Features of reparative myogenesis of the internal anal sphincter locally exposed to humic acids

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Abstract

Aim – to evaluate the effect of local exposure to humic substances on the repair of smooth muscle tissue of the internal anal sphincter.

Material and methods. Laboratory male rats were subjects for experimental study. The tissues were studied using light-electron microscopy and immunocytochemistry.

Results. The study revealed the features of myocyte survival in the wound and near-wound zones, the connective tissue scar formation rate. The phenomenon of the transformation of contractile myocytes into contractile-secretory ones was confirmed. The experiment has registered several features of the compensatory processes occurring with the use of humic acids as a local therapeutic agent. Specifically, the presence of a more pronounced edema of the interstitium, prolongation in time, in comparison with the control, filling the interstitium with connective tissue, as a result of which the architectonics of collagen fibers was adequate to the direction of myocyte contraction and relaxation. In addition, mitotically dividing cells were found among the muscle layer of the wound zone in the experimental group.

Conclusion. The experimental data suggests that humic acids, being a natural factor, can contribute to the processes of intracellular and cellular regeneration, resulting in better restoration of the internal sphincter structure. Further development of humic substances along with the research on their action at the cellular and tissue levels should be encouraged.

About the authors

Galina N. Suvorova

Samara State Medical University

Email: g.n.suvorova@samsmu.ru
ORCID iD: 0000-0002-0462-1344

PhD, the Head of the Department of Histologya and Embriology

Russian Federation, 227 Chapaevskaya st., Samara, 443001

Antonina S. Pronina

Samara State Medical University

Author for correspondence.
Email: a.s.pronina@samsmu.ru
ORCID iD: 0000-0003-4143-5628

senior lecturer of the Department of Histology and Embryology

Russian Federation, Samara

Olesya V. Kulakova

Samara State Medical University

Email: o.v.kulakova@samsmu.ru
ORCID iD: 0000-0002-8318-0355

PhD, Associate professor, Department of Histology and Embryology

Russian Federation, Samara

Mariya A. Krivopalova

Samara State Medical University

Email: m.a.krivopalova@samsmu.ru
ORCID iD: 0000-0002-9461-5326

PhD, Associate professor of the Department of Medical Chemistry

Russian Federation, 89 Chapaevskaya st., Samara, 443099

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Supplementary files

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2. Figure 1. Smooth muscle tissue of the internal anal sphincter. 24 hours after overstretching. Contact between myocytes is broken, interstitium edema begins. Staining with hematoxylin and eosin. Ob. 100, oc. 15.

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3. Figure 2. Ultrastructure of myocytes of the internal anal sphincter. 3rd day after overstretching.

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4. Figure 3. Wound zone of the internal anal sphincter. 7th day after overstretching. Staining with hematoxylin and eosin. Ob. 100, oc. 15.

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5. Figure 4. Ultrastructure of myocytes of the internal anal sphincter. 7th day after overstretching. The arrows indicate the sites of collagen filament synthesis.

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6. Figure 5. Internal anal sphincter. Experimental group, 15th day of the experiment. In the interstitium between the compartments of the remaining myocytes, the arrow indicates the tissue basophil. Staining with hematoxylin and eosin. Ob. 100, oc. 15.

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7. Figure 6. Internal anal sphincter. Mitotically dividing myocyte in the wound zone. Immunohistochemical reaction with Ki-67. Finishing – hematoxylin. Ob. 100, oc. 15.

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Copyright (c) 2023 Suvorova G.N., Pronina A.S., Kulakova O.V., Krivopalova M.A.

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