Email this article Assessment of the applicability of primarily identified natural luminescent bacteria, isolated from the azov and the black seas, to determine the antimicrobial activity of antibiotics
- Authors: Safronyuk S.L.1, Samolyuk V.V.1, Milova A.M.1, Havrichenko Y.Y.1, Katsev A.M.1
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Affiliations:
- Medical Academy named after S.I. Georgievsky of V.I. Vernadsky Crimean Federal University
- Issue: Vol 20, No 5-6 (2020)
- Pages: 175-183
- Section: Pharmacy
- URL: https://journal-vniispk.ru/2410-3764/article/view/84541
- DOI: https://doi.org/10.17816/2072-2354.2020.20.3.175-183
- ID: 84541
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Abstract
Five isolates of luminous bacteria from aquatic organisms of the Azov and the Black Seas were isolated. The study of morphological, cultural, physiological and biochemical properties showed that isolates M1 and M4 were the representatives of the species harveyi, and isolates Fb, Sh1, and B were the representatives of the species P. leiognathi. It was found that the strain P. leiognathi Sh1 was the most sensitive to zinc sulfate when studying its effect on allocated luminescent bacteria. The effective concentration that reduced the bioluminescent index (BLI) by 50% (EC50) for zinc sulfate, when exposed to the test strain, was 4,0 ± 0,1 μg/ml. Experimental data allowed to consider the strain P. leiognathi Sh1 to be the test-object for determining the antimicrobial activity of benzylpenicillin, gentamicin, streptomycin, tetracycline and ceftriaxone. The results of evaluating the effect of antibiotics on the test object, revealed that after 15 minutes of incubation, the BLI values decreased by 50% only in samples containing benzylpenicillin, gentamicin, and tetracycline. Their EC50 were 500.0, 283.0 and 28.5 μg/ml respectively. It was found that the exposure of test-strain to all antibacterial agents demonstrated resulted in decrease in BLI by 100% as compared to the control values. Strain P. leiognathi Sh1 can be used as a test-object for determining the antimicrobial activity of antibiotics.
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##article.viewOnOriginalSite##About the authors
Sergey L. Safronyuk
Medical Academy named after S.I. Georgievsky of V.I. Vernadsky Crimean Federal University
Author for correspondence.
Email: pharmalab01@mail.ru
Senior Lecturer, Department of Medical and Pharmaceutical Chemistry
Russian Federation, SimferopolVlada V. Samolyuk
Medical Academy named after S.I. Georgievsky of V.I. Vernadsky Crimean Federal University
Email: vlada.samolyuk.98@mail.ru
Postgraduate student, Department of Medical and Pharmaceutical Chemistry
Russian Federation, SimferopolAlena M. Milova
Medical Academy named after S.I. Georgievsky of V.I. Vernadsky Crimean Federal University
Email: MilovaAM21@gmail.com
3nd year student of the 2nd Medical Faculty
Russian Federation, SimferopolYuliia Yu. Havrichenko
Medical Academy named after S.I. Georgievsky of V.I. Vernadsky Crimean Federal University
Email: shkered.jolie@gmail.com
Assistant of the Department of Medical and Pharmaceutical Chemistry
Russian Federation, SimferopolAndrey M. Katsev
Medical Academy named after S.I. Georgievsky of V.I. Vernadsky Crimean Federal University
Email: katsev@mail.ru
Doctor of Biological Sciences, Professor, Head of the Department of Medical and Pharmaceutical Chemistry
Russian Federation, SimferopolReferences
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