim34+38- (p=0.0001) and CD45dim34+133+ (p=0.0002). In the course of the complex treatment, including surgical necrectomy and autodermoplasty of burn wounds, there was observed normalization of total HSCs CD45+34+ (0.05±0.012%, p=0.031) and a subpopulation of early HSCs CD45dim34+38- (0.039±0.009%, p=0.016) in 20 days of treatment in the group of burn patients. There was a significant increase of mature CD45dim34+133- HSCs (p=0.0380) as a result of treatment, while the deficit of the more differentiated population of CD45dim34+38+ HSCs did not fully recover (p=0.272).
Conclusion. The firstly detected modulations in the content of hematopoietic stem cells of different phenotypes in patients with extensive burns may reflect the state of compensatory-adaptive reactions of hematopoiesis in the course of the complex treatment. The data obtained may support the predictive use of HSC subcellular markers to assess the regenerative potential in burn wounds healing, including that during staged surgical treatment and autodermoplasty, and to predict the development of local and general complications of burn disease.
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##article.viewOnOriginalSite##About the authors
Maria Nikolaevna Kozlova
A.V. Vishnevsky National Medical Research Center of Surgery
Email: mnkozlova@rambler.ru
ORCID iD: 0000-0002-0554-7094
SPIN-code: 7631-8050
Scopus Author ID: 57194480001
PhD, Senior Researcher of the Clinical Diagnostic Laboratory
Russian Federation, 117997, Russia, Moscow, B. Serpukhovskaya street, 27
Vladimir Mikhailovich Zemskov
A.V. Vishnevsky National Medical Research Center of Surgery, Mosсow, Russia
Email: arturrego@yandex.ru
ORCID iD: 0000-0002-8867-5349
SPIN-code: 7754-0336
Scopus Author ID: 7103049738
MD, Professor, Chief Researcher of the Clinical Diagnostic Laboratory
Russian Federation, 117997, Russia, Moscow, B. Serpukhovskaya street, 27Andrey Anatolyevich Alekseev
A.V. Vishnevsky National Medical Research Center of Surgery, Mosсow, Russia;Federal State Budgetary Educational Institution of Further Professional Education "Russian Medical Academy of Continuous Professional Education" of the Ministry of Healthcare of the Russian Federation, Mosсow, Russia
Email: alexseev@ixv.ru
ORCID iD: 0000-0001-6675-4794
SPIN-code: 4803-3939
Scopus Author ID: 720289106
MD, Professor, Deputy Director
Russian Federation, 117997, Russia, Moscow, B. Serpukhovskaya street, 27Valentina Semenova Demidova
A.V. Vishnevsky National Medical Research Center of Surgery, Mosсow, Russia
Email: demidova@ixv.ru
ORCID iD: 0000-0003-3187-4408
SPIN-code: 5611-4653
Scopus Author ID: 56977389500
MD, Head of Clinical Diagnostic Laboratory Russian Federation, 117997, Russia, Moscow, B. Serpukhovskaya street, 27
Nadezhda Semenovna Shishkina
A.V. Vishnevsky National Medical Research Center of Surgery, Mosсow, Russia
Email: nadya-vesy@mail.ru
ORCID iD: 0000-0002-0600-6038
SPIN-code: 9773-1960
Scopus Author ID: 57194481862
Junior Researcher of the Clinical Diagnostic LaboratoryRussian Federation, 117997, Russia, Moscow, B. Serpukhovskaya street, 27
Anna Nikolaevna Kulikova
A.V. Vishnevsky National Medical Research Center of Surgery, Mosсow, Russia
Email: shinshila72@mail.ru
ORCID iD: 0000-0001-5514-5600
SPIN-code: 6535-0560
Doctor of Clinical Diagnostic Laboratory Russian Federation, 117997, Russia, Moscow, B. Serpukhovskaya street, 27
Andrey Mikhailovich Zemskov
N.N. Burdenko Voronezh State Medical University,Voronezh, Russia
Email: zemskov@vsmaburdenko.ru
ORCID iD: 0000-0001-6725-4361
MD, Professor, Head of the Department of Microbiology
Russian Federation, Voronezh, Studentskaya, 10Alexander Eduardovich Bobrovnikov
A.V. Vishnevsky National Medical Research Center of Surgery, Mosсow, Russia;Federal State Budgetary Educational Institution of Further Professional Education "Russian Medical Academy of Continuous Professional Education" of the Ministry of Healthcare of the Russian Federation, Mosсow, Russia
Author for correspondence.
Email: doctorbobr@mail.ru
ORCID iD: 0000-0003-0926-6115
SPIN-code: 4203-1718
Scopus Author ID: 6602689492
MD, Docent, Head of the burn departmentRussian Federation, 117997, Russia, Moscow, B. Serpukhovskaya street, 27
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