Biotechnological Method of Obtaining Nanoparticles of Silver, Cadmium, and Zinc Sulfides. Physico-chemical Characteristics. Creation of Polymeric Nanocomposites


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Abstract

A simple and environmentally safe method for obtaining stable nanoparticles of metal sulfides nanoparticles—NpAg2S, NpCdS, and NpZnS—was developed using different strains of microorganisms in an aqueous solution of metal salts and sulfur sources at the National Research Center Kurchatov Institute—GosNIIgenetika. The concentration of nanoparticles is 1‒4 mg/mL in aqueous suspensions. Using the methods of electron microscopy, spectrofluorimetry, and dynamic light scattering, the main characteristics of biogenic nanoparticles were determined: shape, size distribution, crystal structure, effective diameter, luminescent spectrum, and zeta potential. According to their characteristics, these nanoparticles are referred to quantum dots. It is established that the stability of nanoparticles in aqueous suspensions is due to protein molecules adsorbed on the surface of nanoparticles, which are supplied by cells of microorganisms. Effective immobilization of biogenic nanoparticles on the surface of various polymer supports was carried out. Biogenic nanoparticles along with nanoparticles obtained by physico-chemical methods can be used as fluorophores for imaging of biological processes, also as photocatalysts, solar cells, and for new nanocomposite materials.

About the authors

O. A. Zhuravliova

National Research Center Kurchatov Institute, Research Institute for Genetics and Selection of Industrial Microorganisms; RUDN University

Author for correspondence.
Email: juravlevaol@rambler.ru
Russian Federation, Moscow, 117545; Moscow, 117198

T. A. Voeikova

National Research Center Kurchatov Institute, Research Institute for Genetics and Selection of Industrial Microorganisms

Email: juravlevaol@rambler.ru
Russian Federation, Moscow, 117545

N. V. Bulushova

National Research Center Kurchatov Institute, Research Institute for Genetics and Selection of Industrial Microorganisms

Email: juravlevaol@rambler.ru
Russian Federation, Moscow, 117545

V. P. Veiko

FRC Fundamentals of Biotechnology, Russian Academy of Sciences

Email: juravlevaol@rambler.ru
Russian Federation, Moscow, 119071

T. T. Ismagulova

Moscow State University

Email: juravlevaol@rambler.ru
Russian Federation, Moscow, 119991

T. N. Lupanova

Institute of Gene Biology

Email: juravlevaol@rambler.ru
Russian Federation, Moscow, 119334

S. L. Lobastov

National Research Center “Kurchatov Institute,” IREA

Email: juravlevaol@rambler.ru
Russian Federation, Moscow, 107076

V. M. Retivov

National Research Center “Kurchatov Institute,” IREA

Email: juravlevaol@rambler.ru
Russian Federation, Moscow, 107076

V. G. Debabov

National Research Center Kurchatov Institute, Research Institute for Genetics and Selection of Industrial Microorganisms

Email: juravlevaol@rambler.ru
Russian Federation, Moscow, 117545

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