PEGylated recombinant L-asparaginase from Erwinia carotovora: Production, properties, and potential applications
- Authors: Melik-Nubarov N.S.1, Grozdova I.D.1, Lomakina G.Y.1, Pokrovskaya M.V.2, Pokrovski V.S.2, Aleksandrova S.S.2, Abakumova O.Y.2, Podobed O.V.2, Grishin D.V.2, Sokolov N.N.2
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Affiliations:
- Department of Chemistry
- Orekhovich Scientific Research Institute of Biomedical Chemistry
- Issue: Vol 53, No 2 (2017)
- Pages: 165-172
- Section: Article
- URL: https://journal-vniispk.ru/0003-6838/article/view/152184
- DOI: https://doi.org/10.1134/S0003683817020119
- ID: 152184
Cite item
Abstract
N-hydroxysuccinimide ester of monomethoxy polyethylene glycol hemisuccinate was synthesized. It acylated amino groups in a molecule of recombinant L-asparaginase from Erwinia carotovora. A method of L-asparaginase modification by the obtained activated polyethylene glycol derivative was developed. The best results were produced by modification of the enzyme with a 25-fold excess of reagent relative to the enzyme tetramer. The modified L-asparaginase was isolated from the reaction mixture by gel filtration on Sepharose CL-6B. The purified bioconjugate did not contain PEG unbound to the protein, demonstrated high catalytic activity, and exhibited antiproliferative action on cell cultures.
About the authors
N. S. Melik-Nubarov
Department of Chemistry
Author for correspondence.
Email: melik.nubarov@genebee.msu.ru
Russian Federation, Moscow, 119991
I. D. Grozdova
Department of Chemistry
Email: melik.nubarov@genebee.msu.ru
Russian Federation, Moscow, 119991
G. Yu. Lomakina
Department of Chemistry
Email: melik.nubarov@genebee.msu.ru
Russian Federation, Moscow, 119991
M. V. Pokrovskaya
Orekhovich Scientific Research Institute of Biomedical Chemistry
Email: melik.nubarov@genebee.msu.ru
Russian Federation, Moscow, 119121
V. S. Pokrovski
Orekhovich Scientific Research Institute of Biomedical Chemistry
Email: melik.nubarov@genebee.msu.ru
Russian Federation, Moscow, 119121
S. S. Aleksandrova
Orekhovich Scientific Research Institute of Biomedical Chemistry
Email: melik.nubarov@genebee.msu.ru
Russian Federation, Moscow, 119121
O. Yu. Abakumova
Orekhovich Scientific Research Institute of Biomedical Chemistry
Email: melik.nubarov@genebee.msu.ru
Russian Federation, Moscow, 119121
O. V. Podobed
Orekhovich Scientific Research Institute of Biomedical Chemistry
Email: melik.nubarov@genebee.msu.ru
Russian Federation, Moscow, 119121
D. V. Grishin
Orekhovich Scientific Research Institute of Biomedical Chemistry
Email: melik.nubarov@genebee.msu.ru
Russian Federation, Moscow, 119121
N. N. Sokolov
Orekhovich Scientific Research Institute of Biomedical Chemistry
Email: melik.nubarov@genebee.msu.ru
Russian Federation, Moscow, 119121
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