Vanillin Resistance Induced by BssS Overexpression in Escherichia coli
- Authors: Ovsienko M.V.1, Fedorova E.N.1, Doroshenko V.G.1
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Affiliations:
- Ajinomoto Genetika Research Institute (ZAO AGRI)
- Issue: Vol 54, No 1 (2018)
- Pages: 21-25
- Section: Article
- URL: https://journal-vniispk.ru/0003-6838/article/view/152395
- DOI: https://doi.org/10.1134/S0003683818010088
- ID: 152395
Cite item
Abstract
Overexpression of the BssS gene, a biofilm formation regulator, in planktonic Escherichia coli cells has been shown to confer the vanillin-resistant phenotype Vanr to the bacteria. The MG1655PL-tac-bssS strain started growing in liquid aerated LB medium with 2 g/L vanillin after a lag phase of 17 ± 2 h, whereas the original MG1655 strain did not grow under these conditions. The role of aldehyde reductase YqhD, a vanillin- degrading enzyme, in Vanr phenotype formation has been assessed. However, the Vanr trait in the MG1655PL-tac-bssS strain primarily depended on autoinducer-2 (AI-2), which formed in E. coli cells with an intact luxS gene. We supposed that BssS acts together with autoinducer-2 (which presumably accumulated during the prolonged lag phase) to induce vanillin resistance determined by changes in the expression of a range of genes.
About the authors
M. V. Ovsienko
Ajinomoto Genetika Research Institute (ZAO AGRI)
Author for correspondence.
Email: Maxim_Ovsienko@agri.ru
Russian Federation, Moscow, 117545
E. N. Fedorova
Ajinomoto Genetika Research Institute (ZAO AGRI)
Email: Maxim_Ovsienko@agri.ru
Russian Federation, Moscow, 117545
V. G. Doroshenko
Ajinomoto Genetika Research Institute (ZAO AGRI)
Email: Maxim_Ovsienko@agri.ru
Russian Federation, Moscow, 117545
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