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Post a Comment Orexin A content in brain structures correlates with behavioral patterns in stressed rats
- Authors: Khokhlov P.P.1, Tissen I.Y.1, Lebedev A.A.1, Bychkov E.R.1, Shabanov P.D.1
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Affiliations:
- Institute of Experimental Medicine
- Issue: Vol 16, No 4 (2018)
- Pages: 45-48
- Section: Articles
- URL: https://journal-vniispk.ru/RCF/article/view/11087
- DOI: https://doi.org/10.17816/RCF16445-48
- ID: 11087
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Abstract
The aim of this investigation was to reveal correlations between some behavioral patterns and orexin concentrations in the brain structures in rats after an acute psychoemotional stress. A group of rats was placed into a chamber with a tiger python for 25 mi nutes and then as a treatment these rats received orexin A or its antagonist SB-408124 administered intranasally for 7 days. Then the quantitive indexes of behavioral patterns in open field and elevated plus maze were registered. The orexin A level was investigated in the brain structures (amygdala, hippocampus, hypothalamus) of such rats by means of high-sensitive ELISA. The correlations were assessed with Spearman’s rank correlation test. As a result, a correlation between orexin A content in the amygdala and hypothalamus and a number of ambulations (line crossings) characterizing motor activity in open field test has been revealed. Therefore, the level of orexin A in the amygdala and hypothalamus demonstrates a direct and reverse link with locomotor activity of rats respectively. Also a correlation between orexin A level in the hippocampus and amygdala and the time of staying in the dark alleys of the elevated plus maze has been revealed. So, the content of orexin A in the hippocampus and amygdala reflects anxiety level of a rat. It is concluded there is a positive correlation between the orexin A content in the limbic structures of the brain and emotional behavior of rats.
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##article.viewOnOriginalSite##About the authors
Platon P. Khokhlov
Institute of Experimental Medicine
Author for correspondence.
Email: platonkh@list.ru
PhD, Senior Researcher, S.V. Anichkov Department of Neuropharmacology
Russian Federation, Saint PetersburgIlia Yu. Tissen
Institute of Experimental Medicine
Email: iljatis@mail.ru
PhD, Researcher, S.V. Anichkov Department of Neuropharmacology
Russian Federation, Saint PetersburgAndrei A. Lebedev
Institute of Experimental Medicine
Email: aalebedev-iem@rambler.ru
Dr. Biol. Sci. (Pharmacology), Leading Researcher, S.V. Anichkov Department of Neuropharmacology
Russian Federation, Saint PetersburgEugenii R. Bychkov
Institute of Experimental Medicine
Email: bychkov@mail.ru
PhD (Biochemistry), Head of the Laboratory, S.V. Anichkov Department of Neuropharmacology
Russian Federation, Saint PetersburgPetr D. Shabanov
Institute of Experimental Medicine
Email: pdshabanov@mail.ru
Dr. Med. Sci., Professor, Head, S.V. Anichkov Department of Neuropharmacology
Russian Federation, Saint PetersburgReferences
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