手术模拟子宫内膜异位症中子宫内膜样病灶中的谷胱甘肽过氧化物酶活性
- 作者: Razygraev A.V.1,2, Baziyan E.V.2, Polyanskikh L.S.2, Petrosyan M.A.2,3
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隶属关系:
- Saint Petersburg State Chemical and Pharmaceutical University
- The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott
- V.A. Almazov National Medical Research Center
- 期: 卷 70, 编号 2 (2021)
- 页面: 55-61
- 栏目: Original study articles
- URL: https://journal-vniispk.ru/jowd/article/view/52877
- DOI: https://doi.org/10.17816/JOWD52877
- ID: 52877
如何引用文章
详细
现实意义的题目已知编码抗氧化酶的基因的多态性、子宫内膜组织中过氧化氢酶和谷胱甘肽过氧化物酶活性的变化以及子宫内膜异位症的发展之间存在关系。某些形式的谷胱甘肽过氧化物酶被孕激素激活。同时,孕激素可有效确保子宫内膜异位灶的消退。评估子宫内膜样病灶中谷胱甘肽过氧化物酶活性与其退化程度之间的关系是有意义的。
目的是分析具有不同退化程度的模拟子宫内膜异位病灶中谷胱甘肽过氧化物酶的活性。
材料和方法。使用过氧化氢作为底物和5,5’-二硫代双(2-硝基苯甲酸)测定谷胱甘肽过氧化物酶活性以评估谷胱甘肽还原形式的损失的方法用于量化在实验室 Wistar 中模拟的子宫内膜异位病灶中的酶活性老鼠。测定是在37℃的组织匀浆中在基于 Tris-HCl 缓冲液的反应介质中进行的,该缓冲液含有乙二胺四乙酸盐和叠氮化钠(pH 8.5),存在 0.55 mM 谷胱甘肽和 0.192 mM 过氧化氢(孵育时间 - 40秒)。评价了谷胱甘肽过氧化物酶活性与病灶质量的相关性.
结果。以实验室大鼠为模型的子宫内膜异位病变通常以高、明确的谷胱甘肽过氧化物酶活性水平为特征。相同异位的谷胱甘肽过氧化物酶活性值与其在反应混合物中的蛋白质含量成正比。不同病灶谷胱甘肽过氧化物酶比活性值范围为每1mg蛋白质消耗谷胱甘肽2.43-6.45 μmol/min(n=7)。质量较低的病灶中(更强烈地受到回归)记录到较高的谷胱甘肽过氧化物酶活性 - Spearman 等级相关系数为–0.93 (p=0.0067)。
结论。用过氧化氢和5测定谷胱甘肽过氧化氢活性的方法,5,5’二硫代(2-硝基苯甲酸)适用于模拟子宫内膜异位症病灶的组织。病灶中谷胱甘肽过氧化物酶活性越高,其质量通常越小。结果与先前获得的催化酶活性数据相似,提示两种抗氧化剂酶均参与内膜源性退变机制。
作者简介
Aleksey Razygraev
Saint Petersburg State Chemical and Pharmaceutical University; The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott
编辑信件的主要联系方式.
Email: a.v.razygraev@gmail.com
ORCID iD: 0000-0002-0544-9398
SPIN 代码: 8623-7923
MD, PhD
俄罗斯联邦, 3 Mendeleevskaya line, Saint Petersburg, 199034; Saint PetersburgElena Baziyan
The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott
Email: waz2107gen@yandex.ru
ORCID iD: 0000-0001-7837-3315
SPIN 代码: 2232-9914
MD
俄罗斯联邦, Saint PetersburgLyudmila Polyanskikh
The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott
Email: polyanskikh-83@mail.ru
ORCID iD: 0000-0001-9994-8341
SPIN 代码: 2501-8880
MD
俄罗斯联邦, Saint PetersburgMariya Petrosyan
The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott; V.A. Almazov National Medical Research Center
Email: mariya@labpharm.spb.ru
ORCID iD: 0000-0001-7347-6104
SPIN 代码: 5329-5420
MD, PhD
俄罗斯联邦, Saint Petersburg; Saint Petersburg参考
- Ota H, Igarashi S, Sato N, et al. Involvement of catalase in the endometrium of patients with endometriosis and adenomyosis. Fertil Steril. 2002;78(4):804–809. doi: 10.1016/S0015-0282(02)03344-7
- Puy LA, Librach CL. A case-controlled analysis of catalase expression in endometriosis. Fertil Steril. 2001;76(3, suppl. 1):S149. doi: 10.1016/S0015-0282(01)02436-0
- Zarafshan SS, Salehi Z, Salahi E, et al. Polymorphism of catalase gene (CAT C-262T) in women with endometriosis. J Obstet Gynaecol. 2015;35(3):269–271. doi: 10.3109/01443615.2014.948402
- Razygraev AV, Petrosyan MA, Baziian EV, Polyanskikh LS. Activity of catalase in surgically induced endometrial-like lesions in rats. Journal of obstetrics and women’s diseases. 2019;68(6):57–63. (In Russ.). doi: 10.17816/JOWD68657-63
- Ota H, Igarashi S, Kato N, Tanaka T. Aberrant expression of glutathione peroxidase in eutopic and ectopic endometrium in endometriosis and adenomyosis. Fertil Steril. 2000;74:313–318. doi: 10.1016/S0015-0282(00)00638-5
- Torres WH. Biología de las especies de oxígeno reactivas. Mensaje Bioquimico. 2002;26:19–54. [cited: 2020 Dec 2]. Available from: http://static1.1.sqspcdn.com/static/f/1381323/18832492/1340082783193/Mensaje_Bioq02v26p019_Hansberg.pdf?token = %2BxnoB2oTZkYCHfk5sNZxT7gyf4Y%3D
- Petrosyan MA, Balashova NN, Polyanskikh LS, et al. Influence of progesterone analogs on endometrioid heterotopia in experimental model of endometriosis. Eksperimental’naya i Klinicheskaya Farmakologiya. 2018;81(7):14–19. (In Russ.). doi: 10.30906/0869-2092-2018-81-7-14-19
- Razygraev AV, Yushina AD, Titovich IA. Correction to: A method of measuring glutathione peroxidase activity in murine brain: Application in pharmacological experiment. Bulletin of experimental biology and medicine. 2018;165(4):589–592. (In Russ.). doi: 10.1007/s10517-018-4219-2
- Razygraev AV, Taborskaya KI, Petrosyan MA, Tumasova ZhN. Thiol peroxidase activities in rat blood plasma determined with hydrogen peroxide and 5,5’-dithio-bis(2-nitrobenzoic acid). Biomed Khim. 2016;62(4):431–438. (In Russ.). doi: 10.18097/PBMC20166204431
- Riddles PW, Blakeley RL, Zerner B. Ellman’s reagent: 5,5’-dithiobis(2-nitrobenzoic acid) — a reexamination. Analytical biochemistry. 1979;94(1):75–81. doi: 10.1016/0003-2697(79)90792-9
- Chesnokova LS, Voinova NE, Komkova AI, Lyanguzov AYu. Metody kolichestvennogo opredeleniia belka. In fermenty i nukleinovye kisloty. Ed. by S.N. Lyzlova, B.G. Vladimirov. Saint Petersburg; 1997. (In Russ.)
- r-project.org [Internet]. R Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna; 2017. [cited: 2017 May 5]. Available from: https://www.r-project.org/
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