Migration, proliferation and cell death of regenerating liver macrophages in an experimental model
- 作者: Grinberg M.V.1, Lokhonina A.V.2, Vishnyakova P.A.2, Makarov A.V.3, Kananykhina E.Y.4, Eremina I.Z.1, Glinkina V.V.3, Elchaninov A.V.4, Fatkhudinov T.K.4
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隶属关系:
- RUDN University
- National Medical Research Center for Obstetrics, Gynecology and Perinatology Named After Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation
- Pirogov Russian National Research Medical University, Ministry of Healthcare of the Russian Federation
- Avtsyn Research Institute of Human Morphology of Federal state budgetary scientific institution «Petrovsky National Research Centre of Surgery»
- 期: 卷 27, 编号 4 (2023): PHYSIOLOGY. EXPERIMENTAL PHYSIOLOGY
- 页面: 449-458
- 栏目: PHYSIOLOGY. EXPERIMENTAL PHYSIOLOGY
- URL: https://journal-vniispk.ru/2313-0245/article/view/319695
- DOI: https://doi.org/10.22363/2313-0245-2023-27-4-449-458
- EDN: https://elibrary.ru/JDEJXS
- ID: 319695
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Relevance . Macrophages are the leading regulatory cell-lineage taking part in reparative processes in mammals, and the liver is no exception. The ratio of monocyte migration, proliferation and death of macrophages during liver regeneration requires further studies. The aim was to quantify the intensity of monocyte migration, cell proliferation and apoptosis of resident liver macrophages after its 70 % resection in a mouse model. Materials and Methods. We performed 70 % liver resection in sexually mature male BalbC mice. Cells of liver monocyte-macrophage system were obtained by magnetic sorting by marker F4/80. The immunophenotype of the isolated cells was further studied by cytofluorimetry, the level of proliferation and cell death, the content of cyclins and P53 was determined by western blot. Results and Discussion . It was found that after partial hepatectomy there is a marked migration of monocytes/macrophages positive for Ly6C and CD11b markers to the liver, the migration process starts already in the first day after the operation. On the same terms there is a rise in proliferative activity of macrophages, established by Ki67 marker, the peak of proliferation - 3 days after partial hepatectomy. A significant increase in the number of dying macrophages was found early after liver resection. Conclusion . The obtained data indicate that liver regeneration in mammals on the model in mice is accompanied by proliferation migration and cell death of macrophages. Taking into account the immunophenotype of macrophages, we can conclude that Ly6C+ blood monocytes migrate to the liver, and resident macrophages participate in proliferation. The obtained data confirm the universality of the course of reparative processes in mammals.
作者简介
Maria Grinberg
RUDN University
Email: elchandrey@yandex.ru
ORCID iD: 0000-0002-9159-4232
SPIN 代码: 6260-1863
Moscow, Russian Federation
Anastasia Lokhonina
National Medical Research Center for Obstetrics, Gynecology and Perinatology Named After Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation
Email: elchandrey@yandex.ru
ORCID iD: 0000-0001-8077-2307
SPIN 代码: 4521-2250
Moscow, Russian Federation
Polina Vishnyakova
National Medical Research Center for Obstetrics, Gynecology and Perinatology Named After Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation
Email: elchandrey@yandex.ru
ORCID iD: 0000-0001-8650-8240
SPIN 代码: 3406-3866
Moscow, Russian Federation
Andrey Makarov
Pirogov Russian National Research Medical University, Ministry of Healthcare of the Russian Federation
Email: elchandrey@yandex.ru
ORCID iD: 0000-0003-2133-2293
SPIN 代码: 3534-3764
Moscow, Russian Federation
Eugenia Kananykhina
Avtsyn Research Institute of Human Morphology of Federal state budgetary scientific institution «Petrovsky National Research Centre of Surgery»
Email: elchandrey@yandex.ru
ORCID iD: 0000-0002-9779-2918
SPIN 代码: 8256-5754
Moscow, Russian Federation
Irina Eremina
RUDN University
Email: elchandrey@yandex.ru
ORCID iD: 0000-0001-5444-9231
SPIN 代码: 5819-6159
Moscow, Russian Federation
Valeria Glinkina
Pirogov Russian National Research Medical University, Ministry of Healthcare of the Russian Federation
Email: elchandrey@yandex.ru
ORCID iD: 0000-0001-8708-6940
SPIN 代码: 4425-5052
Moscow, Russian Federation
Andrey Elchaninov
Avtsyn Research Institute of Human Morphology of Federal state budgetary scientific institution «Petrovsky National Research Centre of Surgery»
编辑信件的主要联系方式.
Email: elchandrey@yandex.ru
ORCID iD: 0000-0002-2392-4439
SPIN 代码: 5160-9029
Moscow, Russian Federation
Timur Fatkhudinov
Avtsyn Research Institute of Human Morphology of Federal state budgetary scientific institution «Petrovsky National Research Centre of Surgery»
Email: elchandrey@yandex.ru
ORCID iD: 0000-0002-6498-5764
SPIN 代码: 7919-8430
Moscow, Russian Federation
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