Modern research on molecular identification of Fusarium solani

Мұқаба

Дәйексөз келтіру

Толық мәтін

Аннотация

Background. Fungi of the genus Fusarium are representatives of a biologically heterogeneous group, which includes saprophytes and facultative parasites. Traditional morphological methods for identifying representatives of this genus are often not reliable enough due to the variability of features and the complexity of the Fusarium solani species complex. Materials and methods. The review analyzes modern molecular genetic methods based on the analysis of sequences of markers such as ITS, TEF-1α, RPB2, β-tubulin, NIR, PHO and the SIX gene, which have significantly increased the accuracy of diagnosis, making it possible to distinguish closely related species, races and specialized forms. Recently, special attention has been paid to the advantages of the multilocus approach, which is recognized as the “gold standard” for the identification and taxonomic analysis of Fusarium solani representatives. This research also examines the potential and limitations of next-generation sequencing (NGS), including WGS, tNGS, and mNGS, for comprehensive genomic analysis and diagnostics of pathogens. Results and conclusions. It is noted that the integration of phenotypic and molecular data, as well as the development of genomic technologies, open up new prospects for standardization and increased accuracy of Fusarium solani identification at the species and intraspecific levels.

Негізгі сөздер

Авторлар туралы

Farkhod Rajapov

Center of genomics and bioinformatics Academy of Sciences of the Republic of Uzbekistan

Хат алмасуға жауапты Автор.
Email: farhod.radjapov@yandex.ru

Senior researcher of the laboratory of plant genomics resistance

(2 Universitetskaya street, village Salar, Tashkent region, Uzbekistan)

Ilkhom Salakhutdinov

Center of genomics and bioinformatics Academy of Sciences of the Republic of Uzbekistan

Email: ilkhom.salakhutdinov@genomics.uz

Candidate of biological sciences, head of the laboratory of plant genomics resistance

(2 Universitetskaya street, village Salar, Tashkent region, Uzbekistan)

Venera Kamburova

Center of genomics and bioinformatics Academy of Sciences of the Republic of Uzbekistan

Email: kamburova.v@genomics.uz

Doctor of biological sciences, deputy director for science

(2 Universitetskaya street, village Salar, Tashkent region, Uzbekistan)

Elvira Latypova

Penza State Agrarian University

Email: latypova.e.a@pgau.ru

Candidate of biological sciences, associate professor of the sub-department of biology, biological technologies and veterinary and sanitary expertise

(30 Botanicheskaya street, Penza, Russia)

Zabardast Buriev

Center of genomics and bioinformatics Academy of Sciences of the Republic of Uzbekistan

Email: zburiev@genomics.uz

Doctor of biological sciences, professor, director

(2 Universitetskaya street, village Salar, Tashkent region, Uzbekistan)

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