Production of growth factors by fibroblasts in the conditions of the wound process and under the exposure of a bacterial biofilm matrix

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Abstract

Aim – to analyze the production of growth factors by fibroblasts in the wound process and under the influence of the bacterial biofilm matrix.

Material and methods. We assessed the levels of such growth factors as: VEGF, TGF-1β, GM-CSF, FGF produced by granulation tissue fibroblasts of acute wound (AW) (group 1, n=9) and chronic wound (CW) (group 2, n=17). Skin fibroblasts (n=5) were used for reference. The primary cultures were obtained by the method of explants. The experimental biofilm matrix included S. aureus, E. faecalis, A. baumannii, P. aeruginosa, K. pneumoniae, P. mirabilis (5 of each species).

Results. The elevated levels of TGF-1β and VEGF, secreted by the primary cultures of fibroblasts, against of the absence of changes in the values of FGF and GM-CSF, are the criteria for the imbalance of growth factors characteristic of CW. The impact of the biofilm matrix P. aeruginosa, K. pneumoniae, A. baumannii, P. mirabilis on skin fibroblasts was accompanied by an increase in the production of VEGF and GM-CSF and a decrease in the synthesis of TGF-1β and FGF. In the experiments with S. aureus and E. faecalis, a similar direction of changes was recorded, but the degree of its severity was lower than for experiments with the gram-negative bacteria.

Conclusion. The obtained results have demonstrated various effects of the biofilm matrix on the production of growth factors by fibroblasts and updated the information on the pathogenesis of CW formation. The revealed imbalance in the synthesis of growth factors by fibroblasts in the granulation tissue of wounds with “borderline” lifetimes (22–28 days) determines the need for tactical approaches in the treatment of CW.

About the authors

Yuliya I. Yarets

Republican Research Center for Radiation Medicine and Human Ecology

Author for correspondence.
Email: artyut@mail.ru
ORCID iD: 0000-0001-8879-5079
SPIN-code: 8038-5790

PhD, Associate Professor, Head of the Clinical Laboratory Medicine Department

Belarus, Gomel

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2. Figure 1. Device for filtering solutions containing microorganism cells.

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3. Figure 2. The level of growth factors produced by fibroblasts of the skin and granulation tissue of wounds.

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4. Figure 3. The level of production of growth factors by fibroblasts according to the results of the experiment with biofilm matrix components

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