FEATURES OF THE PHENOTYPE AND NAD(P)-DEPENDENT DEHYDROGENASES ACTIVITY IN NEUTROPHIL BY PATIENTS WITH WIDESPREAD PURULENT PERITONITIS IN PROGNOSIS FOR SEPSIS DEVELOPMENT
- 作者: Savchenko А.А.1,2, Borisov A.G.1,3, Cherdancev D.V.3, Pervova O.V.3, Kudryavtsev I.V.4,5, Gvozdev I.I.6, Moshev A.V.6
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隶属关系:
- Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North
- Siberian Federal University.
- Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky.
- Research Institute of Experimental Medicine
- Pavlov First Saint Petersburg State Medical University.
- Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North.
- 期: 卷 8, 编号 3 (2018)
- 页面: 369-376
- 栏目: SHORT COMMUNICATIONS
- URL: https://journal-vniispk.ru/2220-7619/article/view/118613
- DOI: https://doi.org/10.15789/2220-7619-2018-3-369-376
- ID: 118613
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详细
The aim of the study was to examine the features of phenotype and the levels of NAD(P)-dependent dehydrogenases activity of blood neutrophils in the prognosis of abdominal sepsis development in patients with widespread purulent peritonitis (WPP). 50 patients with WPP of community and hospital origin in the pre-operative period were examined. From the 5th to the 10th day of the postoperative period 35 patients (70%) had developed abdominal sepsis, 15 patients (30%) had absence of complications. As controls 67 respect healthy people were examined. The research blood neutrophils phenotype was performed by f low cytometry using a direct immunofluorescence whole peripheral blood. The levels of surface receptor expression was assessed by the mean fluorescence intensity. The NAD(P)-dependent dehydrogenases activity in the blood neutrophils studied using bioluminescence method. It was established that the inflammatory reaction in patients with WPP is characterized by neutrophilia and changes in the phenotype of blood neutrophils. The markers of the subsequent development of sepsis in WPP are less pronounced (in comparison with the indices of uncomplicated patients), an increase in the number of neutrophils, a decrease in the HLA-DR + -cell count against the background of a high level of neutrophils expressing a high affinity receptor for IgG. The patients without subsequent complications had the number of neutrophils with CD23 receptor expression is increased. Metabolism of neutrophils in patients with WPP is characterized by a decrease in the intensity of plastic processes due to low activity of glucose-6-phosphate dehydrogenase and an imbalance in the activity of the enzymes of the mitochondrial compartment. A feature of the neutrophil metabolism in patients with WPP without subsequent development of sepsis is a high activity of anaerobic lactate dehydrogenase reaction and a decrease in the activity of NADP-dependent decarboxylating malate dehydrogenase. The patients with WPP with subsequent development of sepsis had a high level of NAD-dependent out flow of substrates from the tricarboxylic acid cycle on the amino acid exchange reaction via glutamate dehydrogenase which can affect the activity of aerobic respiration of blood neutrophils. The established differences in the phenotype and activity of enzymes in the blood neutrophils in patients with WPP depending on the subsequent development of sepsis determine the possibility of creating a method for predicting the development of complications and developing immunoactive therapy in the postoperative period of WPP.
作者简介
А. Savchenko
Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North; Siberian Federal University.
Email: fake@neicon.ru
PhD, MD (Medicine), Professor, Head of Laboratory of Molecular Cell Physiology and Pathology; Head of the Department of Physiology.
Krasnoyarsk. 俄罗斯联邦A. Borisov
Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North; Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky.
Email: fake@neicon.ru
hD (Medicine), Leading Researcher, Laboratory of Molecular Cell Physiology and Pathology; Associate Professor, Department of Infectious Diseases.
Krasnoyarsk. 俄罗斯联邦D. Cherdancev
Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky.
Email: fake@neicon.ru
PhD, MD (Medicine), Professor, Head of the Department and Clinic Surgical Diseases named after prof. A.M. Dychno with the course of endoscopy and endosurgery.
Krasnoyarsk. 俄罗斯联邦O. Pervova
Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky.
Email: fake@neicon.ru
PhD, MD (Medicine), Professor of the Department and Clinic Surgical Diseases named after prof. A.M. Dychno with the course of endoscopy and endosurgery.
Krasnoyarsk. 俄罗斯联邦I. Kudryavtsev
Research Institute of Experimental Medicine; Pavlov First Saint Petersburg State Medical University.
编辑信件的主要联系方式.
Email: igorek1981@yandex.ru
PhD (Biology), Senior Researcher, Laboratory of Immunology; Associate Professor, Department of Immunology.
97376, Russian Federation, St. Petersburg, Akademika Pavlova str., 12.
Phone: +7 (812) 234-29-29.
俄罗斯联邦I. Gvozdev
Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North.
Email: fake@neicon.ru
Junior Researcher, Laboratory of Cellular and Molecular Physiology and Pathology.
Krasnoyarsk. 俄罗斯联邦A. Moshev
Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North.
Email: fake@neicon.ru
Junior Researcher, Laboratory of Cellular and Molecular Physiology and Pathology.
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