Analysis of interaction of bacterial cells and bacteriophages in conducting suspensions with an acoustic sensor


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Abstract

The possibility of analyzing bacterial cells infected by a specific bacteriophage, using Escherichia coli as an example, with an acoustic sensor directly in suspensions with different initial electrical conductivities was studied. The analysis was based on measurement of the time dependence of phase and the complete loss of output sensor signals of fixed frequency before and after biological interaction of microbial cells and bacteriophages. The aforementioned sensor makes it possible to detect bacterial cells and assess their viability in conducting suspensions. It was shown that the conductivity of the buffer solution should not exceed 10 μS/cm and the minimum detectable concentration of microbial cells was ~104 cells/mL.

About the authors

O. I. Guliy

Institute of Biochemistry and Physiology of Plants and Microorganisms; Vavilov Saratov State Agrarian University; Saratov Research Veterinary Institute

Author for correspondence.
Email: guliy_olga@mail.ru
Russian Federation, Saratov, 410049; Saratov, 410012; Saratov, 410028

B. D. Zaitsev

Kotel’nikov Institute of Radio Engineering and Electronics, Saratov Branch

Email: guliy_olga@mail.ru
Russian Federation, Saratov, 410019

I. A. Borodina

Kotel’nikov Institute of Radio Engineering and Electronics, Saratov Branch

Email: guliy_olga@mail.ru
Russian Federation, Saratov, 410019

A. M. Shikhabudinov

Kotel’nikov Institute of Radio Engineering and Electronics, Saratov Branch

Email: guliy_olga@mail.ru
Russian Federation, Saratov, 410019

A. A. Teplykh

Kotel’nikov Institute of Radio Engineering and Electronics, Saratov Branch

Email: guliy_olga@mail.ru
Russian Federation, Saratov, 410019

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