Email this article Effect of DNA methylation on the interaction with tetrahydrocortisol–apolipoprotein A-I complex
- Authors: Kuznetsov P.A.1, Zaitsev B.N.2, Gimautdinova O.I.1,3, Demchenko E.N.4, Panin L.E.1
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Affiliations:
- Scientific Research Institute of Biochemistry
- State Research Center for Virology and Biotechnology “Vektor”, pos.
- Novosibirsk State Medical University
- Scientific Research Institute of Fundamental and Clinical Immunology
- Issue: Vol 11, No 5-6 (2016)
- Pages: 364-370
- Section: Article
- URL: https://journal-vniispk.ru/2635-1676/article/view/219403
- DOI: https://doi.org/10.1134/S1995078016030101
- ID: 219403
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Abstract
The effect of GCNGC site methylation on the DNA binding to tetrahydrocortisol–apolipoprotein A-I complex was shown by atomic force microscopy and enzymatic analysis. Methylation of GCNGC sites alters the mechanism of interaction of tetrahydrocortisol–apolipoprotein A-I complex with DNA and leads to the aggregation of the DNA-protein complexes. It was assumed that the involvement of complexes of steroid hormones with apolipoprotein A-I in the process of chromatin restructuring is associated with the processes of DNA methylation/demethylation.
About the authors
P. A. Kuznetsov
Scientific Research Institute of Biochemistry
Author for correspondence.
Email: pawelkuzn@mail.ru
Russian Federation, ul. Timakova 2, Novosibirsk, 630060
B. N. Zaitsev
State Research Center for Virology and Biotechnology “Vektor”, pos.
Email: pawelkuzn@mail.ru
Russian Federation, Kol’tsovo, Novosibirsk oblast, 630559
O. I. Gimautdinova
Scientific Research Institute of Biochemistry; Novosibirsk State Medical University
Email: pawelkuzn@mail.ru
Russian Federation, ul. Timakova 2, Novosibirsk, 630060; Krasnyi pr. 42a, Novosibirsk, 630099
E. N. Demchenko
Scientific Research Institute of Fundamental and Clinical Immunology
Email: pawelkuzn@mail.ru
Russian Federation, ul. Yadrintsevskaya 14, Novosibirsk, 630099
L. E. Panin
Scientific Research Institute of Biochemistry
Email: pawelkuzn@mail.ru
Russian Federation, ul. Timakova 2, Novosibirsk, 630060
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