Vanillin Resistance Induced by BssS Overexpression in Escherichia coli


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Abstract

Overexpression of the BssS gene, a biofilm formation regulator, in planktonic Escherichia coli cells has been shown to confer the vanillin-resistant phenotype Vanr to the bacteria. The MG1655PL-tac-bssS strain started growing in liquid aerated LB medium with 2 g/L vanillin after a lag phase of 17 ± 2 h, whereas the original MG1655 strain did not grow under these conditions. The role of aldehyde reductase YqhD, a vanillin- degrading enzyme, in Vanr phenotype formation has been assessed. However, the Vanr trait in the MG1655PL-tac-bssS strain primarily depended on autoinducer-2 (AI-2), which formed in E. coli cells with an intact luxS gene. We supposed that BssS acts together with autoinducer-2 (which presumably accumulated during the prolonged lag phase) to induce vanillin resistance determined by changes in the expression of a range of genes.

About the authors

M. V. Ovsienko

Ajinomoto Genetika Research Institute (ZAO AGRI)

Author for correspondence.
Email: Maxim_Ovsienko@agri.ru
Russian Federation, Moscow, 117545

E. N. Fedorova

Ajinomoto Genetika Research Institute (ZAO AGRI)

Email: Maxim_Ovsienko@agri.ru
Russian Federation, Moscow, 117545

V. G. Doroshenko

Ajinomoto Genetika Research Institute (ZAO AGRI)

Email: Maxim_Ovsienko@agri.ru
Russian Federation, Moscow, 117545

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