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Post a Comment Morphological characteristic of inguinal lymph nodes in HIV infection
- Authors: Gulyaeva N.I.1, Freind G.G.1, Shmagel N.G.2, Korolevskaya L.B.3, Saidakova E.V.3, Shmagel K.V.3
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Affiliations:
- E.A. Vagner Perm State Medical University
- Perm Regional Center for Prevention and Fight against AIDS and Infectious Diseases
- Institute of Ecology and Genetics of Microorganisms of the Ural Branch of RAS
- Issue: Vol 36, No 2 (2019)
- Pages: 51-59
- Section: Methods of diagnosis and technologies
- URL: https://journal-vniispk.ru/PMJ/article/view/14324
- DOI: https://doi.org/10.17816/pmj36251-59
- ID: 14324
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Abstract
Aim. To assess the morphological changes in inguinal lymph nodes among patients with stage 4a HIV infection, who receive antiretroviral therapy.
Materials and methods. There were examined 12 HIV-infected patients with stage 4a, treated at “Perm Regional Center for Prevention and Fight against AIDS and Infectious Diseases”, who received antiretroviral therapy for 2 years. Two groups of patients were formed: group I – 6 persons, whose CD+ blood lymphocyte number was more than 350 in 1 mcl; group II – 6 persons with CD+ blood lymphocyte number less than 350 in 1 mcl. Inguinal lymph nodes (ILN) were taken under local anesthesia, histological preparations were prepared by traditional scheme, stained with hematoxylin and eosin using Masson three-colour staining. In immunohistochemical reactions expression of Ki-67 and CD+ markers was estimated.
Results.Histoarchitectonics of lymph nodes was changed as a result of massive development of sclerosis in the region of hilum, capsule and trabecules. Against the background of sclerosis, there occurred lymphocyte depletion, change in structure of stromal cells and neoangiogenesis in all the zones of the lymph node. In the regions of sclerosis, death of lymphocytes was revealed. In patients of the second group, more active development of follicles with the centers of reproduction in the lymph node cortical substance, as well as growth of the number of Ki-67 maker-expressing cells was established
Conclusions.In the inguinal lymph nodes, the development of sclerotic processes causes the death of T-lymphocytes, which, in their turn, are the source of lymphotoxin-β formation. Loss of CD+-T-lymphocytes is accompanied by deficit of lymphotoxin-β and induces the loss of fibroblastic reticular cells themselves, which through the production of IL-7 support the vital activity of T-cells.
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##article.viewOnOriginalSite##About the authors
N. I. Gulyaeva
E.A. Vagner Perm State Medical University
Author for correspondence.
Email: bizon55@mail.ru
к.м.н., доцент кафедры гистологии, цитологии и эмбриологии
Russian Federation, 26, Petropavlovskay street, Perm, 614000G. G. Freind
E.A. Vagner Perm State Medical University
Email: bizon55@mail.ru
д.м.н., профессор, заведующая кафедрой патологической анатомии с секционным курсом
Russian Federation, 26, Petropavlovskay street, Perm, 614000N. G. Shmagel
Perm Regional Center for Prevention and Fight against AIDS and Infectious Diseases
Email: bizon55@mail.ru
к.м.н., врач-иммунолог
Russian Federation, 13, Goleva str., Perm, 614081L. B. Korolevskaya
Institute of Ecology and Genetics of Microorganisms of the Ural Branch of RAS
Email: bizon55@mail.ru
к.м.н., научный сотрудник
Russian Federation, 21, Sviyazeva str., Perm, 614088E. V. Saidakova
Institute of Ecology and Genetics of Microorganisms of the Ural Branch of RAS
Email: bizon55@mail.ru
к.б.н., младший научный сотрудник
Russian Federation, 21, Sviyazeva str., Perm, 614088K. V. Shmagel
Institute of Ecology and Genetics of Microorganisms of the Ural Branch of RAS
Email: bizon55@mail.ru
д.м.н., заведующий лабораторией
Russian Federation, 21, Sviyazeva str., Perm, 614088References
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