PEGylated recombinant L-asparaginase from Erwinia carotovora: Production, properties, and potential applications


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Abstract

N-hydroxysuccinimide ester of monomethoxy polyethylene glycol hemisuccinate was synthesized. It acylated amino groups in a molecule of recombinant L-asparaginase from Erwinia carotovora. A method of L-asparaginase modification by the obtained activated polyethylene glycol derivative was developed. The best results were produced by modification of the enzyme with a 25-fold excess of reagent relative to the enzyme tetramer. The modified L-asparaginase was isolated from the reaction mixture by gel filtration on Sepharose CL-6B. The purified bioconjugate did not contain PEG unbound to the protein, demonstrated high catalytic activity, and exhibited antiproliferative action on cell cultures.

About the authors

N. S. Melik-Nubarov

Department of Chemistry

Author for correspondence.
Email: melik.nubarov@genebee.msu.ru
Russian Federation, Moscow, 119991

I. D. Grozdova

Department of Chemistry

Email: melik.nubarov@genebee.msu.ru
Russian Federation, Moscow, 119991

G. Yu. Lomakina

Department of Chemistry

Email: melik.nubarov@genebee.msu.ru
Russian Federation, Moscow, 119991

M. V. Pokrovskaya

Orekhovich Scientific Research Institute of Biomedical Chemistry

Email: melik.nubarov@genebee.msu.ru
Russian Federation, Moscow, 119121

V. S. Pokrovski

Orekhovich Scientific Research Institute of Biomedical Chemistry

Email: melik.nubarov@genebee.msu.ru
Russian Federation, Moscow, 119121

S. S. Aleksandrova

Orekhovich Scientific Research Institute of Biomedical Chemistry

Email: melik.nubarov@genebee.msu.ru
Russian Federation, Moscow, 119121

O. Yu. Abakumova

Orekhovich Scientific Research Institute of Biomedical Chemistry

Email: melik.nubarov@genebee.msu.ru
Russian Federation, Moscow, 119121

O. V. Podobed

Orekhovich Scientific Research Institute of Biomedical Chemistry

Email: melik.nubarov@genebee.msu.ru
Russian Federation, Moscow, 119121

D. V. Grishin

Orekhovich Scientific Research Institute of Biomedical Chemistry

Email: melik.nubarov@genebee.msu.ru
Russian Federation, Moscow, 119121

N. N. Sokolov

Orekhovich Scientific Research Institute of Biomedical Chemistry

Email: melik.nubarov@genebee.msu.ru
Russian Federation, Moscow, 119121

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