电邮这篇文章 体外研究孕激素对糖蛋白- P活性的影响
- 作者: Erokhina P.D.1, Abalenikhina Y.V.1, Shchulkin A.V.1, Chernykh I.V.1, Popova N.M.1, Slepnev A.A.1, Yakusheva E.N.1
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隶属关系:
- Ryazan State Medical University
- 期: 卷 28, 编号 2 (2020)
- 页面: 135-142
- 栏目: Original study
- URL: https://journal-vniispk.ru/pavlovj/article/view/34901
- DOI: https://doi.org/10.23888/PAVLOVJ2020282135-142
- ID: 34901
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详细
研究现实性。糖蛋白- P（Pgp，ABCB1）是一种参与药物药代动力学和肿瘤细胞化疗耐药发展的转运蛋白。
目的：目的：研究黄体酮对体外培养的人小肠上皮细胞模型中Pgp活性的影响。
材料与方法：对Caco-2细胞系进行了研究。在特殊的Transwell体系中，通过非索非那定转运评价Pgp的活性。高效液相色谱法测定非索非那定的浓度。采用免疫酶测定法测定Pgp的含量。我们进行了四个系列的实验：对照组细胞在不添加任何物质的清洁运输介质中孵育；预孵育3天，10 mcmol / L浓度利福平对Pgp活性及合成的影响（诱导控制）；孕酮在1、10和100 mol / L预潜伏30分钟时对Pgp活性的影响；孕酮在1、10、100 mcmol / L预潜伏3天后对Pgp活性及合成的影响。
结果：黄体酮与细胞潜伏30 分钟时，浓度为1和10的黄体酮对Pgp的活性没有显著影响，但浓度为100的黄体酮会降低转运蛋白的活性。
将Caco-2细胞与黄体酮浓度分别为1，10和100微米的水杨酸潜伏3天后，Pgp的活性没有变化。与对照指标相比，孕酮在潜伏3天后，浓度为100微米的孕酮可显著提高肠上皮细胞Pgp的合成，提高114.3%，但在剩余的使用浓度（1和10微米）中效果不显著。
结论：体外实验中，在Caco-2细胞系细胞中，孕激素浓度为100微米时，对Pgp活性有直接抑制作用，但在潜伏3天后，孕酮增加了转运蛋白的合成，使其抑制活性减弱。
作者简介
Pelageya Erokhina
Ryazan State Medical University
Email: p34-66@yandex.ru
ORCID iD: 0000-0003-4802-5656
Student
俄罗斯联邦, RyazanYulia Abalenikhina
Ryazan State Medical University
Email: p34-66@yandex.ru
ORCID iD: 0000-0003-0427-0967
SPIN 代码: 4496-9027
Researcher ID: L-8965-2018
PhD in Biological Sciences, Assistant Professor of the Department of Biological Chemistry
俄罗斯联邦, RyazanAlexey Shchulkin
Ryazan State Medical University
Email: p34-66@yandex.ru
ORCID iD: 0000-0003-1688-0017
SPIN 代码: 2754-1702
Researcher ID: N-9143-2016
MD, PhD, Assistant Professor of the Department of Pharmacology with a Course of Pharmacy of Continuing Professional Education Faculty
俄罗斯联邦, RyazanIvan Chernykh
Ryazan State Medical University
Email: p34-66@yandex.ru
ORCID iD: 0000-0002-5618-7607
SPIN 代码: 5238-6165
Researcher ID: R-1389-2017
PhD in Biological Sciences, Head of the Department of Pharmaceutical Chemistry
俄罗斯联邦, RyazanNatalia Popova
Ryazan State Medical University
编辑信件的主要联系方式.
Email: p34-66@yandex.ru
ORCID iD: 0000-0002-5166-8372
SPIN 代码: 7553-9852
Researcher ID: B-1130-2016
MD, PhD, Assistant Professor of the Department of Pharmacology with a Course of Pharmacy of Continuing Professional Education Faculty
俄罗斯联邦, RyazanAlexandr Slepnev
Ryazan State Medical University
Email: p34-66@yandex.ru
ORCID iD: 0000-0003-0696-6554
PhD in Biological Sciences, Assistant Professor of the Department of Pharmacology with a Course of Pharmacy of Continuing Professional Education Faculty
俄罗斯联邦, RyazanElena Yakusheva
Ryazan State Medical University
Email: p34-66@yandex.ru
ORCID iD: 0000-0001-6887-4888
SPIN 代码: 2865-3080
Researcher ID: T-6343-2017
MD, PhD, Professor, Head of the Department of Pharmacology with a Course of Pharmacy of Continuing Professional Education Faculty
俄罗斯联邦, Ryazan参考
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