Determining the efficiency of low-intensity laser radiation exposure with a wavelength of 445 nm appliied to the gingival area according to the data from laser doppler flowmetry
- Authors: Romanenko N.V.1, Tulskikh E.V.1, Kirsanova N.M.1, Tarasenko S.V.1
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Affiliations:
- The First Sechenov Moscow State Medical University
- Issue: Vol 16, No 1 (2025)
- Pages: 38-46
- Section: Original Study Articles
- URL: https://journal-vniispk.ru/clinpractice/article/view/295968
- DOI: https://doi.org/10.17816/clinpract677229
- ID: 295968
Cite item
Abstract
BACKGROUND: Currently, the list of diode semiconductor lasers in surgical dental practice is expanded due to the development of the blue laser. The topical issue is the evaluation of the effects of laser radiation with a wavelength of 445 nm on the microcirculation of oral cavity mucosa. AIM: to assess the effects of 445 nm laser radiation with a 0.1 Watt power on the microcirculation and the lymphatic flow in the gingival area using the method of laser Doppler flowmetry. METHODS: The efficiency of laser radiation with a wavelength of 445 nm in the gingival area was determined by the data from laser Doppler flowmetry. Dynamic changes of the basal circulation and basal lymphatic flow in the area of the attached keratinized gingiva in the teeth of the mandible were evaluated in healthy volunteers: the numerical values obtained for the microcirculation were measured along with its variability and the coefficient of variation before and after laser therapy. Statistical analysis and visualization of the obtained data were performed using the R 4.4.2 statistical calculations medium. RESULTS: In a total of 20 volunteers, the method of laser Doppler flowmetry has shown a significant increase of the microcirculation values in the gingival tissues upon the exposure of the laser radiation with a wavelength of 445 nm to the gingiva (gum). CONCLUSION: An increase of the circulation rates in the gingival tissues upon the exposure of the low-intensity laser radiation with a wavelength of 445 nm indicates the presence of a therapeutic effect caused by the blue laser. The procedures of preventing the diseases of the parodontal tissues are recommended to be supplemented by photobiomodulation with using the blue laser technology at a power of 0.1 W.
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##article.viewOnOriginalSite##About the authors
Natalia V. Romanenko
The First Sechenov Moscow State Medical University
Author for correspondence.
Email: clematis@inbox.ru
ORCID iD: 0000-0002-5846-5578
SPIN-code: 4025-2805
Scopus Author ID: 245437
ResearcherId: 7805-2021
MD, PhD, Assistant Professor
Russian Federation, 11 Mozhaysky Val, Moscow, 121059Ekaterina V. Tulskikh
The First Sechenov Moscow State Medical University
Email: shchetininakatya@yandex.ru
ORCID iD: 0000-0001-7807-637X
Russian Federation, 11 Mozhaysky Val, Moscow, 121059
Natalia M. Kirsanova
The First Sechenov Moscow State Medical University
Email: nata.kirs14@yandex.ru
ORCID iD: 0009-0006-6348-1835
Russian Federation, 11 Mozhaysky Val, Moscow, 121059
Svetlana V. Tarasenko
The First Sechenov Moscow State Medical University
Email: prof_tarasenko@rambler.ru
ORCID iD: 0000-0001-8595-8864
SPIN-code: 3320-0052
MD, PhD, Professor
Russian Federation, 11 Mozhaysky Val, Moscow, 121059References
- Агафонова И.С. Применение вакуум-лазерной терапии в комплексном лечении хронического гингивита // Российский стоматологический журнал. 2024. Т. 28, № 1. С. 93–98. [Agafonova IS. Application of vacuum-laser therapy in complex treatment of chronic gingivitis. Russian journal of dentistry. 2024;28(1):93–98]. doi: 10.17816/dent607397 EDN: ATQMNX
- Karoussis IK, Kyriakidou K, Psarros C et al. Effects and Action Mechanism of Low-Level Laser Therapy (LLLT): Applications in periodontology. Dentistry. 2018;8(9):1000514. doi: 10.4172/2161-1122.1000514
- Светлакова Е.Н., Мандра Ю.В., Жегалина Н.М. Применение низкоинтенсивного лазерного излучения в комплексном лечении заболеваний пародонта // Проблемы стоматологии. 2010. № 5. С. 8–11. [Svetlakova EN, Mandra YuV, Zhegalina NM. Using of low intensity laser therapy in chronical gingivitis treatment. The Actual problems in dentistry. 2010;(5):8–11]. EDN: NRASVF
- Джиджавадзе С.В., Клинцова К.Ю., Овсиенко А.А. Применение лазерных технологий в комплексной противовоспалительной терапии гингивита: сборник трудов Национального конгресса с международным участием «Паринские чтения 2022», посвященного памяти профессора, заслуженного деятеля науки Республики Беларусь О.П. Чудакова. Минск, 5–6 мая. Минск, 2022. С. 124–128. [Dzhidzhavadze SV, Klintsova KYu, Ovsienko AA. Application of laser technologies in complex anti-inflammatory therapy of gingivitis: Collection of works of the National Congress with international participation “Parinsky Readings 2022”, dedicated to the memory of Professor, Honored Scientist of the Republic of Belarus O.P. Chudakov. Minsk, May 5-6. Minsk; 2022. Р. 124–128. (In Russ.)]
- Романенко Н.В., Тарасенко С.В., Крылова Ю.В. Применение диодного полупроводникового лазера при лечении протетического гингивита: материалы международной научно-практической конференции, посвященной памяти ученого С.Э. Кубаева. Самарканд, 25–26 февраля / под ред. Ж.А. Ризаева. Самарканд; 2022. С. 397–402. [Romanenko NV, Tarasenko SV, Krylova YuV. Application of a diode semiconductor laser in the treatment of prosthetic gingivitis: Proceedings of the international scientific and practical conference dedicated to the memory of the scientist S.E. Kubaev. Samarkand, February 25-26. Ed. by Zh.A. Rizaev. Samarkand; 2022. Р. 397–402. (In Russ.)]
- Manjunath Sh, Singla D, Singh R. Clinical and microbiological evaluation of the synergistic effects of diode laser with nonsurgical periodontal therapy: A randomized clinical trial. J Indian Society Periodontol. 2020;24(2):145–149. doi: 10.4103/jisp.jisp_101_19
- Sakurai Y, Yamaguchi M, Abiko Y. Inhibitory effect of low-level laser irradiation on LSP-stimulated prostaglandin E2 production and cyclooxygenase-2 in human gingival fibroblasts. Eur J Oral Sci. 2000;108(1):29–34. doi: 10.1034/j.1600-0722.2000.00783.x
- Nunez SC, Nogueira GE, Ribeiro MS, et al. He-Ne laser effects on blood microcirculation during wound healing: A method of in vivo study through laser Doppler flowmetry. Lasers Surg Med. 2004;35(5):363–368. doi: 10.1002/lsm.20109
- Moskvin SV. Low-level laser therapy in Russia: History, science and practice. Lasers Med Sci. 2017;8:56–65. doi: 10.15171/jlms.2017.11
- Convissar RА. Principles and practice of laser dentistry: Second edition. Mosby; 2015. 328 p.
- Мустафина Ф.К. Исследование показателей микроциркуляции для определения эффективности лечения катарального гингивита с использованием лазерного излучения у лиц молодого возраста: Дис. ... канд. мед. наук. Москва, 2005. 131 с. [Mustafina FK. Study of microcirculation parameters to determine the effectiveness of treatment of catarrhal gingivitis using laser radiation in young people [dissertation]. Moscow; 2025. 131 p. (In Russ.)] EDN: NPIMKB
- Кречина Е.К., Тарасова М.М. Гусева И.Е., и др. Клинико-функциональное обоснование лечения хронического генерализованного пародонтита методом ФДТ // Клиническая стоматология. 2024. Т. 27, № 4. С. 6–10. [Krechina EK, Tarasova MM, Guseva IE, et al. Clinical and functional rationale for the treatment of chronic generalized periodontitis using PDT. Clinical dentistry. 2024;27(4):6–10]. doi: 10.37988/1811-153X_2024_4_6 EDN: MJBVLD
- Palaia G, Pergolini D, D’Alessandro L, et al. Histological effects of an innovative 445 nm blue laser during oral soft tissue biopsy. Int J Environment Res Public Health. 2020;17(8):2651. doi: 10.3390/ijerph17082651
- Lusche I, Dirk C, Frentzen M, et al. Cavity disinfection with a 445 nm diode laser within the scope of restorative therapy: A pilot study. J Laser Med Sci. 2020;11(4):417–426. doi: 10.34172/jlms.2020.66
- Крупаткин А.И., Сидоров В.В. Функциональная диагностика состояния микроциркуляторно-тканевых систем: колебания, информация, нелинейность. Руководство для врачей. Изд. 2-е. Москва: Ленанд, 2016. 496 с. [Krupatkin AI, Sidorov VV. Functional diagnostics of the state of microcirculatory-tissue systems: Oscillations, information, nonlinearity. Manual for doctors. 2nd ed. Moscow: Lenand; 2016. 496 p. (In Russ.)]
- Canjãu S, Miron MI, Todea CD. Laser doppler flowmetry evaluation of gingival microcirculation recovery in gingivitis. Arch Balkan Med Union. 2015;50(3):354–359.
- Anders JJ, Lanzafame RJ, Arany PR. Low-level light/laser therapy versus photobiomodulation therapy. Photomed Laser Surg. 2015;33(4):183–184. doi: 10.1089/pho.2015.9848
- Романенко Н.В., Тарасенко С.В., Сережникова Н.Б., и др. Сравнительная оценка результатов биологического ответа слизистой оболочки полости рта на воздействие лазерным излучением с длиной волны 445 и 810 нм (экспериментальное исследование) // Клиническая стоматология. 2022. Т. 25, № 4. С. 137–143. [Romanenko NV, Tarasenko SV, Serezhnikova NB, et al. A comparative assessment of the results of the biological response of the oral mucosa on the effect of laser radiation with a wavelength of 445 nm and 810 nm. Clinical dentistry. 2022;25(4):137–143.] doi: 10.37988/1811-153X_2022_4_137 EDN: TRUZZG
- Малиновский Е.Л. Методическое пособие по эксплуатации магнито-ИК-светолазерного терапевтического аппарата «МИЛТА-Ф-5-01» (БИО). Москва: НПО Космического приборостроения, 2017. 352 с. [Malinovsky EL. Methodological manual for the operation of the magnetic-IK-light-laser therapeutic device “MILTA-F-5-01” (BIO). Moscow: NPO Kosmicheskogo priborostroeniya; 2017. 352 p. (In Russ.)]
- Сафонова Т.Н., Кинтюхина Н.П., Сидоров В.В., и др. Исследование микроциркуляции кровотока и лимфотока в коже век методом лазерной допплеровской флоуметрии // Вестник офтальмологии. 2017. Т. 133, № 3. С. 16–21. [Safonova TN, Kintukhina NP, Sidorov VV, et al. Microcirculatory blood and lymph flow examination in eyelid skin by laser Doppler flowmetry. Russian Annals of ophthalmology. 2017;133(3):16–21] doi: 10.17116/oftalma2017133316-21 EDN: ZATDRF
- Зюлькина Л.А., Сабаева М.Н., Иванов П.В., и др. Микроциркуляция тканей пародонта: причины нарушений и механизмы коррекции // Современные проблемы науки и образования. 2017. № 2. С. 1–9. [Zyulkina LA, Sabaeva MN, Ivanov PV, et al. Microcirculation of periodontal tissues: Causes of disorders and mechanisms of correction. Modern problems of science and education. 2017;(2):1–9. (In Russ.)]
- Yamamoto R, Amano K, Takahashi SW, et al. Changes in the microcirculation in periodontal tissue due to experimental peri-implantitis. J Oral Biosci. 2021;63(2):153–160. doi: 10.1016/j.job.2021.03.002
- Kerdvongbundit V, Sirirat M, Sirikulsathean A, et al. Blood flow and human periodontal status. Odontology. 2002;90:52–56. doi: 10.1007/s102660200008
- Романенко Н.В., Тарасенко С.В., Суворов А.Ю., и др. Экспериментальное обоснование безопасности применения лазерного излучения длиной волны 445±40 нм в стоматологической практике // Стоматология для всех. 2022. № 4. С. 28–33. [Romanenko NV, Tarasenko SV, Suvorov AY, et al. Experimental substantiation for the safety of the use of laser radiation with a wavelength of 445±40 nm in dental practice. Stomatology for all. 2022;(4):28–33]. doi: 10.35556/idr-2022-4(101)28-33 EDN: YGHGUI
- Kerdvongbundit V, Vongsavan N, Soo-Ampon S, et al. Microcirculation of the healthy human gingiva. Odontology. 2002;90:48–51. doi: 10.1007/s102660200007
- Kerémi B, Csempesz F, Vág J, et al. [Blood flow in marginal gingiva as measured with laser Doppler flowmetry. (In Hungarian)]. Fogorv Sz. 2000;93(6):163–168.
- Kouadio AA, Jordana F, Koffi NJ, et al. The use of laser Doppler flowmetry to evaluate oral soft tissue blood flow in humans: A review. Arch Oral Biol. 2018;86:58–71. doi: 10.1016/j.archoralbio.2017.11.009
- Laredo-Naranjo MA, Patiño-Marín N, Martínez-Castañón GA, et al. Identification of gingival microcirculation using laser doppler flowmetry in patients with orthodontic treatment: A longitudinal pilot study. Medicina. 2021;57:1081. doi: 10.3390/medicina57101081
- Firkova E, Bouka M. Laser Doppler flowmetry in the evaluation of periodontal health and disease. J IMAB. 2019;25(3):2599–2602. doi: 10.5272/jimab.2019253.2599
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