Synthesis of exo-β-glucosaminidase BY FUNGUS Penicillium sp. IB-37-2
- 作者: Aktuganov G.E.1, Galimzyanova N.F.1, Teregulova G.A.1, Melentjev A.I.1
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隶属关系:
- Institute of Biology of Russian Academy of Sciences
- 期: 卷 52, 编号 5 (2016)
- 页面: 531-536
- 栏目: Article
- URL: https://journal-vniispk.ru/0003-6838/article/view/152019
- DOI: https://doi.org/10.1134/S0003683816050021
- ID: 152019
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A new strain Penicillium sp. IB-37-2, which actively hydrolyzes chitosan (SD ∼80–85%) but possesses low activity against colloidal chitin, was isolated. The fungus was observed to have a high level chitosanase biosynthesis (1.5–3.0 U/mL) during submerged cultivation at 28°C, with a pH of 3.5–7.0 and 220 rpm in nutrient media containing chitosan or chitin from shells of crabs. Purification of the chitosanase enzyme complex from Penicillium sp. IB-37-2 by ultrafiltration and hydrophobic chromatography, followed by denaturing electrophoresis, revealed two predominant proteins with molecular weights of 89 and 41 kDa. The purified enzyme complex demonstrated maximal activity (maximal rate of hydrolysis of dissolved chitosan) and stability at 50–55°C and a pH of 3.5–4.0. The enzyme preparation also hydrolyzed laminarin, β-(1,3)-(1,4)-glycan, and colloidal chitin. Exohydrolysis of chitosan by the preparation isolated from Penicillium sp. IB-37-2 resulted in the formation of single product, D-glucosamine.
作者简介
G. Aktuganov
Institute of Biology of Russian Academy of Sciences
编辑信件的主要联系方式.
Email: gleakt@anrb.ru
俄罗斯联邦, Ufa, Bashkortostan, 450054
N. Galimzyanova
Institute of Biology of Russian Academy of Sciences
Email: gleakt@anrb.ru
俄罗斯联邦, Ufa, Bashkortostan, 450054
G. Teregulova
Institute of Biology of Russian Academy of Sciences
Email: gleakt@anrb.ru
俄罗斯联邦, Ufa, Bashkortostan, 450054
A. Melentjev
Institute of Biology of Russian Academy of Sciences
Email: gleakt@anrb.ru
俄罗斯联邦, Ufa, Bashkortostan, 450054
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