电邮这篇文章 The value of vaginal lactobacilli in the restoration of vaginal microbiocenosis in women in the early postpartum period, depending on the method of delivery
- 作者: Dadayeva D.G.1, Budilovskaya O.V.2,3, Krysanova A.A.2,3, Khusnutdinova T.A.4, Savicheva A.M.2,3, Kogan I.Y.5
-
隶属关系:
- The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott
- Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott
- Saint Petersburg State Pediatric Medical University
- Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott, Saint Petersburg State Pediatric Medical University
- Saint Petersburg State University
- 期: 卷 70, 编号 4 (2021)
- 页面: 15-23
- 栏目: Original study articles
- URL: https://journal-vniispk.ru/jowd/article/view/66711
- DOI: https://doi.org/10.17816/JOWD66711
- ID: 66711
如何引用文章
开放存取
##reader.subscriptionAccessGranted##
订阅存取
详细
BACKGROUND: Despite numerous studies of the vaginal microbiota, there is still a lack of knowledge regarding its restoring dynamics in the early postpartum period. The condition of the vaginal microflora during pregnancy plays a key role in maintaining the physiological microbiocenosis of the birth canal and creating conditions for the normal course of pregnancy, the establishment of an infant’s intestinal microbiota, and the further development of the child.
AIM: The aim of this study was to estimate the role of certain types of lactobacilli in restoring the vaginal microbiota in women in the early postpartum period, depending on the method of delivery.
MATERIALS AND METHODS: We examined 150 women at 38-41 weeks of gestation. The clinical material for the study was vaginal discharge before and after delivery. To determine the species of lactobacilli and other microorganisms in the clinical material, we used quantitative real-time PCR.
RESULTS: Before delivery, lactobacilli were found in vaginal discharge in 144 out of 150 women (96.0%), their number in the majority being more than 106 GE. In the postpartum period, lactobacilli were found in 66/128 (51.5%) cases, while prevailing in women after vaginal delivery in 50/65 (76.9%) cases (4.6±1.6, p = 0.000000). Among the dominant species of lactobacilli was L. crispatus, found in vaginal discharge before delivery, which most often affects the recovery of the vaginal microbiota in the postpartum period (29 out of 61 women, 47.5%). L. iners detected in the lochia predisposes to the violation of uterine involution in the early postpartum period (p = 0.03).
CONCLUSIONS: Normal vaginal microbiota in the postpartum period is restored more quickly in women after vaginal delivery. Our study confirms that L. crispatus and L. iners play a major role in restoring the vaginal microbiota in the postpartum period.
作者简介
Dzhamilya Dadayeva
The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott
Email: djamilya2010@mail.ru
ORCID iD: 0000-0002-1190-0149
MD
俄罗斯联邦, 3 Mendeleevskaya Line, Saint Petersburg, 199034Olga Budilovskaya
Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott; Saint Petersburg State Pediatric Medical University
Email: o.budilovskaya@gmail.com
ORCID iD: 0000-0001-7673-6274
SPIN 代码: 7603-6982
MD
俄罗斯联邦, 3 Mendeleevskaya Line, Saint Petersburg, 199034; Saint PetersburgAnna Krysanova
Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott; Saint Petersburg State Pediatric Medical University
Email: krusanova.anna@mail.ru
ORCID iD: 0000-0003-4798-1881
SPIN 代码: 2438-0230
MD, Researcher, Laboratory of Microbiology, Assistant of the Department of Clinical Laboratory Diagnostics of the Faculty of Postgraduate and Additional Professional Education
俄罗斯联邦, 3 Mendeleevskaya Line, Saint Petersburg, 199034; Saint PetersburgTatyana Khusnutdinova
Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott, Saint Petersburg State Pediatric Medical University
Email: HusnutdinovaT@yandex.ru
ORCID iD: 0000-0002-2742-2655
SPIN 代码: 9533-9754
Researcher. The Laboratory of Microbiology; Assistant. The Department of Clinical Laboratory Diagnostics, the Faculty of Postgraduate and Additional Professional Education
俄罗斯联邦, 3 Mendeleevskaya Line, Saint Petersburg, 199034; Saint PetersburgAlevtina Savicheva
Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott; Saint Petersburg State Pediatric Medical University
Email: savitcheva@mail.ru
ORCID iD: 0000-0003-3870-5930
SPIN 代码: 8007-2630
Scopus 作者 ID: 6602838765
MD, Dr. Sci. (Med.), Professor, Honored Scientist of the Russian Federation, Head of the Laboratory of Microbiology, Head of the Department of Clinical Laboratory Diagnostics of the Faculty of Postgraduate and Additional Professional Education
俄罗斯联邦, 3 Mendeleevskaya Line, Saint Petersburg, 199034; Saint PetersburgIgor Kogan
Saint Petersburg State University
编辑信件的主要联系方式.
Email: ikogan@mail.ru
ORCID iD: 0000-0002-7351-6900
SPIN 代码: 6572-6450
Scopus 作者 ID: 56895765600
MD, Dr. Sci. (Med.), Professor, Corresponding Member of RAS, Director
俄罗斯联邦, Saint Petersburg参考
- Marco J, Coolen MJL, Post E, et al. Characterization of microbial communities found in the human vagina by analysis of terminal restriction fragment length polymorphisms of 16S rRNA genes. Appl Environ Microbiol. 2005;71(12):8729–8737. doi: 10.1128/AEM.71.12.8729-8737.2005
- Pavlova SI, Kilic AO, Kilic SS, et al. Genetic diversity of vaginal lactobacilli from women in different countries based on 16S rRNA gene sequences. J Appl Microbiol. 2002;92(3):451–459. doi: 10.1046/j.13652672.2002.01547.x
- Giakoumelou S, Wheelhouse N, Cuschieri K, et al. The role of infection in miscarriage. Hum Reprod Update. 2016;22(1):116–133. doi: 10.1093/humupd/dmv041
- Song Y, Kato N, Matsumiya Y, et al. Identification of and hydrogen peroxide production by fecal and vaginal lactobacilli isolated from Japanese women and newborn infants. J Clin Microbiol. 1999;37:3062–3064. doi: 10.1128/JCM.37.9.3062-3064.1999
- Ravel J, Gajer P, Abdo Z, et al. Vaginal microbiome of reproductive-age women. Proc Natl Acad Sci USA. 2011;108(Suppl 1):4680–4687. doi: 10.1073/pnas.1002611107
- Antonio MAD, Hawes SE, Hillier SL. The identification of vaginal Lactobacillus species and the demographic and microbiologic characteristics of women colonized by these species. J Infect Dis. 1999;180:1950–1956. doi: 10.1086/315109
- Rampersaud R, Planet PJ, Randis TM, et al. Inerolysin, a cholesterol-dependent cytolysin produced by Lactobacillus iners. J BacterioL. 2011;193:1034–1041. doi: 10.1128/JB.00694-10
- Macklaim JM, Fernandes AD, Di Bella JM, et al. Comparative meta-RNA-seq of the vaginal microbiota and differential expression by Lactobacillus iners in health and dysbiosis. Microbiome. 2013;1:12. doi: 10.1186/2049-2618-1-12
- Dobrokhotova YE, Zatikyan NG. Sovremennye predstavleniya o mekhanizmakh razvitiya disbioza vlagalishcha. Akusherstvo, ginekologiya i reproduktsiya. 2008;(3):3–6. (In Russ.)
- Martikaynen ZM. Korinebakterii zhenskikh genitaliy. [dissertation abstract]. Saint Petersburg; 1996. (In Russ.). [cited 2021 May 24]. Available from: http://earthpapers.net/preview/486600/a#?page = 9
- MacIntyre DA, Chandiramani M, Lee YS, et al. The vaginal microbiome during pregnancy and the postpartum period in a European population. SciRep. 2015;5:89–88. doi: 10.1038/srep08988
- Kolesaeva ZhJu. Mikrobiocenoz vlagalishha u rodil’nic posle estestvennyh rodov i operacii kesarevo sechenija. [dissertation abstract]. Saint Petersburg; 2012. (In Russ.). [cited 2021 May 24]. Available from: https://viewer.rusneb.ru/ru/000199_000009_005009137?page = 1&rotate = 0&theme = white
- Mel’nikova TA. Osobennosti techenija beremennosti i rodov u zhenshhin s razlichnoj stepen’ju kolonizacii nizhnego otdela reproduktivnogo trakta Ureaplasma urealiticum. [dissertation abstract]. Permian; 2020. (In Russ.). [cited 2021 May 24]. Available from: https://www.dissercat.com/content/osobennosti-techeniya-beremennosti-i-rodov-u-zhenshchin-s-razlichnoi-stepenyu-kolonizatsii
- Morozova NA, Holodnjak TI, Ovsjanik MA. Sovremennyj vzgljad na problem subinvoljucii matki. Novosti mediciny i farmacii. 2009;21(301):36–42. (In Russ.)
- Kim MJ, Romero R, Gervasi MT, et al. Widespread microbial invasion of the chorioamniotic membranes is a consequence and not a cause of intra-amniotic infection. Lab Invest. 2009;89(8):924–936. doi: 10.1038/labinvest.2009.49
- Sinyakova AA, Shipitsyna EV, Budilovskaya OV, et al. Anamnestic and microbiological predictors of miscarriage. Journal of Obstetrics and Women’s Diseases. 2019;68(2):59–70. doi: 10.17816/JOWD68259-70
- Petricevic L, Domig KJ, Nierscher FJ, et al. Characterisation of the vaginal Lactobacillus microbiota associated with preterm delivery. Sci Rep. 2014;4:5136. doi: 10.1038/srep05136.
补充文件
