The latent period changes in the pain response of rats with prolonged consumption of food concentrates “Resveratrol” and “Enoant”

The biological effectiveness of polyphenols is significant, including multiple effects, such as antioxidant, immunomodulatory, cardioprotective, neuroprotective and anti-inflammatory, among others [5; 6]. Recent scientific studies have demonstrated the analgesic potential of natural polyphenols, which was directly shown in pain tests on animals [9]. The goal of this study was to compare the analgesic activity of a dietary supplement containing high concentrations of grape polyphenols, such as the food concentrate "Enoant" and the resveratrol with aspirin.

Methods. The study was carried out in two stages. In the first stage, 40 male Wistar rats aged between 7,5 and 8 months were divided into four groups: control (10 rats), aspirin (10 rats), the "resveratrol" (10 rats), and the food concentrate "Enoant" (10 rats). The rats in the resveratrol group were given a solution of resveratrol orally at a dose of 20 mg/kg per day for a period of 30 days. The rats in the food concentrate "Enoant" group were given a corresponding solution orally at the same dose per day for the same period. All groups were tested in the Hot Plate Test on 15 and 30 days. Aspirin (“Merk”, Germany) in the form of a suspension was administered orally only on testing days (0.4 ml) using a standard probe at a dose of 100 mg/kg 90 minutes before recording the latent period of pain response (LPPR) in the “Hot Plate” test.

40 male Wistar rats aged between 7,5 and 8 months were used in Tail Flick Test. The approach and all the methods techniques were corresponded to the Hot Plate Test.

At the second stage of the study, we repeated all the described research activities, but with an important difference: the testing was performed only on the 30th day with oral administration of aspirin at a dose of 100 mg/kg in the groups of the “Aspirin”, “Resveratrol + Aspirin” and “Enoant + Aspirin” groups against the background of thirty-days intake of Resveratrol and Enoant.

Statistical data processing was performed in GraphPad Prism 8, using the Shapiro-Wilk test, the Kruskal-Wallis test and the Dunn's test. The paired comparison used the Mann-Whitney U test. Differences were considered at significant p<0,05.

Results. It was shown that a thirty-day consumption of resveratrol and the food concentrate "Enoant" at a dose of 20 mg/kg resulted in a significant analgesic effect, similar to the analgesic effect of aspirin. Therefore, a thirty-day consumption of the Crimean alcohol-free the food concentrate "Enoant" has a pronounced analgesic action that is similar to that of resveratrol and aspirin. It means that the food concentrate "Enoant" with is rich of polyphenols, vitamins, and bioelements, but also a promising pain reliever that could help alleviate the symptoms of chronic pain and improve the quality of life for people suffering with similar health problems.