WNT4、HOXA10、TWIST1基因在生殖器外子宫内膜异位症及子宫肌瘤发病机制中的作用分析
- 作者: Malysheva O.V.1,2, Molotkov A.S.1, Osinovskaya N.S.1,3, Shved N.Y.1,4, Yarmolinskaya M.I.1,3, Baranov V.S.1
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隶属关系:
- The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott
- Institute of Physiology named after I.P. Pavlov
- North-Western State Medical University named after I.I. Mechnikov
- City Hospital No. 40
- 期: 卷 70, 编号 3 (2021)
- 页面: 31-40
- 栏目: Original study articles
- URL: https://journal-vniispk.ru/jowd/article/view/64892
- DOI: https://doi.org/10.17816/JOWD64892
- ID: 64892
如何引用文章
详细
论证。子宫肌瘤和子宫内膜异位症是育龄妇女最常见的妇科疾病。许多数据表明,存在共同因素的发病机制,这些过度增殖条件。本研究旨在比较分析WNT4、HOXA10、TWIST1基因在子宫肌瘤及生殖器外子宫内膜异位症发生发展中的作用。
目的是评估子宫肌瘤、生殖器外子宫内膜异位症患者和对照组中多态变异rs7521902(WNT4基因) 和rs4721745(TWIST1基因)的频率;确定生殖器外子宫内膜异位症患者中HOXA10基因罕见等位变异的频率;研究这些基因在子宫肌瘤、生殖器外子宫内膜异位症及对照组子宫内膜中的表达特点。
材料与方法。采用实时聚合酶链反应方法研究WNT4和TWIST1基因在生殖器外子宫内膜异位症、子宫肌瘤和对照组中的多态性变异。对生殖器外子宫内膜异位症患者和对照组妇女进行了HOXА10二代基因外显子的测序。采用实时反转录聚合酶链反应方法分析WNT4、TWIST1、HOXA10基因在实验组患者子宫内膜标本中的表达情况。
结果。子宫肌瘤、生殖器外子宫内膜异位症患者和对照组中多态变异rs7521902(WNT4基因) 和rs4721745(TWIST1基因)的频率无显著性差异。在生殖器外子宫内膜异位症患者中未检测到少量HOXA10等位基因。WNT4基因在生殖器外子宫内膜异位症患者子宫内膜中的表达不受月经周期分期的影响,并与子宫肌瘤患者相比,WNT4基因在子宫内膜中的表达减少1.9倍。生殖器外子宫内膜异位症患者月经周期20-23天子宫内膜中HOXA10基因的表达较对照组显著降低。子宫肌瘤和生殖器外子宫内膜异位症患者子宫内膜中TWIST1基因的表达无变化。
结论。我们没有发现WNT4和TWIST基因的多态变异和HOXA10基因的少量变异与子宫肌瘤和生殖器外子宫内膜异位症之间的关联。WNT4和HOXA10基因在生殖器外子宫内膜异位症患者的子宫内膜中表达减少,而在子宫肌瘤患者中不表达。在这两种疾病中,所研究的基因在子宫内膜表达性质的变化显著不同。
作者简介
Olga Malysheva
The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott; Institute of Physiology named after I.P. Pavlov
编辑信件的主要联系方式.
Email: omal99@mail.ru
ORCID iD: 0000-0002-8626-5071
Cand. Sci. (Biol.)
俄罗斯联邦, Saint PetersburgArseny Molotkov
The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott
Email: arseny.molotkov@gmail.com
ORCID iD: 0000-0003-3433-3092
SPIN 代码: 6359-6472
Cand. Sci. (Med.)
俄罗斯联邦, Saint PetersburgNatalya Osinovskaya
The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott; North-Western State Medical University named after I.I. Mechnikov
Email: natosinovskaya@mail.ru
ORCID iD: 0000-0001-7831-9327
SPIN 代码: 3190-2307
Cand. Sci. (Biol.)
俄罗斯联邦, Saint PetersburgNatalya Shved
The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott; City Hospital No. 40
Email: natashved@mail.ru
ORCID iD: 0000-0001-6354-9226
SPIN 代码: 8276-1720
Cand. Sci. (Biol.)
俄罗斯联邦, Saint PetersburgMaria Yarmolinskaya
The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott; North-Western State Medical University named after I.I. Mechnikov
Email: m.yarmolinskaya@gmail.com
ORCID iD: 0000-0002-6551-4147
Scopus 作者 ID: 7801562649
Researcher ID: P-2183-2014
MD, Dr. Sci. (Med.), Professor, Professor of the Russian Academy of Sciences
俄罗斯联邦, Saint PetersburgVladislav Baranov
The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott
Email: baranov@vb2475.spb.edu
ORCID iD: 0000-0002-6518-1207
SPIN 代码: 9196-7297
MD, Dr. Sci. (Med.), Professor, Corresponding Member of the Russian Academy of Sciences, Honored Scientist of the Russian Federation
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