Effect of low positive temperature on the antioxidant system formation in de-etiolated and etiolated Amaranthus tricolor L. seedlings grown from seeds treated with growth regulators
- Authors: Gins E.M.1
-
Affiliations:
- Russian Potato Research Center
- Issue: Vol 18, No 4 (2023): Pesticides. Looking to the future
- Pages: 520-530
- Section: Crop production
- URL: https://journal-vniispk.ru/2312-797X/article/view/315736
- DOI: https://doi.org/10.22363/2312-797X-2023-18-4-520-530
- EDN: https://elibrary.ru/LJLAEU
- ID: 315736
Cite item
Abstract
In the Non-chernozem zone of Russia, the recurrent spring cold up to 1-2 °C can cause damage and death of thermophilic amaranth seedlings. Pre-sowing treatment of seeds with growth regulators is an inexpensive and effective method to reduce the negative effect of hypothermia on seed germination. The aim of the research was to study the effect of low-temperature stress on etiolated and de-etiolated seedlings of amaranth cv. ‘Valentina’ ( A. tricolor L.) grown from seeds treated with growth stimulants. Seeds were pretreated with aqueous solutions of Albit (1 g/L), hydrogen peroxide (H2O2) - 5 mM and succinic acid (ScA) - 500 mg/L. Seeds were germinated in peat pots at 23 ± 2 °C (T23) for 7 days. On the 7th day, peat pots with seedlings grown in the light and in the dark were moved to thermostat at 2.0 ± 0.5 °C (T2) for 8 hours. Determination of the amount of amaranthine, chlorophylls and carotenoids were carried out according to generally accepted methods. Pretreatment of seeds with the growth regulators Albit, H2O2, and ScA increased hypocotyl length, root length, and biomass of light and etiolated seedlings. Low positive temperatures increased the content of amaranthine and carotenoids but reduced the content of chlorophylls. It was shown that all used growth regulators - H2O2, Albit and ScA trigger or at least maintain the system of antioxidant protection in light and etiolated seedlings of amaranth cv. ‘Valentina’ under low positive temperatures.
About the authors
Ekaterina M. Gins
Russian Potato Research Center
Author for correspondence.
Email: katya.888888@yandex.ru
ORCID iD: 0000-0002-5685-6305
PhD student, Junior Researcher
23 Lorkha st., Lyubertsy district, Kraskovo vil., Moscow region, 140051, Russian FederationReferences
- Sanghera GS, Wani SH, Hussain W, Singh NB. Engineering cold stress tolerance in crop plants. Current Genomics. 2011;12(1)30-43. doi: 10.2174/138920211794520178
- Park AR, Kim J, Kim B, Ha A, Son JY, Song CW, et al. Exogenous Bio-B ased 2,3-Butanediols Enhanced Abiotic Stress Tolerance of Tomato and Turfgrass under Drought or Chilling Stress. J Microbiol Biotechnol. 2022;32(5):582-593. doi: 10.4014/jmb.2201.01025
- Sarker U, Hossain MN, Iqbal MA, Oba S. Bioactive components and radical scavenging activity in selected advance lines of salt-tolerant vegetable amaranth. Front Nutr. 2020;7:587257. doi: 10.3389/fnut.2020.587257
- Hussain HA, Hussain S, Khaliq A, Ashraf U, Anjum SA, Men S, et al. Chilling and drought stresses in crop plants: implications, cross talk, and potential management opportunities. Front Plant Sci. 2018;9:393; doi: 10.3389/fpls.2018.00393
- Mittler R. Oxidative stress, antioxidants and stress tolerance. Trend Plant Sci. 2002;7(9):405-410. doi: 10.1016/S1360-1385(02)02312-9
- Gill SS, Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem. 2010;48(12):909-930. doi: 10.1016/j.plaphy.2010.08.016
- Rhaman MS, Imran S, Rauf F, Khatun M, Baskin CC, Murata Y, et al. Seed priming with phytohormones: an effective approach for the mitigation of abiotic stress. Plants. 2021;10(1):37. doi: 10.3390/plants10010037
- Jisha KC, Vijayakumari K, Puthur JT. Seed priming for abiotic stress tolerance: an overview. Acta Physiol Plant. 2013;35:1381-1396. doi: 10.1007/s11738-012-1186-5
- Pandey P, Ramegowda V, Senthil-K umar M. Shared and unique responses of plants to multiple individual stresses and stress combinations: physiological and molecular mechanisms. Front Plant Sci. 2015;6:723. doi: 10.3389/fpls.2015.00723
- Lichtenthaler HK. Chlorophylls and carotenoids - pigments of photosynthetic biomembranes. Methods in Enzymology. 1987;148:350-382. doi: 10.1016/0076-6879(87)48036-1
- Gins MS, Gins VK, Kononkov PF. Change in the biochemical composition of amaranth leaves during selection for increased amaranthine content. Applied Biochemistry and Microbiology. 2002;38(5):474-479. doi: 10.1023/A:1019980821313
- Fox J, Leanage A. R and the Journal of Statistical Software. J Stat Softw. 2016;73(2):1-13. doi: 10.18637/jss.v073.i02
- Li S, Jiang H, Wang J, Wang Y, Pan S, Tian H, et al. Responses of plant growth, physiological, gas exchange parameters of super and non-super rice to rhizosphere temperature at the tillering stage. Sci Rep. 2019;9(1):10618. doi: 10.1038/s41598-019-47031-9
- Tewari AK, Tripathy BC. Temperature-stress-induced impairment of chlorophyll biosynthetic reactions in cucumber and wheat. Plant Physiol. 1998;117(3):851-858. doi: 10.1104/pp.117.3.851
- Wittayathanarattana T, Wanichananan P, Supaibulwatana K, Goto E. A short-term cooling of root-zone temperature increases bioactive compounds in baby leaf Amaranthus tricolor L. Front Plant Sci. 2022;13:944716. doi: 10.3389/fpls.2022.944716
- Deng XP, Cheng YJ, Wu XB, Kwak SS, Chen W, Eneji AE. Exogenous hydrogen peroxide positively influences root growth and metabolism in leaves of sweet potato seedlings. Aust J Crop Sci. 2012;6(11):1572-1578.
- Baroli I, Niyogi KK. Molecular genetics of xanthophyll-dependentphotoprotection in green algae and plants. Philos Trans R Soc Lond. BBiol Sci. 2000;355(1402):1385-1394. doi: 10.1098/rstb.2000.0700
- Rodriéguez-C oncepcioén M, Foreés O, Martiénez-Garciéa JF, Gonzaélez V, Phillips MA, Ferrer A, et al. Distinct light-m ediated pathways regulate the biosynthesis and exchange of isoprenoid precursors during Arabidopsis seedling development. The Plant Cell. 2004;16(1):144-156. doi: 10.1105/tpc.016204
- Nakashima T, Araki T, Ueno O. Photoprotective function of foliar betacyanin in leaves of Amaranthus Cruentus under drought stress. In: Photosynthesis Research for Food, Fuel and the Future: 15th International Conference on Photosynthesis. Berlin, Heidelberg: Springer; 2013. p.485-488. doi: 10.1007/978-3-642-3203 4-7_102
- Stanley L, Yuan YW. Transcriptional regulation of carotenoid biosynthesis in plants: so many regulators, so little consensus. Front Plant Sci. 2019;10:1017. doi: 10.3389/fpls.2019.01017
- Stahl W, Sies H. Antioxidant activity of carotenoids. Mol Asp Med. 2003;24(6):345-351. doi: 10.1016/S0098-2997(03)00030-X
Supplementary files
