Email this article Development of the method for spectrophotometric quantitative determination of the total flavonoids in Bupleurum multinerve herb
- Authors: Petukhova S.A.1, Posokhina A.A.1, Mirovich V.M.1
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Affiliations:
- Irkutsk State Medical University
- Issue: Vol 20, No 5-6 (2020)
- Pages: 170-174
- Section: Pharmacy
- URL: https://journal-vniispk.ru/2410-3764/article/view/42337
- DOI: https://doi.org/10.17816/2072-2354.2020.20.3.170-174
- ID: 42337
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Abstract
The article presents the method for the quantitative determination of flavonoids for the analysis of Bupleurum multinerve herb based on the method of differential spectrophotometry. The optimal conditions for analysis have been determined. They include extractant 40% ethyl alcohol, ratio of raw materials and extractant 1 : 100, extraction time of 60 minutes in the boiling water bath, complexing agent of 1 ml of 2% aluminum chloride. The use of rutin as a standard has been experimentally validated, analytical wavelength is 412 nm. The relative error of the mean result (for n = 9) was ±3.20%. Validation studies of the method have shown that it meets the criteria: linearity (r = 0.99988), correctness, specificity, and precision. The analytical range of the method is 8.67–26.08 μg/ml. The method is recommended for the inclusion into the new edition of the Pharmacopoeia Monograph for this type of plant material.
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##article.viewOnOriginalSite##About the authors
Svetlana A. Petukhova
Irkutsk State Medical University
Author for correspondence.
Email: lanapetukhova@gmail.com
Candidate of Pharmaceutical Sciences, Senior Lecturer, Department of Pharmacognosy and Pharmaceutical Technology
Russian Federation, IrkutskAlina A. Posokhina
Irkutsk State Medical University
Email: alinapos@yandex.ru
Applicant of the Department of Pharmacognosy and Pharmaceutical Technology
Russian Federation, IrkutskVera M. Mirovich
Irkutsk State Medical University
Email: mirko02@yandex.ru
Doctor of Pharmaceutical Sciences, Head of the Department of Pharmacognosy and Pharmaceutical Technology
Russian Federation, IrkutskReferences
- Асташенков А.Ю., Черемушкина В.А. Состояние ценопопуляций Bupleurum multinerve DC. в различных условиях Хакасии и Алтая // Растительный мир Азиатской России. – 2009. – Т. 1. – № 1. – С. 94–99. [Astashenkov A Ju, Cheremushkina VA. Sostojanie cenopopuljacij Bupleurum multinerve DC. v razlichnyh uslovijah Hakasii i Altaja. Rastitel’nyj mir Aziatskoj Rossii. 2009;1(1):94–99. (In Russ.)]
- Государственная фармакопея Российской Федерации. XIV издание [Электронный ресурс]. Режим доступа: http://www.femb.ru/femb/pharmacopea.php. Дата обращения: 10.09.2020. [Gosudarstvennaja farmakopeja Rossijskoj Federacii. XIV izdanie [Internet]. Available from: http://www.femb.ru/femb/pharmacopea.php. Accessed: 10.09.2020. (In Russ).]
- Минаева В.Г., Волхонская Т.А., Валуцкая А.Г. Сравнительное изучение флавоноидного состава некоторых сибирских видов володушки // Растительные ресурсы. – 1985. – Т. 21. – № 2. – С. 233–235. [Minaeva VG, Volhonskaja TA, Valuckaja AG. Sravnitel’noe izuchenie flavonoidnogo sostava nekotoryh sibirskih vidov volodushki. Rastitel’nye resursy. 1985;21(2):233–235. (In Russ.)]
- Саратиков А.С., Минаева В.Г., Лившиц Н.С. и др. Новые фармакологические свойства препарата буплерина из надземной части Bupleurum multinerve DC // Растительные ресурсы. – 1990. – Т. 26. – № 1. – С. 88–90. [Saratikov AS, Minaeva VG, Livshic NS, et al. Novye farmakologicheskie svojstva preparata buplerina iz nadzemnoj chasti Bupleurum multinerve DC. Rastitel’nye resursy. 1990;26(1):88–90. (In Russ.)]
- Altantsetseg S, Shatar S, Javzmaa N. Comparative study of essential oil constituents of Bupleurum species from Mongolia. Mongolian Journal of Chemistry. 2012;13(39):28–30. https://doi.org/10.5564/mjcv13i0.156.
- Olennikov DN, Partilkhaev VV. Flavonoids and phenylpropanoids from several species of Bupleurum growing in Buryatia. Chem Nat Compd. 2013;48(6):1078–1082. https://doi.org/10.1007/s10600-013-0471-x.
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