Email this article COMPARISON OF EFFICIENCY OF METHYLENE BLUE DRUGS FOR VISUALIZATIONOF LYMPHOTOUS WAYS IN THE EXPERIMENT
- Authors: Kovalenko VV1, Barinov OV1, Fufaev EE1, Dmitrochenko IV1, Kurakevich IV2, Ovcharenko AP1
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Affiliations:
- S. M. Kirov Military Medical Academy of the Russian Defense Ministry
- Branch N 1 "439 Military Hospital" of the Russian Defense Ministry
- Issue: Vol 39, No 1 (2020)
- Pages: 60-65
- Section: Articles
- URL: https://journal-vniispk.ru/RMMArep/article/view/33819
- DOI: https://doi.org/10.17816/rmmar33819
- ID: 33819
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Abstract
Objective. compare and evaluate the effectiveness of using methylene blue solutions to visualize lymphatic outflow pathways in laboratory animals (outbred rat). Materials and methods. In the experiment were used 16 female outbred rats aged about 40 days with an initial body weight of 180-220 g contained in standard vivarium conditions. For anesthesia was used “Zolazepam“ (Zoletil®100). Animals were randomized into 2 groups - 8 in each. The first group was injected subcutaneously in the left thigh with a 1% aqueous solution of methylene blue, the second group - solution of methylene blue with human serum albumin. Depending on the time of exposure, animals were divided randomly into 4 subgroups - 15, 30, 45 and 60 minutes. There was evaluated the effectiveness of marking the pathways of lymph outflow from the injection site. Results. As a result of the study, it was demonstrated that the use of a 1% methylene blue solution allows visualizing of the lymphatic outflow paths during drug exposure for 30 minutes. Staining of the lymph nodes of the next order occurs with an increase in exposure time. A solution of methylene blue in combination with human serum albumin stains the first node in the lymph drainage path 30 minutes after administration. The drug is characterized by slow migration from the first order lymph nodes (>60 minutes). Conclusion. The use of methylene blue solution in combination with serum albumin characterized by slow migration from the first order lymph nodes, which makes its use preferable in surgical interventions (8 figs, 1 table, bibliography: 14 refs).
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##article.viewOnOriginalSite##About the authors
V V Kovalenko
S. M. Kirov Military Medical Academy of the Russian Defense MinistrySaint Petersburg, Russia
O V Barinov
S. M. Kirov Military Medical Academy of the Russian Defense MinistrySaint Petersburg, Russia
E E Fufaev
S. M. Kirov Military Medical Academy of the Russian Defense MinistrySaint Petersburg, Russia
I V Dmitrochenko
S. M. Kirov Military Medical Academy of the Russian Defense MinistrySaint Petersburg, Russia
I V Kurakevich
Branch N 1 "439 Military Hospital" of the Russian Defense MinistryPrimorskiy Krai, Pogranichniy, Russia
A P Ovcharenko
S. M. Kirov Military Medical Academy of the Russian Defense MinistrySaint Petersburg, Russia
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