Post-mortem diagnosis of pig pasterellosis
- Autores: Akobian A.R.1
-
Afiliações:
- Armenian National Agrarian University
- Edição: Volume 17, Nº 2 (2025)
- Páginas: 610-627
- Seção: Experience of Regions
- ##submission.datePublished##: 30.04.2025
- URL: https://journal-vniispk.ru/2658-6649/article/view/311065
- DOI: https://doi.org/10.12731/2658-6649-2025-17-2-1086
- EDN: https://elibrary.ru/FBSWNB
- ID: 311065
Citar
Texto integral
Resumo
Background. Due to the widespread spread of pasteurellosis among animals, particularly in pigs and the enormous economic damage it causes to pig farms due(significant loss of pig population, treatment costs, decreased quality of products and the danger of infection of people), the problem of diagnosing pasteurellosis is an urgent task of veterinary science.
Purpose. The purpose of our research was post-mortem diagnosis of pasteurelosis in three pig farms in the Armavir region of the Republic of Armenia, identification of its causative agent, with the aim of properly organizing treatment and preventive measures
Material and methods. The study was carried out in three pig farms in the Armavir region. A post-mortem examination of the lungs of pigs was carried out to study the nature of their lesions, and the average weight of slaughtered animals was determined. Pathological material was selected from the affected parts of the lungs, which was used for culturing and identifying the pathogen. Identification of the pathogen was carried out on blood agar, Mac Conkey agar. Determination of the biochemical properties of the pathogen was carried out using biochemical tests API 20E.
Results. In 74 slaughtered pigs, pathological changes in the lungs were characteristic of lobar, serous-fibrinous pneumonia, fibrino-necrotic pleuropneumonia, pleurisy and bronchopneumonia, and a decrease in body weight was observed. When inoculated on MPA containing 5℅ serum, colonies with morphological properties characteristic of pasteurel colonies were found. Biochemical studies confirmed that the pathogen belongs to the species P. multocida.
Conclusion. During examination the lungs of slaughtered animals, pneumonia, pleuropnemonia and pleurisy were found in the majority of the lungs. When inoculated on nutrient media, the pathogen P. Multocida was identified. Summarizing the research results, we can conclude that pasteurelosis is widespread, accompanied by lung damage.
Palavras-chave
Sobre autores
Anush Akobian
Armenian National Agrarian University
Autor responsável pela correspondência
Email: akobian.anush@yandex.ru
ORCID ID: 0009-0002-7781-3045
Researcher, Research Center for Veterinary Medicine and Veterinary and Sanitary Expertise
Armênia, 74, Teryan Str., Yerevan, 0009, Republic of Armenia
Bibliografia
- Kimura, R., Hayashi, Y., Takeuchi, T., Shimizu, M., Iwata, M., Tanahashi, J., & Ito, M. (2004). Pasteurella multocida septicemia caused by close contact with a domestic cat: case report and literature review. J Infect Chemother, 10(4), 250-252. https://doi.org/10.1007/s10156-004-0331-5
- Лях, Ю. Г. (2013). Пастереллез свиней и крупного рогатого скота. Минск: ООО «Инфофорум», 212 с. (Lyakh, Yu. G. (2013). Pasteurellosis of pigs and cattle. Minsk: Inforum. 212 p.)
- Лях, Ю. Г., Синица, О. Н. (2000). Пневмонии пастереллезно-сальмонеллезной этиологии молодняка сельскохозяйственных животных в Республике Беларусь и их специфическая профилактика. Международный аграрный журнал, 9, 42-44. (Lyakh, Yu. G., & Sinitsa, O. N. (2000). Pneumonia of pasteurella-salmonella etiology in young farm animals in the Republic of Belarus and their specific prevention. International Agricultural Journal, 9, 42-44.) EDN: https://elibrary.ru/ytupcs
- Немкова, Н. П. (2017). Ветеринарно-санитарная экспертиза продуктов убоя животных при инфекционных болезнях: Методические указания. Красноярск, 73 с. (Nemkova, N. P. (2017). Veterinary and sanitary examination of slaughter products of animals with infectious diseases: Methodical instructions. Krasnoyarsk. 73 p.) EDN: https://elibrary.ru/gdgzpy
- Bardou, M., Honnorat, E., Dubourg, G., Couderc, C., Fournier, P. E., Seng, P., & Stein, A. (2015). Meningitis caused by Pasteurella multocida in a dog owner without a dog bite: clonal lineage identification by MALDI-TOF mass spectrometry. BMC Res Notes, 8, 626. https://doi.org/10.1186/s13104-015-1615-9
- Becskei, Z., Aleksić-Kovačević, S., Rusvai, M., Balka, G., Jakab, C., Petrović, T., & Knežević, M. (2010). Distribution of porcine circovirus 2 cap antigen in the lymphoid tissue of pigs affected by postweaning multisystemic wasting syndrome. Acta Vet Hung, 58(4), 483-498. https://doi.org/10.1556/AVet.58.2010.4.9
- Blackall, P. J., Pahoff, J. L., & Bowles, R. (1997). Phenotypic characterisation of Pasteurella multocida isolates from Australian pigs. Vet Microbiol, 57(4), 355-360. https://doi.org/10.1016/s0378-1135(97)00111-9
- Boyanton, B. L. Jr., Freij, B. J., Robinson-Dunn, B., Makin, J., Runge, J. K., & Luna, R. A. (2016). Neonatal Pasteurella multocida subsp. septica Meningitis Traced to Household Cats: Molecular Linkage Analysis Using Repetitive-Sequence-Based PCR. J Clin Microbiol, 54(1), 230-232. https://doi.org/10.1128/JCM.01337-15
- Carter, G. R. (1955). Studies on Pasteurella multocida. I. A hemagglutination test for the identification of serological types. Am J Vet Res, 16(60), 481-484.
- Christidou, A., Maraki, S., Gitti, Z., & Tselentis, Y. (2005). Review of Pasteurella Multocida Infections over a Twelve-Year Period in a Tertiary Care Hospital. American Journal of Infectious Diseases, 1(2), 107-110. https://doi.org/10.3844/ajidsp.2005.107.110
- Čobanović, N., Karabasil, N., Ilić, N., Dimitrijević, M., Vasilev, D., Cojkić, A., & Jankvić, L. (2015). Pig welfare assessment based on presence of skin lesions on carcass and pathological findings in organs. Proceedings of the 17th International Congress on Animal Hygiene, Animal Hygiene and Welfare in Livestock Production. The First Step to Food Hygiene, 26-29.
- Culling, C. F. A. (1974). Handbook of Histopathological and Histochemical Techniques. Including Museum Techniques. Butterworths, 687 p.
- Dailidavičienė, J., Januškevičienė, G., June, V., Pockevičius, A., & Kerzienė, S. (2008). Typically definable respiratory lesions and their influence on meat characteristics in pigs. Veterinarija ir Zootechnika, 43(65), 20-24.
- Dailidavičienė, J., Januškevičienė, G., Zaborskiene, G., & Gear miele, G. (2009). Pork quality analysis according to different degree of lung lesions. Fleischwirtschaft, 89(1), 100-103.
- Davies, R. L., MacCorquodale, R., Baillie, S., & Caffrey, B. (2003). Characterization and comparison of Pasteurella multocida strains associated with porcine pneumonia and atrophic rhinitis. J Med Microbiol, 52(Pt 1), 59-67. https://doi.org/10.1099/jmm.0.05019-0
- Davies, R. L., MacCorquodale, R., & Reilly, S. (2004). Characterisation of bovine strains of Pasteurella multocida and comparison with isolates of avian, ovine and porcine origin. Vet Microbiol, 99(2), 145-158. https://doi.org/10.1016/j.vetmic.2003.11.013
- Fegan, N., Blackall, P. J., & Pahoff, J. L. (1995). Phenotypic characterisation of Pasteurella multocida isolates from Australian poultry. Vet Microbiol, 47(3-4), 281-286. https://doi.org/10.1016/0378-1135(95)00119-0
- Fernández-Valencia, J. A., García, S., & Prat, S. (2008). Pasteurella multocida septic shock after a cat scratch in an elderly otherwise healthy woman: a case report. Am J Emerg Med, 26(3), 380.e1-3. https://doi.org/10.1016/j.ajem.2007.05.019
- Fraile, L., Alegre, A., López-Jiménez, R., Nofrarías, M., & Segalés, J. (2010). Risk factors associated with pleuritis and cranio-ventral pulmonary consolidation in slaughter-aged pigs. Vet J, 184(3), 326-333. https://doi.org/10.1016/j.tvjl.2009.03.029
- Guillet, C., Join-Lambert, O., Carbonnelle, E., Ferroni, A., & Vachée, A. (2007). Pasteurella multocida sepsis and meningitis in 2-month-old twin infants after household exposure to a slaughtered sheep. Clin Infect Dis, 45(6), e80-e81. https://doi.org/10.1086/520979
- Heddleston, K. L., Gallagher, J. E., & Rebers, P. A. (1972). Fowl cholera: gel diffusion precipitin test for serotyping Pasteurella multocida from avian species. Avian Dis, 16(4), 925-936.
- Hillen, S., von Berg, S., Köhler, K., Reinacher, M., Willems, H., & Reiner, G. (2014). Occurrence and severity of lung lesions in slaughter pigs vaccinated against Mycoplasma hyopneumoniae with different strategies. Prev Vet Med, 113(4), 580-588. https://doi.org/10.1016/j.prevetmed.2013.12.012
- Holst, E., Rollof, J., Larsson, L., & Nielsen, J. P. (1992). Characterization and distribution of Pasteurella species recovered from infected humans. J Clin Microbiol, 30(11), 2984-2987. https://doi.org/10.1128/jcm.30.11.2984-2987.1992
- Hotchkiss, E. J., Hodgson, J. C., Schmitt-van de Leemput, E., Dagleish, M. P., & Zadoks, R. N. (2011). Molecular epidemiology of Pasteurella multocida in dairy and beef calves. Vet Microbiol, 151(3-4), 329-335. https://doi.org/10.1016/j.vetmic.2011.03.018
- Karesh, W. B., Cook, R. A., Bennett, E. L., & Newcomb, J. (2005). Wildlife trade and global disease emergence. Emerg Infect Dis, 11(7), 1000-1002. https://doi.org/10.3201/eid1107.050194
- Karesh, W. B., & Noble, E. (2009). The bushmeat trade: increased opportunities for transmission of zoonotic disease. Mt Sinai J Med, 76(5), 429-434. https://doi.org/10.1002/msj.20139
- Kofteridis, D. P., Christofaki, M., Mantadakis, E., Maraki, S., Drygiannakis, I., Papadakis, J. A., & Samonis, G. (2009). Bacteremic community-acquired pneumonia due to Pasteurella multocida. Int J Infect Dis, 13(3), e81-e83. https://doi.org/10.1016/j.ijid.2008.06.023
- Liu, H., Zhao, Z., Xi, X., Xue, Q., Long, T., & Xue, Y. (2017). Occurrence of Pasteurella multocida among pigs with respiratory disease in China between 2011 and 2015. Ir Vet J, 70, 2. https://doi.org/10.1186/s13620-016-0080-7 EDN: https://elibrary.ru/nhtxsu
- López, C., Sanchez-Rubio, P., Betrán, A., & Terré, R. (2013). Pasteurella multocida bacterial meningitis caused by contact with pigs. Braz J Microbiol, 44(2), 473-474. https://doi.org/10.1590/S1517-83822013000200021
- Mac Fadin, J. F. (2000). Biochemical Tests for Identification of Medical Bacteria. 3rd Ed., Lippincott Williams & Wilkins, Pennsylvania, USA, 912 p.
- Minkus, D., Schutte, A., von Mickwitz, G., & Beutling, D. (2004). Lung health, meat content and meat ripening in pigs - defective lungs as a problem in meat inspection. Fleischwirtschaft-Frankfurt, 87(7), 110-113.
- Oehler, R. L., Velez, A. P., Mizrachi, M., Lamarche, J., & Gomp, S. (2009). Bite-related and septic syndromes caused by cats and dogs. The Lancet Infectious Diseases, 9(7), 439-447. https://doi.org/10.1016/S1473-3099(09)70110-0 EDN: https://elibrary.ru/mmfypv
- Ostanello, F., Dottori, M., Gusmara, C., Leotti, G., & Sala, V. (2007). Pneumonia disease assessment using a slaughterhouse lung-scoring method. J Vet Med A Physiol Pathol Clin Med, 54(2), 70-75. https://doi.org/10.1111/j.1439-0442.2007.00920.x
- Ozbey, G., Kilic, A., Ertas, H. B., & Muz, A. (2004). Random amplified polymorphic DNA (RAPD) analysis of Pasteurella multocida and Mannheimia haemolytica strains isolated from cattle, sheep and goats. Vet Med Czech, 49(3), 65-69. https://vetmed.agriculturejournals.cz/pdfs/vet/2004/03/01.pdf
- Permentier, L., Maenhout, D., Deley, W., Broekman, K., Vermeulen, L., Agten, S., Verbeke, G., Aviron, J., & Geers, R. (2015). Lung lesions increase the risk of reduced meat quality of slaughter pigs. Meat Sci, 108, 106-108. https://doi.org/10.1016/j.meatsci.2015.06.005
- Rajkhowa, S., Shakuntala, I., Pegu, S., Das, R., & Das, A. (2012). Detection of Pasteurella multocida isolates from local pigs of India by polymerase chain reaction and their antibiogram. Trop Anim Health Prod, 44(7), 1497-1503. https://doi.org/10.1007/s11250-012-0094-4 EDN: https://elibrary.ru/uyllxs
- Reaser, J. K., Clark, E. E. Jr., & Meyers, N. M. (2008). All creatures great and minute: a public policy primer for companion animal zoonoses. Zoonoses Public Health, 55(8-10), 385-401. https://doi.org/10.1111/j.1863-2378.2008.01123.x
- Rimler, R. B., & Rhoades, K. R. (1987). Serogroup F, a new capsule serogroup of Pasteurella multocida. J Clin Microbiol, 25(4), 615-618. https://doi.org/10.1128/jcm.25.4.615-618.1987
- Smith, K. M., Anthony, S. J., Switzer, W. M., Epstein, J. H., Seimon, T., Jia, H., Sanchez, M. D., Huynh, T. T., Galland, G. G., Shapiro, S. E., Sleeman, J. M., McAloose, D., Stuchin, M., Amato, G., Kolokotronis, S. O., Lipkin, W. I., Karesh, W. B., Daszak, P., & Marano, N. (2012). Zoonotic viruses associated with illegally imported wildlife products. PLoS One, 7(1), e29505. https://doi.org/10.1371/journal.pone.0029505 EDN: https://elibrary.ru/ycmovl
- Štukelj, M., Plut, J., & Toplak, I. (2015). Serum inoculation as a possibility for elimination of porcine reproductive and respiratory syndrome (PRRS) from a farrow-to-finish pig farm. Acta Vet Hung, 63(3), 389-399. https://doi.org/10.1556/004.2015.037
- Szeredi, L., Cságola, A., Dán, Á., & Dencső, L. (2015). Vascular lesions and pneumonia in a pig fetus infected by porcine circovirus type 2. Acta Vet Hung, 63(2), 215-222. https://doi.org/10.1556/004.2015.019
- Toplak, I., Lazić, S., Lupulović, D., Prodanov-Radulović, J., Becskei, Z., Došen, R., & Petrović, T. (2012). Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia. Acta Vet Hung, 60(3), 409-420. https://doi.org/10.1556/AVet.2012.035
- Weber, D. J., Wolfson, J. S., Swartz, M. N., & Hooper, D. C. (1984). Pasteurella multocida infections. Report of 34 cases and review of the literature. Medicine (Baltimore), 63(3), 133-154.
- Weese, J. S., McCarthy, L., Mossop, M., Martin, H., & Lefebvre, S. (2007). Observation of practices at petting zoos and the potential impact on zoonotic disease transmission. Clin Infect Dis, 45(1), 10-15. https://doi.org/10.1086/518572
- Wilson, B. A. (2008). Global biosecurity in a complex, dynamic world. Complexity, 14(1), 71-88. https://doi.org/10.1002/cplx.20246
- Wilson, B. A., & Ho, M. (2013). Pasteurella multocida: from zoonosis to cellular microbiology. Clin Microbiol Rev, 26(3), 631-655. https://doi.org/10.1128/CMR.00024-13 EDN: https://elibrary.ru/yaeeea
- Woolhouse, M. E., & Gowtage-Sequeria, S. (2005). Host range and emerging and reemerging pathogens. Emerg Infect Dis, 11(12), 1842-1847. https://doi.org/10.3201/eid1112.050997
- Woodford, M. H. (2009). Veterinary aspects of ecological monitoring: the natural history of emerging infectious diseases of humans, domestic animals and wildlife. Trop Anim Health Prod, 41(7), 1023-1033. https://doi.org/10.1007/s11250-008-9269-4
Arquivos suplementares
