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Post a Comment Biological tissue reaction to the intracorporeally-polymerising implant used in a minimally invasive “puncture-infusion-seal” method of the inguinal canal
- Authors: Trunin E.M.1, Mowlabucus M.J.1, Murt L.L.1, Smirnov A.A.1, Tatarkin V.V.1, Rybakov V.A.1
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Affiliations:
- North-Western State Medical University named after I.I. Mechnikov
- Issue: Vol 10, No 2 (2018)
- Pages: 99-106
- Section: Articles
- URL: https://journal-vniispk.ru/vszgmu/article/view/9287
- DOI: https://doi.org/10.17816/mechnikov201810299-106
- ID: 9287
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Abstract
The article focuses on the study of the biological properties of a synthetic intracorporeally-polymerising implant, designed to be used in a minimally invasive “puncture-infusion-seal” technique under experimental study for the treatment of reducible inguinal hernias. The method is innovative in the field of herniology and surgery in general for its minimal invasiveness. Two types of experiments were conducted to investigate the response of live tissues of the body to the implant as a foreign body (on rats) and the impact of the implant on the male reproductive system of rabbits.
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##article.viewOnOriginalSite##About the authors
E. M. Trunin
North-Western State Medical University named after I.I. Mechnikov
Author for correspondence.
Email: mjaweed10@hotmail.com
Department of Operative and Clinical Surgery with Topographical Anatomy
Russian Federation, Saint PetersburgM. J. Mowlabucus
North-Western State Medical University named after I.I. Mechnikov
Email: mjaweed10@hotmail.com
Department of Operative and Clinical Surgery with Topographical Anatomy
Russian Federation, Saint PetersburgL. L. Murt
North-Western State Medical University named after I.I. Mechnikov
Email: mjaweed10@hotmail.com
Department of Operative and Clinical Surgery with Topographical Anatomy
Russian Federation, Saint PetersburgA. A. Smirnov
North-Western State Medical University named after I.I. Mechnikov
Email: mjaweed10@hotmail.com
Department of Operative and Clinical Surgery with Topographical Anatomy
Russian Federation, Saint PetersburgV. V. Tatarkin
North-Western State Medical University named after I.I. Mechnikov
Email: mjaweed10@hotmail.com
Department of Operative and Clinical Surgery with Topographical Anatomy
Russian Federation, Saint PetersburgV. A. Rybakov
North-Western State Medical University named after I.I. Mechnikov
Email: mjaweed10@hotmail.com
Department of Operative and Clinical Surgery with Topographical Anatomy
Russian Federation, Saint PetersburgReferences
- Должиков А.А., Колпаков А.Я., Ярош А.Л., и др. Гигантские клетки инородных тел и тканевые реакции на поверхности имплантатов // Курский научно-практический вестник «Человек и его здоровье». - 2017. - Т. 3. - C. 86-94. [Dolzhikov AA, Kolpakov AY, Yarosh AL, et al. Giant foreign body cells and tissue reactions on the surface of implants. Kurskii nauchno-prakticheskii vestnik “Chelovek i ego zdorov’e”. 2017;(3):86-94. (In Russ.)]. doi: 10.21626/vestnik/2017-3/15.
- Жебровский В.В. Общие принципы лечения грыж живота [Электронный ресурс]. URL: http://www.medlinks.ru/article.php. [Zhebrovskiy VV. Obshie principi lechenia grizh zhivota. (In Russ.)]
- Касаткина Т.Б., Капланский А.С. Этика экспериментальных исследований на животных в космической биологии и медицине // Авиакосмическая и экологическая медицина. - 2000. - Т. 34. - № 2. - C. 17-21. [Kasatkina TB, Kaplansky AS. Ethics of space life sciences research with animals. Aviakocmicheskaya i ekologicheskaya meditsina. 2000;34(2):17-21. (In Russ.)]
- Резниченко Н.Ю., Резниченко Ю.Г., Веретельник А.В., и др. Коррекция проявлений физиологического и фотостарения с использованием янтарной кислоты в составе инъекционного имплантата «гиалуаль» // Украинский журнал дерматологии, венерологии, косметологии. - 2010. - № 36 (1). - C. 64-69. [Reznichenko NYu, Reznichenko YuG, Veretelnik AV, et al. The correction of physiologic and photoaging with the use of amber acid in injective implant Hyalual. Ukrainian Journal of Dermatology, Venerology, Cosmetology. 2010;36(1):64-69. (In Russ.)]
- Anderson FA. Amended final report on the safety assessment of polyacrylamide and acrylamide residues in cosmetics. Int J Toxicol. 2005;24Suppl 2:21-50.
- Anderson JM, Rodriguez A, Chang DT. Foreign body reaction to biomaterials. Seminars in Immunology. 2008;20(2):86-100. doi: 10.1016/j.smim.2007.11.004.
- Feldman AT, Wolfe D. Tissue Processing and Hematoxylin and Eosin Staining. In: Day C. (eds) Histopathology. Methods in Molecular Biology (Methods and Protocols). Vol. 1180. New York: Humana Press; 2014.
- Kastellorizios M, Tipnis N, Burgess DJ. Foreign body reaction to subcutaneous implants. Advances in Experimental Medicine and Biology. 2015;865:93-108. doi: 10.1007/978-3-319-18603-0_6.
- Liliana MR, Daniela ED, Eleonora AD. Bioethical dilemmas in using animal in medical research. Challenges and opportunities. Rom J Morphol Embryol. 2015;56(3):1227-1231.
- Punchard NA, Whelan CJ, Adcock I. The Journal of Inflammation. Journal of Inflammation (London, England). 2004;1:1. doi: 10.1186/1476-9255-1-1.
- Til HP, Falke HE, Prinsen MK, Willems MI. Acute and subacute toxicity of tyramine, spermidine, spermine, putrescine and cadaverine in rats. Food and Chemical Toxicology. 1997;3-4(35):337-348.
- Valanzo A. Rules of good practice in the care of laboratory animals used in biomedical research. Ann 1st Super Sanità. 2004;40(2):201-203.
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Fig. 1. Excised tissue in firm contact with the implant: 1 — nerve, 2 — implant, 3 — muscle
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Fig. 2. Excised tissue in firm contact with the implant: 1 — implant, 2 — muscle
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Fig. 3. Period of 3 days. Acute inflammation: 1 — muscle; 2 — leucocyte margin around the implant; 3 — implant. Magnification ×40. Hematoxilin-Eozin stain
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Fig. 4. Period of 7 days (1 week). Presence of productive inflammation belt (within the zone of contact between tissue and implant): 1 — implant, 2 — productive inflammation belt, 3 — acute inflammatory response cells (neutrophils, lymphocytes, macrophages), 4 — muscle. Magnification ×40. Hematoxilin-Eozin stain
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Fig. 5. Period of 14 days (2 weeks). Appearance of giant foreign body cells: 1 — Neutrophils and lymphocytes; 2 — implant; 3 — giant foreign body cells; 4 — muscle. Magnification ×40. Hematoxilin-Eozin stain
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Fig. 6. Period of 21 days (3 weeks). Аctive aseptic inflammatory reaction: 1 — fascia (active aseptic inflammatory reaction over the surface of fascia in contact with the implant), 2 — acute inflammatory response cells (neutrophils, lymphocytes, macrophages), 3 — implant, 4 — belt within the zone of contact between tissue and foreign body (fusion between organic and nonorganic substrate as a result of covalent bond formation and invasion of the implant by acute inflammatory response cells), 5 — muscle. Magnification ×20. Hematoxilin-Eozin stain
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Fig. 7. Period of 28 days (1 month). Response of the organism to foreign body: 1 — implant, 2 — acute inflammatory response cells, 3 — fascia, 4 — muscle. Magnification ×40. Hematoxilin-Eozin stain
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Fig. 8. Period of 60 days (2 months). Absence of exudative inflammation. Formation of granulation tissue (productive inflammation – fibroblasts and blood vessels) resulting in the formation of a thin fibrous capsule, which forms a border between tissue and implant: 1 — implant, 2 — muscle, 3 — moderate productive inflammation, 4 — more significant productive inflammation. Magnification ×10. Hematoxilin-Eozin stain
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Fig. 9. Period of 60 days (2 months). Growth of connective tissue into the porous structure of the implant (occurs as a result of micro air bubbles, which are formed during the mixing process of the main components of the implant): 1 — connective tissue, 2 — implant. Magnification ×20. Hematoxilin-Eozin stain
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Fig. 10. Period of 90 days (3 months). Formation of a thin connective tissue capsule, which forms a border between implant and surrounding tissue: 1 — muscle, 2 — connective tissue capsule, 3 — implant. Magnification ×40. Hematoxilin-Eozin stain
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Fig. 11. Period of 120 days (4 months). Demarcation of implant from tissues in contact: 1 — muscle, 2 — fascia, 3 — connective tissue capsule, 4 — implant. Magnification ×10. Hematoxilin-Eozin stain
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Fig. 12. Period of 150 days (5 months). Encapsulation of the implant: 1 — connective tissue capsule, 2 — implant, 3 — muscle. Magnification ×20. Hematoxilin-Eozin stain
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Fig. 13. Period of 180 days (6 months). Mature fibrous tissue capsule: 1 — implant, 2 — fusion of fibrous tissue and polymer, 3 — mature fibrous tissue capsule. Magnification ×40. Hematoxilin-Eozin stain
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Fig. 14. Period of 270 days (9 months). Fibrous tissue layer firmly attaches the implant to tissue in contact: 1 — implant, 2 — connective tissue (fascia), 3 — nerve, 4 — fibrous tissue layer. Magnification ×40. Hematoxilin-Eozin stain
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Fig. 15. Period of 360 days (12 months). Firm contact between implant and sub-lying tissue: 1 — implant, 2 — fibrous tissue capsule, 3 — muscle, 4 — fascia. Magnification ×40. Hematoxilin-Eozin stain
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Fig. 16. Measurement of the length of the organo-complex consisting of testicles and spermatic cords. (Implant around the left spermatic cord)
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Fig. 17. Measurement of the width of the organo-complex consisting of testicles and spermatic cords. (Implant around the left spermatic cord)
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Fig. 18. Tissue of the right testicle (control). Magnification ×10. Hematoxilin-Eozin stain
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Fig. 19. Tissue of the left testicle without signs of inflammation, necrosis, fibrosis. Magnification ×10. Hematoxilin-Eozin stain
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