Email this article Identification of betulin in the bark of aspen by liquid chromatography
- Authors: Ershik V.A.1, Mushkina V.V.1, Fisiuk A.Y.1
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Affiliations:
- Belarusian State Medical University
- Issue: Vol 25, No 3 (2025)
- Pages: 52-58
- Section: PHARMACEUTICAL CHEMISTRY, PHARMACOGNOSY
- URL: https://journal-vniispk.ru/2410-3764/article/view/315115
- DOI: https://doi.org/10.35693/AVP680101
- ID: 315115
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Abstract
Aim – screening of biologically active substances of common aspen bark, detection and identification of betulin in the bark of aspen by liquid chromatography.
Material and methods. The object of the study is dried and crushed samples of aspen bark. Qualitative analysis of coumarins, flavonoids, phenolic compounds and saponins was carried out using qualitative chemical reactions for the indicated groups of biologically active substances. Alcohol extraction and purified betulin fraction were used for chromatography. Chromatography was performed using an UltiMate 3000 liquid chromatograph. To separate the components, a column with a length of 250 mm and an inner diameter of 4.6 mm filled with octadecylsilyl silica gel for chromatography with a particle size of 5 microns was used. A mixture of acetonitrile-water 90:10 (v/v) in the isocratic mode was used as the mobile phase. Detection was performed at a wavelength of 206 nm.
Results. Using the method of qualitative chemical analysis, coumarins, flavonoids, phenolic compounds and triterpene saponins were found in samples of common aspen bark. Peaks with a retention time of about 7.5 minutes with similar spectral characteristics were found on all chromatograms. This allows us to conclude that the alcohol extraction of aspen bark and the isolated, purified fraction contain significant amounts of betulin. The betulin content in the alcohol extraction (n=3) was 0.77±0.04%.
Conclusion. 1. Using the method of qualitative chemical analysis, coumarins, flavonoids, triterpene saponins and phenolic compounds were found in samples of common aspen bark. 2. Betulin was detected and identified by liquid chromatography in the bark of aspen. 3. Betulin is recommended as an analytical marker in the identification of aspen bark by liquid chromatography. 4. The prospects of using liquid chromatography for the identification and assessment of the degree of purification of betulin during its isolation from the bark of aspen are shown.
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##article.viewOnOriginalSite##About the authors
Volha A. Ershik
Belarusian State Medical University
Author for correspondence.
Email: Olgavgmugnoz@mail.ru
ORCID iD: 0009-0008-9977-7464
Cand. Sci. (Pharmacy), Associate professor, Associate professor of the Department of Pharmacy Organization with a course of advanced training and retraining
Belarus, MinskVolha V. Mushkina
Belarusian State Medical University
Email: olga7081@tut.by
ORCID iD: 0000-0002-3397-1220
Cand. Sci. (Pharmacy), Associate professor, Head of the Department of Pharmacy Organization with a course of advanced training and retraining
Belarus, MinskAleksandr Yu. Fisiuk
Belarusian State Medical University
Email: a.fisiuk@yandex.bu
ORCID iD: 0009-0007-6220-514X
trainee teacher of the Department of Pharmacy Organization with a course of advanced training and retraining
Belarus, MinskReferences
- Skurydina ES, Vasil’yeva NYu, Kuznetsova SA, et al. Development of the one-step method for producing allobetulin from birch bark and study of its antioxidant activity. Chemistry of plant raw material. 2023;3:243-252. [Скурыдина Е.С., Васильева Н.Ю., Кузнецова С.А., и др. Разработка одностадийного способа получения аллобетулина из бересты коры березы и изучение его антиоксидантной активности. Химия растительного сырья. 2023;3:243-252]. doi: 10.14258/jcprm.20230313179
- Frolkova KA. Betulin as an antioxsdant. Forcipe. 2023;6(2):218. (In Russ.). [Фролкова К.А. Бетулин как антиоксидант. Forcipe. 2023;6(2):218].
- Averyanova EV, Shkolnikova MN, Chugunova OV. Antioxidant Properties of Triterpenoids in Fat-Containing Products. Food Processing: Techniques and Technology. 2022;52(2):233-243. [Аверьянова Е.В., Школьникова М.Н., Чугунова О.В. Исследование антиоксидантных свойств тритерпеноидов в составе жиросодержащих продуктов. Техника и технология пищевых производств. 2022;52(2):233-243]. doi: 10.21603/2074-9414-2022-2-2358
- Kuznetsova SA, Skvortsova GP, Maliar YuN, et al. Extraction bеtulin from birch bark and study of its physicochemical and pharmacological properties. Chemistry of plant raw material. 2013;2:93-100. [Кузнецова С.А., Скворцова Г.П., Маляр Ю.Н., и др. Выделение бетулина из бересты березы и изучение его физико-химических и фармакологических свойств. Химия растительного сырья. 2013;2:93-100]. doi: 10.14258/jcprm.1302093
- Amiri S, Dastghaib S, Ahmadi M, et al. Betulin and its derivatives as novel compounds with different pharmacological effects. Biotechnology Advanced. 2020;38:107409. doi: 10.1016/j.biotechadv.2019.06.008
- Sergeev DV. Clinical and experimental evaluation of anti-inflammatory properties of betulin (In Russ.). [Сергеев Д.В. Клинико-экспериментальная оценка противовоспалительных свойств бетулина]. URL: https://medical-diss.com/medicina/kliniko-eksperimentalnaya-otsenka-protivovospalitelnyh-svoystv-betulina (02 May 2025)
- Vorob’eva OA, Mel’nikova NB. Development and validation of methods for analyzing the components of the pharmaceutical composition betulin and thymol in pumpkin seed Cucurbita pepo oil. Mezhdunarodnyi zhurnal prikladnykh i fundamental’nykh issledovanii. 2015;10(2):295-303. [Воробьева О.А., Мельникова Н.Б. Разработка и валидация методов анализа компонентов фармацевтической композиции бетулина и тимола в масле семян тыквы Cucurbita pepo. Международный журнал прикладных и фундаментальных исследований. 2015;10(2):295-303]. URL: https://applied-research.ru/ru/article/view?id=7487
- Lebedeva RA, Malygina DS. Identification and quantitative determination of betulin diacetate as a potential pharmaceutical substance. Fundamental and applied research: problems and results. 2016;24:155-160. [Лебедева Р.А., Малыгина Д.С. Идентификация и количественное определение диацетата бетулина как потенциальной фармацевтической субстанции. Фундаментальные и прикладные исследования: проблемы и результаты. 2016;24:155-160]. URL: https://cyberleninka.ru/article/n/identifikatsiya-i-kolichestvennoe-opredelenie-diatsetata-betulina-kak-potentsialnoy-farmatsevticheskoy-substantsii (26.05.2025)
- Levdansky VA, Levdansky AV. Extraction of betulin by aliphatic alcohols c3-c4 from an external layer of a birch bark hydrolyzed in aqueous alkaline solution. Chemistry of plant raw material. 2014;1:131-137. [Левданский В.А., Левданский А.В. Экстракция бетулина алифатическими спиртами С3-C4 из бересты березы, гидролизованной в водном растворе щелочи. Химия растительного сырья. 2014;1:131-137]. doi: 10.14258/jcprm.1401131
- Kasenov RZ, Demets OV, Kartbaeva GT, et al. Study of the quantitative yield of betulin from the Kyrgyz birch and synthesis of its phosphorylated derivative. Bulletin of Karaganda University. 2019;3(95). [Касенов Р.З., Демец О.В., Картбаева Г.Т., и др. Исследование количественного выхода бетулина из березы киргизской и синтез его фосфорилированного производного. Вестник Карагандинского университета. 2019;3(95)]. doi: 10.31489/2019bmg3/13-19
- Ayvazyan AA, Bozova AP, Nagaeva KA, et al. Influence of production methods and particle sizes of raw materials on the yield and purity of betulin. In: Waste processing technologies to obtain new products. 2022:141-143. (In Russ.). [Айвазян А.А., Бозова А.П., Нагаева К.А., и др. Влияние способов получения и размеров частиц сырья на выход и чистоту бетулина. В сб.: Технологии переработки отходов с получением новой продукции. 2022:141-143].
- Demets OV, Takibayeva AT, Kassenov RZ, et. al. Methods of Betulin Extraction from Birch Bark. Molecules. 2022:27(11):3621. doi: 10.3390/molecules27113621
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