Effects of nitric oxide associated with expression of some genes at embryonic stage of avian development


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Abstract

Data obtained by various researchers indicate the effect of nitric oxide (NO) on expression of some genes, in particular, the genes associated with myogenesis in birds. But until now it has not been possible to quantify this effect and possibility of its use, since the control of content of NO metabolites in tissues presented a methodological difficulty. The purpose of this research was to find out relationship between the NO content in tissues of avian embryos and the expression of genes responsible for myogenesis at various stages of embryogenesis. We used a highly sensitive and highly specifi enzyme sensor to determine the content of the main NO metabolites in tissues. Gene expression was determined by PCR-RT. The relationship between the NO content in tissues and the expression of 7 genes involved in the process of myogenesis was studied in chicken and quail embryos. There are the genes of myocyte proliferation factor 2c (mef 2c), myogenic differentiation 1 (MyoD1), myogenesis factor 5 (myf 5), myosin (mhy 1), myogenin (myog), somatostatin (MSTN), gonadotropin hormone (GHR). Blocking the synthesis of NO, which leads to a decrease in deposited NO in tissues by 50…70%, results in a change in expression of most of the studied genes. Basically, there was a decrease in gene expression, in particular, myostatin (MSTN), which is responsible for suppressing growth and differentiation of muscle tissue. Thus, nitric oxide in an avian embryo can primarily play the role of regulator of muscle tissue growth, which is important for fast-growing breeds since myoblast proliferation occurs at embryonic stage of development. Regulation can be carried out by activating the mechanisms of oxidation of NO to nitrate which occurs in the embryos of fast-growing breeds.

About the authors

Vladimir Y. Titov

Moscow State Academy of Veterinary Medicine and Biotechnology - MVA named after K.I. Skryabin; Russian Research and Technological Poultry Institute

Author for correspondence.
Email: vtitov43@yandex.ru
ORCID iD: 0000-0002-2639-7435
SPIN-code: 3912-0315

Doctor of Biological Sciences, Professor of Department of Radiobiology and Biophysics, Moscow State Academy of Veterinary Medicine and Biotechnology - MVA named after K.I. Skryabin; Chief Researcher, Russian Research and Technological Institute of Poultry Farming

23 Akademika Scriabina st., Moscow, 109472, Russian Federation; 10 Ptitsegradskaya st., Sergiev Posad, Moscow region, 141311, Russian Federation

Ivan I. Kochish

Moscow State Academy of Veterinary Medicine and Biotechnology - MVA named after K.I. Skryabin

Email: kochish.I@mail.ru
ORCID iD: 0000-0001-8892-9858
SPIN-code: 9333-9995

Academician of the Russian Academy of Sciences, Doctor of Agricultural Sciences, Head of the Department of Animal Hygiene and Poultry Breeding named after A.K. Danilova

23 Akademika Scriabina st., Moscow, 109472, Russian Federation

Olga V. Myasnikova

Moscow State Academy of Veterinary Medicine and Biotechnology - MVA named after K.I. Skryabin

Email: omyasnikova71@gmail.com
ORCID iD: 0000-0002-9869-0876
SPIN-code: 1466-8393

Candidate of Agricultural Sciences, Associate Professor, Department of Animal Hygiene and Poultry Breeding named after A.K. Danilova

23 Akademika Scriabina st., Moscow, 109472, Russian Federation

Anna M. Dolgorukova

Russian Research and Technological Poultry Institute

Email: anna.dolg@mail.ru
ORCID iD: 0000-0002-9958-8777
SPIN-code: 7630-3146

Candidate of Biological Sciences, Head of the Incubation Department

10 Ptitsegradskaya st., Sergiev Posad, Moscow region, 141311, Russian Federation

Maria A. Zaytseva

Moscow State Academy of Veterinary Medicine and Biotechnology - MVA named after K.I. Skryabin

Email: may-zay@yandex.ru
student, Faculty of Biotechnology and Ecology 23 Akademika Scriabina st., Moscow, 109472, Russian Federation

Pyotr S. Romanov

Moscow State Academy of Veterinary Medicine and Biotechnology - MVA named after K.I. Skryabin

Email: romanovpeter1367@yandex.ru
student, Faculty of Biotechnology and Ecology 23 Akademika Scriabina st., Moscow, 109472, Russian Federation

Harutyun R. Vardanyan

Scientific Center for Food Safety Risk Assessment and Analysis

Email: harvard3@yandex.ru
ORCID iD: 0009-0002-1050-8433

Candidate of Biological Sciences, Researcher

107 Masisi Shosse, bldg. 2, Yerevan, 0071, Republic of Armenia

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