Neutrophil extracellular traps as markers of infectious and aseptic inflammation
- Authors: Kazimirskii A.N.1, Salmasi J.M.1, Poryadin G.V.1, Panina M.I.1, Kim A.E.1, Rogozhina L.S.1
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Affiliations:
- Pirogov Russian National Research Medical University
- Issue: Vol 14, No 3 (2024)
- Pages: 505-510
- Section: SHORT COMMUNICATIONS
- URL: https://journal-vniispk.ru/2220-7619/article/view/262072
- DOI: https://doi.org/10.15789/2220-7619-NET-16602
- ID: 262072
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Abstract
One of the important problems of medicine is the search for new reliable markers of inflammation that would allow us to determine the type of inflammatory process. The aim of the study was to identify specific markers of infectious and aseptic inflammation based on the analysis of morphological variants of neutrophil extracellular traps (NETs). The study included 26 patients with various nosological forms of inflammation in the abdominal cavity (12 with acute appendicitis, 8 with acute cholecystitis, 6 with a diagnosis of pancreatitis/ pancreonecrosis) after surgery. The study also included 20 patients with post-COVID and a group of 10 volunteers no clinical manifestations of the disease, but at the same time had contacts with flu patients. Neutrophils were isolated using gradient centrifugation. Fluorescence microscopies with the dye SYBR Green (JSC “Evrogen”, Russia) were used for NET visualization and counting. NETs in the morphological form of neutrophils web structure were found in the blood of surgical patients with acute appendicitis who had a favorable course of the postoperative period. In a group of volunteers who were exposed to influenza but did not have symptoms of an infectious disease, we also found neutrophils web structure. In all patients with abdominal inflammation complicated by abdominal abscess, in addition to neutrophil web structure, abnormal forms of NETs were recorded, in particular, NETs in the form of single filaments. In the post-COVID patients an absolute absence of neutrophilis web structure was revealed. In all post-COVID patients, NETs are found only in the form of single filaments. The results of subsequent experiments showed that collagen peptides are inducers of the formation of NETs in the form of single filaments. The detection of neutrophils web structure is a sign of an infectious inflammatory process. NETs in the morphological form of single filaments are markers of aseptic inflammation, as well as a sign of endothelial damage.
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##article.viewOnOriginalSite##About the authors
Alexander N. Kazimirskii
Pirogov Russian National Research Medical University
Author for correspondence.
Email: alnica10@mail.ru
DSc (Biology), Leading Research Associate, Department of Molecular Technologies, Professor, Department of Pathophysiology and Clinical Pathophysiology, Human Biology and Pathology Institute
Russian Federation, 117513, Moscow, Ostrovityanov str., 1J. M. Salmasi
Pirogov Russian National Research Medical University
Email: alnica10@mail.ru
DSc (Medicine), Professor, Head, Department of Pathophysiology and Clinical Pathophysiology, Human Biology and Pathology Institute
Russian Federation, 117513, Moscow, Ostrovityanov str., 1G. V. Poryadin
Pirogov Russian National Research Medical University
Email: alnica10@mail.ru
DSc (Medicine), Corresponding Member, Russian Academy of Sciences, Professor, Department of Pathophysiology and Clinical Pathophysiology, Human Biology and Pathology Institute
Russian Federation, 117513, Moscow, Ostrovityanov str., 1M. I. Panina
Pirogov Russian National Research Medical University
Email: alnica10@mail.ru
DSc (Medicine), Professor, Department of Pathophysiology and Clinical Pathophysiology, Human Biology and Pathology Institute
Russian Federation, 117513, Moscow, Ostrovityanov str., 1A. E. Kim
Pirogov Russian National Research Medical University
Email: alnica10@mail.ru
Assistant Professor, Department of Pathophysiology and Clinical Pathophysiology, Human Biology and Pathology Institute
Russian Federation, 117513, Moscow, Ostrovityanov str., 1L. S. Rogozhina
Pirogov Russian National Research Medical University
Email: alnica10@mail.ru
Assistant Professor, Department of Hospital Surgery No. 1
Russian Federation, 117513, Moscow, Ostrovityanov str., 1References
- Казимирский А.Н., Салмаси Ж.М., Порядин Г.В. Антивирусная система врожденного иммунитета: патогенез и лечение COVID-19 // Вестник РГМУ. 2020. № 5. С. 5–14. [Kazimirskii A.N., Salmasi J.M., Poryadin G.V. Antiviral system of innate immunity: COVID-19 pathogenesis and treatment. Vestnik RGMU = Bulletin of RSMU, 2020, no. 5, pp. 5–13. (In Russ.)]
- Казимирский А.Н., Салмаси Ж.М., Порядин Г.В., Панина М.И. Новые возможности диагностики и исследования патогенеза различных видов воспаления // Патологическая физиология и экспериментальная терапия. 2022. Т. 66, № 2. С. 34–42. [Kazimirskii A.N., Salmasi J.M., Poryadin G.V., Panina M.I. New opportunities for diagnosis and investigation of the pathogenesis of various types of inflammation. Patologicheskaya fiziologiya i eksperimentalnaya terapiya = Pathological Physiology and Experimental Therapy, 2022, vol. 66, no. 2, pp. 34–42. (In Russ.)]
- Салмаси Ж.М., Порядин Г.В., Панина М.И., Ларина В.Н., Рыжих А.А., Стоделова Е.А., Казимирский А.Н. Нейтрофильные и моноцитарные экстраклеточные ловушки в диагностике постковидного синдрома // Вестник РГМУ. 2022. № 6. С. 84–88. [Salmasi J.M., Poryadin G.V., Panina M.I., Larina V.N., Ryzhikh A.A., Stodelova E.A., Kazimirskii A.N. Neutrophil and monocyte extracellular traps in the diagnosis of post-COVID syndrome. Vestnik RGMU = Bulletin of RSMU, 2022, vol. 6, pp. 80–84. (In Russ.)]
- Chrysanthopoulou A., Mitroulis I., Apostolidou E., Arelaki S., Mikroulis D., Konstantinidis T., Sivridis E., Koffa M., Giatromanolaki A., Boumpas D.T., Ritis K., Kambas K. Neutrophil extracellular traps promote differentiation and function of fibroblasts. J. Pathol., 2014, vol. 233, no. 3, pp. 294–307.
- Hidalgo A., Libby P., Soehnlein O., Aramburu I.V., Papayannopoulos V., Silvestre-Roig C. Neutrophil extracellular traps: from physiology to pathology. Cardiovasc. Res., 2022, vol. 118, no. 13, pp. 2737–2753.
- Huang J., Hong W., Wan M., Zheng L. Molecular mechanisms and therapeutic target of NETosis in diseases. MedComm (2020), 2022, vol. 3, no. 3, e162. doi: 10.1002/mco2.162
- Li H., Li J., Bai Z., Yan S., Li J. Collagen-induced DDR1 upregulates CXCL5 to promote neutrophil extracellular traps formation and Treg infiltration in breast cancer. Int. Immunopharmacol., 2023, vol. 120, 110235. doi: 10.1016/j.intimp.2023.110235
- Martins-Goncalves R., Hottz E.D., Bozza P.T. Acute to post-acute COVID-19 thromboinflammation persistence: mechanisms and potential consequences. Curr. Res. Immunol., 2023, vol. 4: 100058. doi: 10.1016/ j.crimmu.2023.100058
- Pieterse E., Rother N., Yanginlar C., Hilbrands L.B., van der Vlag J. Neutrophils discriminate between lipopolysaccharides of different bacterial sources and selectively release neutrophil extracellular traps. Front. Immunol., 2016, vol. 7: 484. doi: 10.3389/fimmu.2016.00484
- Poli V., Zanoni I. Neutrophil intrinsic and extrinsic regulation of NETosis in health and disease. Trends Microbiol., 2023, vol. 31, no. 3, pp. 280–293.
- Roe K. An inflammation classification system using cytokine parameters. Scand. J. Immunol., 2021, vol. 93, no. 2: e12970. doi: 10.1111/sji.12970
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