Control of Adsorption Horseradish Peroxidase on the Surface of Glass Multicapillary by Using a Polyelectrolyte on Layer-by-Layer Technology

Abstract

This work aims to explore the effect of polyelectrolyte layers on the protein adsorption using the example horseradish peroxidase (HRP) in a glass multicapillary to develop ways to control the selective sorption. It is shown that a more effective adsorption of HRP was observed for the bilayer polyethylene (PEI)/polystyrene sulfonate (PSS). This effect is explained by the fact that the polycation PEI modifies the glass surface, and the polyanion PSS creates a layer for the HRP effective sorption.