Email this article MAST CELLS AFTER BONE MARROW AUTOTRANSPLANTATION
- Authors: Vorobyova OV1, Lyubovtseva LA1, Guryanova EA1, Gimaldinova NE1
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Affiliations:
- The Department of General and Clinical Morphology and Forensic Medicine Chuvash State University I.N. Ulyanova
- Issue: Vol 19, No 1S (2019)
- Pages: 45-47
- Section: Articles
- URL: https://journal-vniispk.ru/MAJ/article/view/19318
- ID: 19318
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Abstract
Purpose: to study bone marrow mast cells in mice after autotransplantation. Materials and methods. Mast cells and sulphatedness degree of heparin mucopolysaccharide in the bone marrow were identified using Unna staining with polychromatic toluidine blue. Study results. Data on mast cells distribution in the bone marrow after autotransplantation in 40 minutes and in 2 hours were analyzed. Two types of mast cells’ reaction to autologous bone marrow introduction were identified: sequential degranulation and exocytosis. As a result, secretion of mast cells causes production of biologically active substances in the microenvironment of a mast cell; these substances form chains and conglomerates from granules, changes in the number of which can contribute to changing the process of mitotic division. According to morphological criteria three populations of mast cells were identified: small-sized cells, with blue-colored nuclei stained orthochromatically and located asymmetrically; metachromatic oval-shaped cells with a nucleus located in the centre; large, degranulated, gamma-metachromatic mast cells. Most part of mast cells in bone marrow smears is presented by integral forms. Heparin in mast cells takes part in regulating metabolism in the bone marrow after autotransplantation.
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##article.viewOnOriginalSite##About the authors
O V Vorobyova
The Department of General and Clinical Morphology and Forensic Medicine Chuvash State University I.N. Ulyanova
L A Lyubovtseva
The Department of General and Clinical Morphology and Forensic Medicine Chuvash State University I.N. Ulyanova
E A Guryanova
The Department of General and Clinical Morphology and Forensic Medicine Chuvash State University I.N. Ulyanova
N E Gimaldinova
The Department of General and Clinical Morphology and Forensic Medicine Chuvash State University I.N. Ulyanova
References
- Kutukova NA, Nazarov PG, Kudryavtseva GV, Shishkin VI. Mast cells and aging. Advances in geronto¬logy. 2016;29(4):586-593.
- Forsythe P. Mast Cells in Neuroimmune Interactions. Trends Neurosci. 2019;42(1):43-55. https://doi.org/10.1016/j.tins.2018.09.006.
- Gimaldinova NE, Ignatieva EN, Lyubovtseva LA, et al. The effects of cycloferon on the distribution of neuroamines in bioamine-containing structures of the spleen. Journal of New Medical Technologies. 2018;25(3):101-106.
- Guryanova EA, Lyubovtseva LA, Shabukova AA. The study of the functional state of mast cells of the skin in the field of acupuncture points after acupuncture. Nizhny Novgorod Medical Journal. 2008;(5):110.
- Vorobeva OV. Dynamics of morpho-functional conditions of cellular programmed differentiation in bone marrow as a hemopoietic organ. Zhurnal anatomii i gistopatologii. 2017;6(2):26-29.
- Vorobeva OV. Neurally mediated biogenic amines in bone marrow structures in allografting. Morfologicheskie vedomosti. 2016;24(4):89-91.
- Chesnokova NP. Haematopoiesis and its regulation at various stages of differentiation of hematopoietic bone marrow cells. Saratov Journal of medical scientific research. 2012;8(3):711-719.
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