Modular nanotransporters containing Keap1 monobodies are capable of reducing the toxic effect of acetaminophen on the liver of mice

Yu. V. Khramtsov; Храмцов Ю. В.; A. A. Rosenkranz; Розенкранц А. А.; A. V. Ulasov; Уласов А. В.; T. A. Slastnikova; Сластникова Т. А.; T. N. Lupanova; Лупанова Т. Н.; R. T. Alieva; Алиева Р. Т.; G. P. Georgiev; Георгиев Г. П.; A. S. Sobolev; Соболев А. С.

doi:10.31857/S2686738925020109

Modular nanotransporters containing Keap1 monobodies are capable of reducing the toxic effect of acetaminophen on the liver of mice

作者: Khramtsov Y.V.¹, Rosenkranz A.A.¹^,2, Ulasov A.V.¹, Slastnikova T.A.¹, Lupanova T.N.¹, Alieva R.T.¹, Georgiev G.P.¹, Sobolev A.S.¹^,2
隶属关系:
1. Institute of Gene Biology, RAS
2. Lomonosov Moscow State University
期: 卷 521, 编号 1 (2025)
页面: 225-228
栏目: Articles
URL: https://journal-vniispk.ru/2686-7389/article/view/306218
DOI: https://doi.org/10.31857/S2686738925020109
ID: 306218

如何引用文章

全文:

开放存取

##reader.subscriptionAccessGranted##
受限制的访问

订阅存取

详细
作者简介
参考
补充文件
统计

详细

Previously, we created a modular nanotransporter (MNT) containing a monobody to Keap1, an intracellular protein inhibitor of the Nrf2 transcription factor that controls cellular protection from oxidative stress and is capable of interacting with Keap1 in hepatocytes and protect this cells from the effects of hydrogen peroxide. Oxidative liver damage by acetaminophen was used as a model to study the antitoxic effect of this MNT. Intraperitoneal injection of acetaminophen to mice resulted in an increase in the level of alanine aminotransferase and aspartate aminotransferase in the blood, as well as in liver edema. A significant decrease in the level of these enzymes in the blood, along with a decrease in liver edema, was observed after preliminary intravenous administration of MNT 2 hours before the acetaminophen injection. The results obtained can serve as a basis for creating drugs aimed at treating diseases associated with oxidative stress.

关键词

Nrf2, Keap1, modular nanotransporters, monobodies, Nrf2, Keap1, acetaminophen, ALT, AST, single-photon emission computed tomography

参考

Bellezza I., Giambanco I., Minelli A., et al. // Acta Mol. Cell Res. 2018. V. 1865(5). P. 721–733.
Hayes J.D., Dinkova-Kostova A.T. // Trends Biochem. Sci. 2014. V. 39(4). P. 199–218.
Ulasov A.V., Rosenkranz A.A., Georgiev G.P., et al. // Life Sci. 2022. V. 291. 120111.
Robledinos-Anton N., Fernandez-Gines R., Manda G., et al. // Oxid. Med. Cell Longev. 2019. V. 2019. 9372182.
Ngo V., Duennwald M.L. // Antioxidants. (Basel). 2022. V. 11(12).
Taguchi K., Kensler T.W. // Arch. Pharm. Res. 2020. V. 43(3). P. 337–349.
Patra U., Mukhopadhyay U., Sarkar R., et al. // Antivir. Res. 2019. V. 161. P. 53–62.
Olagnier D., Farahani E., Thyrsted J., et al. // Nat. Commun. 2020. V. 11. 4938.
Khramtsov Y.V., Ulasov A.V., Slastnikova T.A., et al. // Pharmaceutics. 2023. V. 15. 2687.
Khramtsov Y.V., Ulasov A.V., Rosenkranz A.A., et al. // Pharmaceutics. 2024. V. 16. 1345.
Lee W.M. // Hepatol. 2017. V. 67. P. 1324–1331.
McGill M.R., Williams C.D., Xie Y., et al. // Toxicol. Appl. Pharmacol. 2012. V. 264. P. 387–394.
Vorobyeva A., Bragina O., Altai M., et al. // Contrast. Media Mol. Imaging. 2018. V. 2018. 6930425.
Steffens M. G., Kranenborg M.H., O.C. Boerman O.C., et al. // Cancer Biother. Radiopharm. 1998. V. 13. P. 133–139.
Ferris T., Carroll L., Jenner S., et al. // J. Labelled Comp Radiopharm. 2021. V. 64. P. 92–108.
Bruinstroop E., van der Spek A.H., Boelen A. // Eur. Thyroid J. 2023. V. 12. e220211.
Dohan O., De la Vieja A., Paroder V., et al. // Endocr. Rev. 2003. V. 24. P. 48–77.
Shen Z., Wang Y., Su Z., et al. // Chem. Biol. Interact. 2018. V. 282. P. 22–28.

补充文件

附件文件

动作

1. JATS XML

下载

用户名
密码
记住我

忘记您的密码?	注册

用户名
密码
记住我

忘记您的密码?	注册

卷 520, 编号 1 (2025)

Modular nanotransporters containing Keap1 monobodies are capable of reducing the toxic effect of acetaminophen on the liver of mice

全文:

详细

关键词

作者简介

Yu. Khramtsov

A. Rosenkranz

A. Ulasov

T. Slastnikova

T. Lupanova

R. Alieva

G. Georgiev

A. Sobolev

参考

补充文件