Studying the bactericidal effect of commercial disinfectants on Brucella
- Authors: Zharinova N.V.1, Serdyuk N.S.1, Zhilchenko E.B.2, Karapetyan M.G.2, Belozyorova O.N.2
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Affiliations:
- Stavropol Plague Control Research Institute
- Federal state healthcare institution of Stavropol anti-plague Institute of Rospotrebnadzor
- Issue: Vol 14, No 5 (2024)
- Pages: 1017-1020
- Section: SHORT COMMUNICATIONS
- URL: https://journal-vniispk.ru/2220-7619/article/view/284838
- DOI: https://doi.org/10.15789/2220-7619-STB-17628
- ID: 284838
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Abstract
Pathogens, particularly Brucella spp., pose a serious threat to human and animal health. In the complex of anti-epidemic and sanitary-hygienic measures aimed at preventing infectious diseases, chemical disinfection plays an important role. This disinfection method is one of the effective, reliable and common ways to combat pathogenic biological agents. An important role is played by modern quaternary ammonium compounds (QAC)-based disinfectants. In relation to chemical disinfectants, the causative agent of brucellosis belongs to the group of low-resistant microorganisms. Solutions of sublimate, creolin, phenol, sulfuric, hydrochloric, nitric and acetic acids, formalin, chloramine, hydrogen peroxide, solutions of disinfectants based on QAC, triamine and polyhexamethylene guanidine (PHMG) have pronounced bactericidal activity against brucellae. However, the instructions for commercial disinfectants note a bactericidal activity against pathogens of particularly dangerous infections (plague, tularemia, cholera and anthrax), but do not indicate antimicrobial properties against Brucella. The purpose of the work was to determine the antimicrobial properties of five commercial disinfectants “Alfadez Forte”, “Aminaz-Plus”, “Veltolen”, “Desarin” and “Laina-med”, used in the disinfection of objects with especially dangerous infections of bacterial etiology. The studies were carried out using the suspension method, according to the manual “Methods of laboratory research and testing of disinfectants to assess their effectiveness and safety”. Three vaccine strains B. melitensis Rev-1, B. abortus 19 BA, B. suis 61 were used as test microbes. As a result of the studies, it was found that commercial disinfectants “Alfadez Forte”, “Aminaz-Plus”, “Veltolen”, “Desarin” and “Lina-med” in the concentrations proposed by the manufacturers in the instructions for use when working with particularly dangerous infections (plague, tularemia, cholera) have bactericidal properties relative to vaccine strains.
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##article.viewOnOriginalSite##About the authors
N. V. Zharinova
Stavropol Plague Control Research Institute
Email: manya.karapetyan.ncfu@gmail.com
PhD (Biology), Senior Researcher, Laboratory “Сollection of Pathogenic Microorganisms”
Russian Federation, StavropolN. S. Serdyuk
Stavropol Plague Control Research Institute
Email: manya.karapetyan.ncfu@gmail.com
PhD (Biology), Senior Researcher, Laboratory “Сollection of Pathogenic Microorganisms”
Russian Federation, StavropolE. B. Zhilchenko
Federal state healthcare institution of Stavropol anti-plague Institute of Rospotrebnadzor
Email: manya.karapetyan.ncfu@gmail.com
PhD (Biology)., head of the laboratory of "Collection of pathogenic microorganisms"
Russian Federation, StavropolManya G. Karapetyan
Federal state healthcare institution of Stavropol anti-plague Institute of Rospotrebnadzor
Author for correspondence.
Email: manya.karapetyan.ncfu@gmail.com
Biologist, Laboratory “Сollection of Pathogenic Microorganisms”
Russian Federation, StavropolO. N. Belozyorova
Federal state healthcare institution of Stavropol anti-plague Institute of Rospotrebnadzor
Email: manya.karapetyan.ncfu@gmail.com
Biologist, Laboratory “Сollection of Pathogenic Microorganisms”
Russian Federation, StavropolReferences
- Афиногенов Г.Е., Краснова М.В., Афиногенова А.Г. Сравнение методов оценки эффективности дезинфектантов и антисептиков // Дезинфекционное дело. 2008. № 5. С. 40–44. [Afinogenov G.E., Krasnova M.V., Afinogenova A.G. Comparison of methods for evaluating the effectiveness of disinfectants and antiseptics. Dezinfektsionnoe delo = Disinfection Affairs, 2008, no. 4, pp. 40–44. (In Russ.)]
- Воинцева И.И., Гембицкий П.А. Полигуанидины — дезинфекционные средства и полифункциональные добавки в композиционные материалы. М.: ЛКМ-пресс, 2009. 303 с. [Vointseva I.I., Gembitskiy P.A. Polyguanidines — disinfectants and multifunctional additives in composite materials. Moscow: LKM-Press, 2009. 303 p. (In Russ.)]
- Очиров О.С., Бурасова Е.Г., Стельмах С.А., Григорьева М.Н., Окладникова В.О., Могнонов Д.М. Антимикробная активность производных полигексаметиленгуанидина гидрохлорида по отношению к мультирезистентным штаммам микроорганизмов // Инфекция и иммунитет. 2022. Т. 12, № 1. С. 193–196. [Ochirov O.S., Burasova E.G., Stelmakh S.A., Grigorieva M.N., Okladnikova V.O., Mognonov D.M. Antimicrobial activity of polyhexamethylene guanidine hydrochloride derivatives in relation to multi-resistant strains of microorganisms. Infektsiya i immunitet = Russian Journal of Infection and Immunity, 2022, vol. 12, no. 1, pp. 193–196. (In Russ.)] doi: 10.15789/2220-7619-AAO-1751
- Палий А.П., Синица Е.В., Дубин Р.А., Ведмидь А.В., Палий А.П. Изучение показателей бактериоцидности дезинфектантов серии «Неодез» // Вестник Алтайского государственного аграрного университета. 2016. № 6 (140). С. 108–111. [Paliy A.P., Sinitsa E.V., Dubin R.A., Vedmid A.V., Paliy A.P. The study of bacteriocidicity indicators of disinfectants of the Neodez series. Vestnik Altaiskogo gosudarstvennogo agrarnogo universiteta = Bulletin of Altai State Agricultural University, 2016, no. 6 (140), pp. 108–111. (In Russ.)]
- Пономаренко Д.Г., Скударева О.Н., Хачатурова А.А., Лукашевич Д.Е., Жаринова И.В., Даурова А.В., Германова А.Н., Логвиненко О.В., Ракитина Е.Л., Костюченко М.В., Манин Е.А., Малецкая О.В., Куличенко А.Н. Бруцеллез: тенденции развития ситуации в мире и прогноз на 2022 г. в Российской Федерации // Проблемы особо опасных инфекций. 2022. № 2. С. 36–45. [Ponomarenko D.G., Skudareva O.N., Khachaturova A.A., Lukashevich D.E., Zharinova I.V., Daurova A.V., Germanova A.N., Logvinenko O.V., Rakitina E.L., Kostyuchenko M.V., Manin E.A., Maletskaya O.V., Kulichenko A.N. Brucellosis: trends in the development of the situation in the world and forecast for 2022 in the Russian Federation. Problemy osobo opasnykh infektsiy = Problems of Particularly Dangerous Infections, 2022, no. 2, pp. 36–45. (In Russ.)] doi: 10.21055/0370-1069-2022-2-36-45
- Спиридонов В.А., Андрус В.Н., Елизаров В.В. Эффективность некоторых дезинфицирующих средств при обеззараживании поверхностей и изделий медицинского назначения, контаминированных потенциально опасными биологическими агентами бактериальной природы категории А // Проблемы особо опасных инфекций. 2009. № 102. С. 36–45. [Spiridonov V.A., Andrus V.N., Elizarov V.V. Effectiveness of some disinfectants in disinfection of surfaces and medical devices contaminated with potentially dangerous biological agents of bacterial nature of category A. Problemy osobo opasnykh infektsiy = Problems of Particularly Dangerous Infections, 2009, no. 102, pp. 36–45. (In Russ.)] doi: 10.21055/0370-1069-2009-4(102)-37-39
- Шкарин В.В. Саперкин Н.В., Ковалишена О.В., Благонравова А.С., Широкова И.Ю., Кулюкина А.А. Региональный мониторинг устойчивости микроорганизмов к дезинфектанам: итоги и перспективы // Медицинский альманах. 2012. Т. 3, № 22. С. 122–125. [Shkarin V.V., Saperkin N.V., Kovalishena O.V., Blagonravova A.S., Shirokova I.Yu., Kulyukina A.A. The regional monitoring of microorganisms resistance to disinfectants: results and perspectives. Meditsinskiy almanakh = Medical Almanac, 2012, vol. 3, no. 22, pp. 122–125. (In Russ.)]
- Albert M., Feiertag P., Hayn G. Structure-activity relationships of oligoguanidiness influence of counterion, diamine, and average molecular weight on biocidal activities. Biomacromolecules, 2003, vol. 4, pp. 1811–1817. doi: 10.1021/bm0342180
- Cloete T.E. Resistance mechanisms of bacteria to antimicrobial compounds. International Biodeterioration & Biodegradation, 2003, vol. 51, iss. 4, pp. 277–282. doi: 10.1016/S0964-8305(03)00042-8
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