Technology of liquid chromatographic serum spectral images fixation in the diagnosis of colonic mucosal dysplasia
- Authors: Alekseeva OP1, Kolodey EN1
-
Affiliations:
- Nizhny Novgorod regional clinical hospital named after Semashko
- Issue: Vol 98, No 2 (2017)
- Pages: 272-277
- Section: Clinical experiences
- URL: https://journal-vniispk.ru/kazanmedj/article/view/6226
- DOI: https://doi.org/10.17750/KMJ2017-272
- ID: 6226
Cite item
Full Text
Abstract
Aim. To demonstrate the possibility of use of technology of liquid chromatographic serum spectral images fixing for the diagnosis of colonic epithelial dysplasia in patients with ulcerative colitis.
Methods. 49 patients with ulcerative colitis were examined. Colonoscopy with Mayo index evaluation and biopsy of the intestinal mucosa were carried out using OLYMPUS CV-170 device (Japan). Biopsies were taken from the most affected areas of the colonic mucosa (from 3 to 10 biopsies). Morphological study included an assessment of histological activity of ulcerative colitis and colonic mucosal dysplasia. The results of histological studies were evaluated by two experts. High performance liquid chromatography of serum was performed by a standard technique in the chromatograph «Milichrom A-02» (CJSC «EcoNova», Novosibirsk). Statistical analysis of serum chromatograms was performed with the use of chromatograph combined with a PC, the end result was construction of spectral images of the disease.
Results. In 13 patients with ulcerative colitis the results of histological examination revealed various degrees of dysplasia. The set of spectral images of different patients, obtained in the processing of chromatograms, composed the diagnostic cloud of a pathological condition. The study revealed complete differentiation of liquid-chromatographic spectral images of serum of patients with ulcerative colitis with and without colonic mucosal dysplasia according to the results of multivariate cluster analysis. The diagnostic accuracy of the method was 88%.
Conclusion. The possibility of using the technology of construction and analysis of liquid-chromatographic spectral images of serum for the diagnosis of colonic epithelial dysplasia in patients with ulcerative colitis was demonstrated.
About the authors
O P Alekseeva
Nizhny Novgorod regional clinical hospital named after Semashko
Author for correspondence.
Email: al_op@mail.ru
Nizhny Novgorod, Russia
E N Kolodey
Nizhny Novgorod regional clinical hospital named after Semashko
Email: al_op@mail.ru
Nizhny Novgorod, Russia
References
- Krok K.L., Lichtenshstein G.R. Colorectal cancer in inflammatory bowel disease. Curr. Opin. Gastroenterol. 2004; 20: 43-48. doi: 10.1097/00001574-200401000-00009.
- Киркин Б.В., Капуллер Л.Л., Маят К.Е. и др. Рак толстой кишки у больных неспецифическим язвенным колитом. Клин. мед. 1988; 66 (9): 108-113.
- Воробьёв Г.И., Михайлова Т.Л., Костенко Н.В. Достижимы ли удовлетворительные результаты хирургического лечения язвенного колита? Колопроктология. 2006; (2): 34-43.
- Захарова Е.Ю., Воскобоева Е.Ю., Байдакова Г.В. и др. Наследственные болезни обмена веществ. В кн.: Клиническая лабораторная диагностика. Национальное руководство. Под ред. В.В. Долгова. М.: ГЭОТАР-Медиа. 2012; 1: 719-735.
- Фёдорова Г.А., Кожанова Л.А., Азарова И.Н. Применение микроколоночной высокоэффективной жидкостной хроматографии в медицине. Вестн. восстановительной мед. 2008; (2): 47-50.
- Ивашкин В.Т., Шелыгин Ю.А., Абдулганиева Д.И. и др. Рекомендации Российской гастроэнтерологической ассоциации и Ассоциации колопроктологов России по диагностике и лечению взрослых больных язвенным колитом. Рос. ж. гастроэнтерол., гепатол., колопроктол. 2015; 25 (1): 48-65.
- Saverymuttu S.H., Camilleri M., Rees H. et al. Indium 111-granulocyte scanning in the assessment of disease extent and disease activity in inflammatory bowel disease. A comparison with colonoscopy, histology, and fecal indium 111-granulocyte excretion. Gastroenterology. 1986; 90: 1121-1128. doi: 10.1016/0016-5085(86)90376-8.
- Thomas T., Abrams K.A., Robinson R.J., Mayberry J.F., Cancer risk of low-grade dysplasia in chronic ulcerative colitis: a systematic review and meta-analysis. Aliment. Pharmacol. Ther. 2007; 25: 657-668. doi: 10.1111/j.1365-2036.2007.03241.x.
- Барам Г.И. Хроматограф «Милихром А-02». Определение веществ с применением баз данных «ВЭЖХ-УФ». Новосибирск. 2005; 64 с.
- Насонов С.В., Игнатьев А.А., Казаковцев А.В., Челышев И.В. Программа для обработки спектров и создания экспертных диагностических систем «DIASTAT». Свидетельство об официальной регистрации программы для ЭВМ №2007611453 от 2007.
- Baars J.E., Woude C.J. So where is all the cancer? In: Clinical dilemmas in inflammatory bowel disease: new challenges. Ed. P. Irving et al. 2nd ed. Chichester, West Sussex: Wiley-Blackwell. 2011; 222-224.
- Hurlstone D.P., Sanders D.S., Lobo A.J. et al. Indigo carmine-assisted high-magnification chromoscopic colonoscopy for the detection and characterization of intraepithelial neoplasia in ulcerative colitis: a prospective evaluation. Endoscopy. 2005; 37: 1186-1192. doi: 10.1055/s-2005-921032.
- Kiesslich R., Goetz M., Lammersdorf K. et al. Chromoscopyguided endomicroscopy increases. The diagnostic yield of intraepithelial neoplasia in ulcerative colitis. Gastroenterology. 2007; 132: 874-882. doi: 10.1053/j.gastro.2007.01.048.
- Brentnall T.A., Crispin D.A., Rabinovitch P.S. et al. Mutations in the p53 gene: an early marker of neoplastic progression in ulcerative colitis. Gastroenterology. 1994; 107: 369-378. doi: 10.1016/0016-5085(94)90161-9.
- Lashner B.A. Cancer in inflammatory bowel disease. In: The clinician’s guide to inflammatory bowel disease. Ed. G.R. Lichtenstein. Thorofare, New Jersey: Slack Inc. 2003; 113-123.
- Насонов С.В., Алексеева О.П. Диагностика и дифференциальная диагностика заболеваний желудка с использованием высокоэффективной жидкостной хроматографии сыворотки крови. Мед. альманах. 2008; (2): 30-33.
Supplementary files
